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Detection method and kit for serum thiol total oxidation capacity

A detection method and technology of oxidative capacity, which are applied in the field of serum biological indicators to achieve the effects of good serum volume dependence and good reaction time dependence

Active Publication Date: 2016-08-17
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

At present, there are more than 5,000 literatures on serum total antioxidant capacity, among which 2,267 are related to diseases (sourced from CNKI), which mainly evaluate the influence of lifestyle, environment and disease on the level of total antioxidant capacity, which is useless. Total antioxidant capacity as a basis for diagnosing diseases or evaluating health status

Method used

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  • Detection method and kit for serum thiol total oxidation capacity
  • Detection method and kit for serum thiol total oxidation capacity
  • Detection method and kit for serum thiol total oxidation capacity

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Embodiment Construction

[0037] Now, the present invention will be described in detail in conjunction with the accompanying drawings and embodiments.

[0038] The embodiment of the present invention includes using the kit to study the time effect and dose effect of serum thiol oxidation, detecting the serum thiol total oxidation capacity of 40 healthy human samples and detecting the serum thiol total oxidation capacity of 32 healthy mouse samples.

[0039] The kit reagents of the present invention include: a) reaction buffer solution; b) reaction matrix solution; c) reaction termination solution; d) color development solution. Wherein the reaction buffer is selected as the hydroxyethylpiperazine ethylsulfuric acid buffer containing ethylenediaminetetraacetic acid; the reaction matrix liquid is dithiothreitol solution; the reaction termination solution is guanidine hydrochloride solution; Substituted dinitrobenzoic acid buffer.

[0040] Embodiment According to the kit operation method of the present i...

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Abstract

The invention relates to the field of biological indexes of serum, and more particularly relates to a detection method and kit of serum thiol total oxidation capacity. According to the detection method and kit, a serum thiol total oxidation capacity concept is put forwarded and a measurement method is established. The measurement method comprises the following step: by taking dithiothreitol, polypeptide containing thiol or protein as reaction matrix liquid, and taking dithiobisnitro benzoic acid as an indicator of residual thiol, measuring the total oxidation capacity of thiol in serum. Comprehensive indexes of such a novel development can be helpful for understanding the thiol total oxidation capacity of the body. The detection method disclosed by the invention discovers that the total oxidation capacities of different species of serum thiol have large differences, and the same species of the serum also have different total oxidation capacities on different individuals.

Description

technical field [0001] The invention relates to the field of serum biological indicators, which is used for evaluating the comprehensive index of serum thiol total oxidation ability, and specifically relates to a detection method and a kit for serum thiol total oxidation ability. technical background [0002] Serum contains a variety of antioxidant enzymes, such as catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase, glutathione transferase, thioredoxin and sulfur redoxin reductase. There are also many small antioxidant molecules in serum, such as glutathione, vitamin C and vitamin E. On the other hand, serum contains various oxidases, such as NADPH oxidase and myeloperoxidase, etc. There are also many small molecules with oxidative capacity in serum, such as lipid peroxide and hydrogen peroxide. Faced with the complex background of the coexistence of oxidation and reduction, people put forward the comprehensive index of serum total antioxidant c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/52
CPCG01N21/78
Inventor 张劲松孙康
Owner ANHUI AGRICULTURAL UNIVERSITY
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