Method for constructingmouse animal model with qi-deficiency typegastrointestinal dysfunction

A technology for gastrointestinal dysfunction and animal model, applied in the directions of pharmaceutical formulations, plant raw materials, preparations for in vivo experiments, etc., can solve problems such as difficulty in clarifying the pathogenesis, and achieve easy promotion, high stability, and good repeatability. Effect

Active Publication Date: 2015-05-20
浙江中医药大学附属第一医院
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Through long-term clinical observation, it is found that many critically ill patients have severe gastrointestinal dysfunction without obvious trauma, infection, bleeding and other stress conditions, often manifested as abdominal distension, diarrhea and severe malnutrition. Conforms to the clinical manifestations of gastrointestinal dysfunction but is difficult to be classified into the above three types of gastrointestinal dysfunction, and it is difficult for Western medicine to clarify its pathogenesis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for constructingmouse animal model with qi-deficiency typegastrointestinal dysfunction
  • Method for constructingmouse animal model with qi-deficiency typegastrointestinal dysfunction
  • Method for constructingmouse animal model with qi-deficiency typegastrointestinal dysfunction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: the preparation of medicine

[0017] (1) Sijunzi Decoction: Codonopsis 9g, Atractylodes macrocephala 9g, Poria cocos 9g, roasted licorice 6g

[0018] Each crude drug was soaked in water for 30 minutes, and boiled by the Chinese Pharmacy of Zhejiang Provincial Hospital of Traditional Chinese Medicine to contain 500 mg of crude drug per mL. According to the body surface coefficient conversion method, the mouse dose equivalent to the human clinical dose is 42.9mg / 10g·d (the calculation formula is: mouse dosage g / kg·d=human daily dose*0.0026 / 0.02).

[0019] (2) Glauber's salt supersaturated aqueous solution: Glauber's salt (sodium sulfate decahydrate Na 2 SO 4 10H 2 O) Pass through a 20-mesh sieve after crushing, gradually add to room temperature (about 20°C) distilled water, stir with a magnetic stirrer until Glauber's salt crystals are precipitated, and then stir for 15 minutes, the precipitated crystals are no longer dissolved, and the solution at this t...

Embodiment 2

[0020] Embodiment 2: the construction and verification of animal model

[0021] 1. Grouping of animals

[0022] There were 120 healthy adult male ICR mice, weighing 18-22g, randomly selected 24 as the control group, 24 as the modeling placebo group, and 72 as the experimental group according to the digital random table. The mice in the experimental group were randomly divided into model group, self-healing group and Sijunzi decoction group, 24 in each group.

[0023] 2. Group administration method

[0024] The control group was fed with normal diet for 10 days, and the modeling placebo group was given normal diet with normal saline for 10 days by intragastric administration at the same time. The mice in the experimental group were fed a normal diet with a dose of 0.3ml / 10g of Glauber's salt supersaturated aqueous solution orally for 10 days. After 10 days, some mice in the control group, the modeling placebo group, and the model group were killed immediately (observation of...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for constructing a mouse animal model with a qi-deficiency typegastrointestinal dysfunction. The method comprises the following steps: lavaging a mouse with a supersaturatedmirabilitewater solution for 10-15 days, then lavaging with a sijunzi decoction for 7-10 days, and successfully constructing the mouse animal model with the qi-deficiency typegastrointestinal dysfunction when the mouse has the symptoms in (1), (4) and (6) or more as follows: (1) the weight is reduced, and the fur of the mouse becomes grey, is free of color and luster; (2) the food-intake is reduced; (3) excrement becomes thin; (4) intestinal mucosal injury score becomes poor; (5) the volume of the stomach is increased; and (6) the gastrointestinal carbon powder propulsion index declines. The invention provides the mouse animal model with the qi-deficiency typegastrointestinal dysfunction, which is good in stability, easy to popularize, good in repeatability, economic and practical; and an experimentfoundation is provided for clinical earlier research on treatment of the gastrointestinal dysfunction with traditional Chinese medicines.

Description

(1) Technical field [0001] The present invention relates to the establishment of an animal model of gastrointestinal dysfunction due to qi deficiency. By feeding ICR mice with a supersaturated solution of mirabilite, the mice can produce abdominal distension, diarrhea, weight loss and other clinical manifestations of qi deficiency in traditional Chinese medicine, as well as gastrointestinal motility decline, The clinical manifestations and pathological changes of Western medicine gastrointestinal dysfunction such as gastrointestinal mucosal injury provide a stable and reliable animal model for the development of new drugs for the treatment of gastrointestinal dysfunction. (2) Background technology [0002] Gastrointestinal dysfunction refers to the severe stress response of the gastrointestinal tract when the body is severely damaged by trauma, infection, and blood loss. The intestinal barrier function is destroyed, and the bacteria and bacterial toxins in the intestine are ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/484A61K49/00
Inventor 黄立权王灵聪江荣林雷澍智屹惠吴艳春朱美飞
Owner 浙江中医药大学附属第一医院
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap