Counting method and counting system for milk body cells based on mobile equipment

A milk somatic cell and mobile device technology, which is applied in the field of on-site fluorescent milk somatic cell counting, can solve the problems of high cost, inconvenient on-site detection, and large detection system volume, and achieve low cost, convenient on-site detection, and elimination of complexity. The effect of the process

Active Publication Date: 2015-05-27
CHONGQING UNIV
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Problems solved by technology

[0006] Aiming at the problems of large volume, high cost and inconvenient on-site detection in the existing detection technology, the purpose of the present invention is to provide a milk somatic cell counting method based on mobile equipment

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  • Counting method and counting system for milk body cells based on mobile equipment
  • Counting method and counting system for milk body cells based on mobile equipment
  • Counting method and counting system for milk body cells based on mobile equipment

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Embodiment Construction

[0030] In order to better understand the present invention, the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0031] One, a method for counting milk somatic cells based on a mobile device, comprising the steps of:

[0032] 1) Sampling treatment, labeling treatment of milk somatic cells on the milk sample to be tested;

[0033] 2) Induce fluorescence amplification treatment to enhance the fluorescence of labeled milk somatic cells;

[0034] 3) Use mobile devices to obtain cell images and perform counting algorithm processing to identify and count milk somatic cells, and display, transmit or print them;

[0035] Wherein, the mobile device is an intelligent mobile device such as a mobile phone or a tablet computer, and the pictures are obtained by taking pictures and the milk somatic cell counting processing of the pictures is performed. Convert binary image, count and display counting results by 8-ne...

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Abstract

The invention provides a counting method and a counting system for milk body cells based on mobile equipment. The method comprises the following steps: sample processing: marking the milk body cells for a to-be-tested milk sample; induced fluorescent amplification treatment: carrying out fluorescence enhancement on the marked milk body cells; and acquiring cell images by using mobile equipment and processing by virtue of a counting algorithm to identify the milk body cells and carry out statistics on quantity, and displaying, transmitting or printing, wherein the mobile equipment acquires images by virtue of photographing and counts the milk body cells of the images. The processing method sequentially comprises the following steps: carrying out RGB to gray processing on the images; converting the grayscale image to a binary image; carrying out breadth first search and counting; and displaying a counting result. The method and the system provided by the invention have the characteristics of small size, portability, high analysis efficiency and good precision, can be used for field detection of a milk plant and the like.

Description

technical field [0001] The invention belongs to fluorescent milk somatic cell detection technology, in particular to a mobile device-based method and system for on-site fluorescent milk somatic cell counting. Background technique [0002] Milk somatic cell count (scc) refers to the total number of somatic cells per milliliter of milk. Cow mastitis is the main reason for the increase of somatic cell count in milk. Studies have shown that there is a close correlation between mastitis in dairy cows and somatic cell count in milk. When mastitis occurs in dairy cows, the somatic cell count generally exceeds 500,000 / mL, and the milk production drops to about one-tenth of the original. This is one of the most serious diseases that cause dairy companies to lose money. At present, milk somatic cell count has become an international indicator for diagnosing milk udder inflammation. my country stipulates that the number of somatic cells in fresh milk per milliliter is less than or e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14G01N1/14
Inventor 郭永彩刘晓竹刘茂莲
Owner CHONGQING UNIV
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