Gene chip detection method based on temperature difference probes

Abstract

The invention discloses a gene chip detection method based on temperature difference probes. The gene chip detection method comprises the following steps: controlling the quality of a gene chip by using two different fluorescent markers; at relatively low temperature, firstly, hybridizing target probes with an oligomer sequence marked by a first fluorescent group marker, detecting the first fluorescent group, and judging whether the target probes are fixed on a substrate or not; at relatively high temperature, hybridizing the target probes with a DNA sequence which is marked by a second fluorescent group and is to be detected, detecting the second fluorescent group, and judging the detected DNA sequence information. When two types of fluorescence are simultaneously used, whether the target probes are fixed on the substrate or not can be rapidly, easily and conveniently detected, so that the experiment result of false-negative caused by unfixed target probes is avoided, and the accuracy and reliability of the gene detection result are improved.

Description

Technical field: [0001] The invention belongs to the field of gene chip quality control, in particular to a gene chip detection method based on temperature difference probes. Background technique: [0002] As a biological high-tech developed rapidly in recent years, gene chip technology provides a bright prospect for how to study the functions of genes in the course of life. Gene chip technology refers to immobilizing a large number of probe molecules on the support and hybridizing with labeled sample molecules, and then obtaining the number and sequence information of sample molecules by detecting the hybridization signal intensity of each probe molecule. [0003] Compared with the traditional nucleic acid blot hybridization method, the gene chip immobilizes a large number of pre-designed probes on the surface of the support according to the characteristics and detection requirements of the target gene. One hybridization can detect and analyze the relevant information of ...

AI Technical Summary

Problems solved by technology

[0008] Aiming at the deficiencies in the prior art, the present invention provides a detection method based on temperature difference probe gene chip, which can directly detect whether the probe is fixed on the substrate or not. On-chip, to eliminate the possibility of negative results due to lack of probes, improving the accuracy and reliability of gene chip detection

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