Method for constructingmouse animal model with qi-deficiency typegastrointestinal dysfunction

Abstract

The invention discloses a method for constructing a mouse animal model with a qi-deficiency typegastrointestinal dysfunction. The method comprises the following steps: lavaging a mouse with a supersaturatedmirabilitewater solution for 10-15 days, then lavaging with a sijunzi decoction for 7-10 days, and successfully constructing the mouse animal model with the qi-deficiency typegastrointestinal dysfunction when the mouse has the symptoms in (1), (4) and (6) or more as follows: (1) the weight is reduced, and the fur of the mouse becomes grey, is free of color and luster; (2) the food-intake is reduced; (3) excrement becomes thin; (4) intestinal mucosal injury score becomes poor; (5) the volume of the stomach is increased; and (6) the gastrointestinal carbon powder propulsion index declines. The invention provides the mouse animal model with the qi-deficiency typegastrointestinal dysfunction, which is good in stability, easy to popularize, good in repeatability, economic and practical; and an experimentfoundation is provided for clinical earlier research on treatment of the gastrointestinal dysfunction with traditional Chinese medicines.

Description

(1) Technical field [0001] The present invention relates to the establishment of an animal model of gastrointestinal dysfunction due to qi deficiency. By feeding ICR mice with a supersaturated solution of mirabilite, the mice can produce abdominal distension, diarrhea, weight loss and other clinical manifestations of qi deficiency in traditional Chinese medicine, as well as gastrointestinal motility decline, The clinical manifestations and pathological changes of Western medicine gastrointestinal dysfunction such as gastrointestinal mucosal injury provide a stable and reliable animal model for the development of new drugs for the treatment of gastrointestinal dysfunction. (2) Background technology [0002] Gastrointestinal dysfunction refers to the severe stress response of the gastrointestinal tract when the body is severely damaged by trauma, infection, and blood loss. The intestinal barrier function is destroyed, and the bacteria and bacterial toxins in the intestine are ...

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Problems solved by technology

[0004] Through long-term clinical observation, it is found that many critically ill patients have severe gastrointestinal dysfunction without obvious trauma, infection, bleeding and other stress conditions, often manifested as abdominal distension, diarrhea and severe malnutrition. Conforms to the clinical manifestations of gastrointestinal dysfunction but is difficult to be classified into the above three types of gastrointestinal dysfunction, and it is difficult for Western medicine to clarify its pathogenesis

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