Gene chip detection method based on mixed probe

Abstract

The invention discloses a gene chip detection method based on mixed probes. The gene chip detection method comprises the following steps: controlling the effectiveness of a prepared gene chip by using two different fluorescent markers, mixing target probes into relatively short quality control probes marked by a first fluorescent group so as to prepare a sample application solution, performing sample application on the sample application solution on a substrate modified with arm molecules so as to form probe spots, hybridizing a sequence which is marked by a second fluorescent group and is to be detected with the target probes, and detecting fluorescent signals transmitted from the spots, wherein the first fluorescent group is used for marking and detecting whether the target probes are fixed on the chip substrate or not, and the second fluorescent group is used for marking and detecting the sequence information of the sequence to be detected. When two types of fluorescence are simultaneously used, whether the target probes are fixed on the substrate or not can be rapidly, easily and conveniently detected, so that the experiment result of false-negative caused by unfixed target probes is excluded.

Description

technical field [0001] The invention relates to a gene chip quality control method, in particular to a gene chip detection method based on mixed probes. Background technique [0002] As a biological high-tech developed rapidly in recent years, gene chip technology provides a bright prospect for how to study the functions of genes in the course of life. Gene chip technology refers to immobilizing a large number of probe molecules on a support and then hybridizing with labeled sample molecules, and obtaining the sequence information of the sample by detecting the hybridization signal intensity of each probe molecule. [0003] Compared with the traditional nucleic acid blot hybridization method, the gene chip immobilizes a large number of pre-designed probes on the surface of the support according to the characteristics and detection requirements of the target gene. One hybridization can detect and analyze the relevant information of multiple target genes in the sample. It...

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Problems solved by technology

However, none of the above methods can directly and effectively evaluate the quality of chip preparation.

[0007] These traditional gene chip quality control methods can reduce the possibility of false positive results in genetic testing and improve the accuracy of test results to a certa

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