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Radix radix c21 steroid extract and its preparation method and use

A technology of extract and radish, applied in the field of medicine, can solve problems such as no product, no research report on multidrug resistance reversal agent of radix C21 steroid tumor cells, etc.

Inactive Publication Date: 2017-11-21
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported in the literature that C21 steroid compounds have been isolated and identified from Radix Radix, and the C21 steroid compounds isolated and identified from Radix Radix have so far included three kinds of deacyl radixenin derivatives and glycosides, and nobilein aglycone derivatives. 20 kinds of glucosides, 5 kinds of ramamone aglycon derivatives and glycosides, 1 kind of sarcobalonein, 1 kind of desarcoclidinin (Warashina T, et al . Phytochemistry , 1998, 49: 2103-2108.; Warashina T, et al . Chems Pharm Bull , 1998, 46: 1752-1757.), but there is no product with high content of Radix C21 steroid extract; there is no research report on the preparation of tumor cell multidrug resistance reversal agent by Radix Radix C21 steroid

Method used

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  • Radix radix c21 steroid extract and its preparation method and use
  • Radix radix c21 steroid extract and its preparation method and use
  • Radix radix c21 steroid extract and its preparation method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Method for Determination of C21 Steroid Weight Percentage in Radix Radix C21 Steroid Extract by Colorimetry

[0058] Solution preparation

[0059] Preparation of reference solution

[0060] Accurately weigh an appropriate amount of nodolimonin reference substance, add methanol to make each 1 ml containing 198.72 mu g solution, as the reference solution.

[0061] Preparation of the test solution

[0062] Take 0.1 g of radish C21 steroid extract, weigh it accurately, add methanol to dissolve, transfer to a 50 ml measuring bottle, add methanol to the mark, shake well, accurately draw 10 ml, put it in a 100 ml measuring bottle, add methanol to scale, shake well, filter, and take the continued filtrate as the test solution.

[0063] Assay method

[0064] Precisely draw the test solution 100 mu l, put it in a stoppered test tube, evaporate the solvent, add 5 ml of freshly prepared concentrated sulfuric acid-methanol (6:1) precisely, shake well, put it in a 60°C water ...

Embodiment 2

[0066] Take 100 Kg of dried cane powder of radish, add 8 times the amount of water to reflux and extract 3 times, each time for 2 hours, filter, combine the extracts, recover ethanol under reduced pressure to obtain the extract. Take the extract and dissolve it in water, add it to the HPD100 macroporous adsorption resin column, elute with water until the eluent is nearly colorless, discard the eluent, and elute with 10% ethanol until the eluent is nearly colorless, discard the eluent Remove the liquid, elute with 90% ethanol until the eluate is nearly colorless, collect the eluate, add the 90% ethanol eluate to the D941 decolorization resin column, elute with 6 times the column volume of 90% ethanol, collect the eluate liquid, concentrated under reduced pressure, and dried to obtain 1.47 Kg of Radix Radix C21 steroid extract, wherein the weight percentage of C21 steroid is 52%.

Embodiment 3

[0068] Take 100 Kg of dried cane powder of radish, add 8 times the amount of 30% ethanol to reflux and extract 3 times, each time for 2 hours, filter, combine the extracts, and recover the ethanol under reduced pressure to obtain the extract. Take the extract and dissolve it in water, add it to HPD300 macroporous adsorption resin column, elute with water until the eluent is nearly colorless, discard the eluent, and elute with 50% ethanol until the eluent is nearly colorless, discard the eluent Remove the liquid, elute with 60% ethanol until the eluate is almost colorless, collect the eluate, add the 60% ethanol eluate to the D941 decolorization resin column, elute with 6 times the column volume of 90% ethanol, collect the eluate liquid, concentrated under reduced pressure, and dried to obtain 0.46 Kg of Radix Radix C21 steroid extract, wherein the weight percentage of C21 steroid is 91%.

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Abstract

The invention provides a Radix Radix C21 steroid extract, a preparation method and application thereof. The dried cane of dill radix is ​​extracted with 0-100% ethanol solution, and the C21 steroid of dill radix is ​​separated by the method of macroporous adsorption resin and decolorization resin, wherein the weight percentage of C21 steroid is 50-95%. The obtained radish C21 steroid extract has been proved by pharmacological activity studies to have the effect of reversing tumor multidrug resistance, and can be used to prepare tumor cell multidrug resistance reversing agent.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to a Radix Radix C21 steroid extract and a preparation method thereof, and also relates to the use of the Radix Radix Radix C21 steroid extract in preparing a tumor cell multidrug resistance reversal agent. Background technique [0002] Malignant tumor is one of the diseases that seriously endanger human health. It is characterized by abnormal cell proliferation and metastasis. Statistics from the World Health Organization show that in 2000, there were about 10 million new cases of malignant tumors in the world, 6.2 million deaths, and 22 million cases of illness; it is estimated that by 2020, there will be 15 million new cases of malignant tumors, 10 million deaths, and 30 million illnesses. Thousands of cases. Chemotherapy is one of the methods of comprehensive therapy for malignant tumors. It is widely used before, during and after surgery. However, the phenomenon of multidrug resistance o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/27A61P35/00
Inventor 王威刘小红韩立春颜世达姚慧丽
Owner QINGDAO UNIV
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