Redback christmashush extract, and preparation method and application thereof in preparation of drugs for treating hepatitis B

A technology of red-backed leaf root and extract, which is applied in the direction of drug combination, pharmaceutical formula, plant raw materials, etc., can solve the problem of long maintenance time, and achieve the effect of simple and reasonable extraction process

Active Publication Date: 2015-05-06
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented method allows for easy access into an organism's roots by taking them out from their skin without causing harm or discomfort during testing procedures.

Problems solved by technology

This patents describes various methods for studying how antibodies against viruses cause damage to cells during their use on humans. These techniques include immune response testing, cell culture experiments, histological examinations, gene analysis, Western blotting technology, molecular pharmacokines, chemical reactions, biochemistry, etc., which aim to identify specific parts responsible for causing diseases such as acute inflammation syndrome, liver failure, bone fractures, cholestatis fevers, lung scirms, pulmonary abscesses, steatosis niche syndromes, heart attacks, gastrointestinal troubles, kidney stone formation, cardiovascular system issues like arterial thromboembolia, renal impairment, lipid accumulation, and secondary organ injury. However, these technical problem addressed in this patented method involves identifying potential targets involved in the action mechanism of substances called ribancycles associated with hepatocelebrushimannuclidium complex(RBL)/nucleotide DNA binding site (NBTB)-related genome sequences, specifically Laminobacterium spore-forming activa borne negative nitrosourethanoindones (DNRIA)) and other nucleic acid analogs derived therefrom.

Method used

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  • Redback christmashush extract, and preparation method and application thereof in preparation of drugs for treating hepatitis B

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preparation example Construction

[0022] The present invention is a kind of preparation method of red back leaf root extract, and it comprises the following steps:

[0023] (1) The red-backed leaf root medicinal material is extracted with 6-12 times the amount of ethanol for 1-2 hours, extracted 1-3 times, combined with the ethanol extract, filtered and concentrated under reduced pressure. Preferably, the ethanol concentration in step (1) is 60%-90%, more preferably, the ethanol concentration is 70%-80%. The root of the red-backed leaf is the root of the dicotyledonous plant Euphorbiaceae plant Alchornea trewioides (Benth.) Muell.–Arg.

[0024] (2) Centrifuge the concentrate obtained in step (1), and concentrate the supernatant under reduced pressure for subsequent use.

[0025] (3) Put the concentrate obtained in step (2) on a medium polar or nonpolar macroporous adsorption resin column at a flow rate of 1.3-1.7 column volume / hour.

[0026] (4) After removing impurities by eluting with deionized water, elut...

Embodiment 1

[0031] The red-backed leaf root medicinal material was reflux extracted twice with ethanol, the ethanol concentration was 75%, the amount of alcohol added was 10 times the weight of the medicinal material, and the extraction time was 90 minutes. The extracts were combined, filtered and concentrated under reduced pressure, the concentrate was centrifuged, and the supernatant was concentrated under reduced pressure for later use.

[0032] Take a medium-polarity macroporous adsorption resin, pack it into a column at a ratio of 1:2 to the crude drug by weight, and then pass the above-mentioned standby concentrated solution through the macroporous resin column at a flow rate of 1.5 column volume / hour. After passing through completely, use Deionized water was used for elution, and then 30-50% ethanol was used for elution, and the elution flow rate was 2.5 column volumes / hour. The ethanol eluate was collected, dried under reduced pressure, and pulverized to obtain the root extract of...

Embodiment 2

[0034] The red-backed leaf root medicinal material was reflux extracted with ethanol for 3 times, the ethanol concentration was 90%, the amount of alcohol added was 12 times of the weight of the medicinal material, and the extraction time was 120 min. The extracts were combined, filtered and concentrated under reduced pressure, the concentrate was centrifuged, and the supernatant was concentrated under reduced pressure for later use.

[0035] Take a medium-polarity macroporous adsorption resin, pack it into a column at a ratio of 1:2 to the crude drug by weight, and then pass the above-mentioned standby concentrated solution through the macroporous resin column at a flow rate of 1.5 column volume / hour. After passing through completely, use Deionized water was used for elution, and then 30-60% ethanol was used for elution, and the elution flow rate was 2.5 column volumes / hour. The ethanol eluate was collected, dried under reduced pressure, and pulverized to obtain the root extr...

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Abstract

The invention discloses a redback christmashush extract, and a preparation method and application thereof in preparation of drugs for treating hepatitis B. The preparation method comprises the steps of carrying out alcohol extraction, centrifugal concentration, macroporous adsorption resin column treatment, ethanol elution and reduced pressure drying, so as to obtain an effective part extract of redback christmashush. The preparation method has the beneficial effects that an extraction process is simple, convenient and reasonable; by applying the obtained effective part extract of redback christmashush in an anti-duck hepatitis B virus experiment, serum DHBV DNA can be remarkably inhibited on the seventh day and the fourteenth day (T7 and T14) by virtue of three doses of effective part extracts of redback christmashush (P is less than 0.01), and DHBV DNA rises again on fifth day (P5) after the drug delivery is stopped and is still remarkably lower than that before the drug delivery, so that the redback christmashush extract has a relatively ideal anti-hepatitis B virus effect, is a candidate drug with great promise and can be applied to the preparation of anti-hepatitis B drugs.

Description

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Claims

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Application Information

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Owner SOUTHERN MEDICAL UNIVERSITY
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