Gene chip detection method based on temperature difference probes

A technology of gene chip and detection method, which is applied in the field of gene chip detection based on temperature difference probe, and achieves the effect of exquisite design and simple operation.

Inactive Publication Date: 2015-04-29
SOUTHEAST UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the deficiencies in the prior art, the present invention provides a detection method based on temperature difference probe gene chip, which can directly detect whether the probe is fixed on the substrate or not. On-chip, to eliminate the possibility of negative results due to lack of probes, improving the accuracy and reliability of gene chip detection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Gene chip detection method based on temperature difference probes
  • Gene chip detection method based on temperature difference probes
  • Gene chip detection method based on temperature difference probes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1: Transgenic soybean detection gene chip

[0062] 1. Sample preparation:

[0063] Preparation of Target Probe 5'-NH of Transgenic Soybean 2 - AAA AAA AAA AAA AAA CTG AAC GGG AAA CGA CAA TCT G-3', and a solution of the oligomer sequence 5'-Cy5-TTT TTT TTT TTT TTT-3' labeled with the first fluorophore Cy5, the The above two spotting solutions were thoroughly mixed to prepare a spotting solution.

[0064] 2. Gene chip preparation:

[0065] The above-mentioned mixed spotting solution is spotted on an aldehyde-based sheet, and the aldehyde-based sheet is prepared from a glass sheet treated with silane and glutaraldehyde to form a substrate with an aldehyde group modified at the end, and N probe spots are formed on the aldehyde-based sheet. N is a natural number, after fixing, at a low temperature of 25°C, the 5'-NH of the target probe in all the probe spots 2 -AAA AAA AAA AAA AAA CTG AAG GCG GGA AAC GAC AAT CTG-3' hybridizes with the Cy5-labeled oligomer sequenc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a gene chip detection method based on temperature difference probes. The gene chip detection method comprises the following steps: controlling the quality of a gene chip by using two different fluorescent markers; at relatively low temperature, firstly, hybridizing target probes with an oligomer sequence marked by a first fluorescent group marker, detecting the first fluorescent group, and judging whether the target probes are fixed on a substrate or not; at relatively high temperature, hybridizing the target probes with a DNA sequence which is marked by a second fluorescent group and is to be detected, detecting the second fluorescent group, and judging the detected DNA sequence information. When two types of fluorescence are simultaneously used, whether the target probes are fixed on the substrate or not can be rapidly, easily and conveniently detected, so that the experiment result of false-negative caused by unfixed target probes is avoided, and the accuracy and reliability of the gene detection result are improved.

Description

Technical field: [0001] The invention belongs to the field of gene chip quality control, in particular to a gene chip detection method based on temperature difference probes. Background technique: [0002] As a biological high-tech developed rapidly in recent years, gene chip technology provides a bright prospect for how to study the functions of genes in the course of life. Gene chip technology refers to immobilizing a large number of probe molecules on the support and hybridizing with labeled sample molecules, and then obtaining the number and sequence information of sample molecules by detecting the hybridization signal intensity of each probe molecule. [0003] Compared with the traditional nucleic acid blot hybridization method, the gene chip immobilizes a large number of pre-designed probes on the surface of the support according to the characteristics and detection requirements of the target gene. One hybridization can detect and analyze the relevant information of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6837C12Q2565/607C12Q2525/107C12Q2565/518C12Q2563/107
Inventor 刘全俊侯传荣于静静余筠如包镇汪荣亮谷德健
Owner SOUTHEAST UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap