Novel method for preparing transparent skeleton specimen and storing resin

A technology for making transparent bones and specimens, applied in the field of biology, can solve the problems of inconvenient transportation, specimen fading, moldy, etc., and achieve the effects of easy display and preservation, high transparency, and short production cycle

Inactive Publication Date: 2015-05-06
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, at present, the production cycle of transparent bone specimens is long, and because there is only one storage method of liquid glycerin, the reasons for the preservation of this method will cause the specimens to fade, become moldy, reduce the transparency, and the transportation is inconvenient, and the solution needs to be replaced regularly. These follow-up problems are greatly Inhibit the development of transparent bone specimens in biology teaching, museum collections, and popular science activities

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0019] (1) Pretreatment: collect fresh animal carcasses, that is, those dead within one day, remove the epidermis, and open a small opening from the upper end of the cloaca, remove all internal organs, remove eyeballs for large mammals, and remove gills and scales for fish.

[0020] (2) Dehydration: fix the specimen, immerse the collected specimen in 95% ethanol and dehydrate for 1-5 days. The number of dehydration days depends on the size of the animal. If it is fish, it will take 1-2 days; for large mammals, it will take 3-5 days Days, depending on the epidermis pigment faded.

[0021] (3) Bleaching: remove the melanin therein, and bleach for 1-2 hours with 3% hydrogen peroxide by mass.

[0022] (4) Transparency: Soak in 3% potassium hydroxide for 2-5 days, depending on its size. If it is a larger animal, you can inject 5% potassium hydroxide solution into the thicker part of the muscle to speed up the transparency of the specimen. speed.

[0023] (5) Dyeing: The mass rati...

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Abstract

The invention discloses a novel method for preparing a transparent skeleton specimen and storing resin. The method comprises the following steps: pretreatment, dehydration, bleaching, hyalinizing, dyeing, destaining, hyalinizing again, glycerin removing, drying, sealing for storage and the like. According to the method, the problems that the specimen fades and goes moldy caused by the storage method at present, the transparency is reduced, the specimen is inconvenient to transport, and a solution needs to be exchanged at regular intervals are solved. According to the method, kinds of problems in existing transparent skeleton specimen preparation and storage are overcome; the method has the advantage of short preparation cycle; and the prepared specimen has the advantages of being easy to show and store, and high in transparency, does not go bad or go moldy, and can be stored for a long period of time.

Description

technical field [0001] The invention belongs to the technical field of biology and relates to a technology for making a novel resin transparent bone specimen. Background technique [0002] The study of vertebrate skeleton is not only an important content of animal sports physiology, but also an important basis for animal classification. Transparent bone specimens can show structures that are difficult to see clearly with general anatomical methods. It can display the skeletal part of the body while keeping the shape of the specimen intact, showing the skeletal development of the organism. The production principle is to apply chemicals and physical methods to the body tissue, so that the refractive index of the tissue is similar to that of the transparent agent, so as to achieve the purpose of transparency. The prepared specimens are vivid, easy to observe the bones in situ, have a relatively strong three-dimensional sense, and can show the natural distribution and position...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/00
Inventor 蒋韦斌赵惠东唐仕华俞伟东
Owner SHANGHAI NORMAL UNIVERSITY
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