New Application of Type I Collagen α1 Chain
A technology of gene and lung adenocarcinoma, applied in the field of application in the preparation of drugs for the treatment of non-small cell lung cancer
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Embodiment 1
[0023] Example 1 Meta-analysis of high-throughput transcriptome data in lung adenocarcinoma
[0024] We normalized the results of 15 sets of lung adenocarcinoma gene chips that have been reported, and then performed t-test to screen differentially expressed genes. A total of 1063 differentially expressed genes were screened out, of which 464 genes were up-regulated in expression level. 599 down-regulated genes. In order to better study the functions of differentially expressed genes, we performed GO functional enrichment and KEGG pathway enrichment on differentially expressed genes by DAVID. The results of KEGG pathway enrichment showed that 37 genes could be screened out in the KEGG library (see Table 1), focusing on the focal adhesion signaling pathway, which may be related to the metastasis of lung adenocarcinoma. Based on our research direction, COL1A1 was selected as a candidate gene for classical molecular biology experiment verification.
[0025] Table 1 Signaling pat...
Embodiment 2
[0028] Example 2 Lung adenocarcinoma tissue and normal tissue COL1A1 gene expression
[0029] 1. Materials and methods
[0030] 1. Materials
[0031]Lung adenocarcinoma tissues and normal tissues were obtained from hospitalized cases from 2005 to 2010, and 5 cases were obtained respectively.
[0032] 2. Method
[0033] 2.1 Extraction of total RNA from lung adenocarcinoma tissue and normal tissue
[0034] The RNA of the tissue was extracted according to the instructions of the Trizol kit, the integrity of the RNA was proved by gel electrophoresis, and the concentration and purity of the RNA was determined with a nucleic acid protein analyzer. Total RNA extraction kit was used to extract. The main operation steps are as follows:
[0035] (1) Lyse the tissue cells with cell lysis solution BL, centrifuge to get the upper layer cells, add 1 ml of Trizol to the upper layer cells, and pump the lysate 10 times with a disposable syringe with a needle;
[0036] (2) After standing ...
Embodiment 3
[0080] Example 3 Expression of COL1A1 gene in non-small cell lung cancer tissues and paracancerous tissues
[0081] 1. Materials and methods
[0082] 1. Materials
[0083] Non-small cell lung cancer was collected from hospitalized cases from 2005 to 2010. 134 lung adenocarcinoma tissues and adjacent tissues were collected and numbered separately.
[0084] 2. Method
[0085] Refer to the experimental method in Example 2.
[0086] two results
[0087]Among the 134 samples of non-small cell lung cancer, the expression level of COL1A1 in cancer tissue was higher than that in adjacent tissue in 133 samples, and the expression level of COL1A1 in cancer tissue was not clearly different from that in adjacent tissue in only one sample. The accuracy rate is 99%, indicating that the COL1A1 gene has a good correlation with lung adenocarcinoma, and can be used to prepare auxiliary diagnosis or prognosis preparations for lung adenocarcinoma.
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