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Construction method and detection method of fingerprint of Dongfeng orange medicinal material
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A fingerprint and construction method technology, applied in the field of medicinal material detection, can solve the problems of insufficient intuitive and comprehensive results and large errors, and achieve the effects of stable results, easy operation and high precision
Active Publication Date: 2016-09-07
GUANGZHOU XINGQUN PHARMA
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[0003] Dongfengju is the root of the tree. At present, there are many confusing products in Chinese medicinal materials that are similar in appearance to it. However, the existing methods for detecting whether it is Dongfengju are relatively complicated. And other studies to identify, the results are not intuitive and comprehensive enough, the error is large
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Embodiment 1
[0042] This example is a method for constructing the fingerprint of Dongfengju medicinal material.
[0047] Glacial acetic acid (chromatographically pure, Tianjin Institute of Chemical Reagents); methanol, acetonitrile (chromatographically pure, Honeywell Company); water was ultrapure water.
[0048] 3. Materials
[0049] Dongfeng orange medicinal material (provided by Guangzhou Baiyunshan Xingqun (Pharmaceutical) Co., Ltd., a total of 12 batches, the batch numbers or origins are 20110501, 20111201, XG130701, XG111102, XG110501, XG140401, XG111002, 20110401, XG110401, 20140501, Guangdong, Guangxi ).
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Embodiment 2
[0144] The detection method of a kind of Dongfeng orange medicinal material fingerprint of present embodiment comprises the following steps:
[0145] (1) Preparation of the test solution:
[0146] Take about 0.5g of the medicinal material powder to be tested (batch number: 20140306, provided by Guangzhou Baiyunshan Xingqun (Pharmaceutical) Co., Ltd., passed through a 60-mesh sieve), accurately weigh it, put it in a 100ml conical flask with a stopper, and add methanol precisely 10ml, plugged tightly, weighed, refluxed at 70°C for 45 minutes, allowed to cool, weighed again, made up for the lost weight with methanol, shaken well, filtered through a 0.22μm microporous membrane, and the filtrate was obtained.
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Abstract
The invention relates to a construction method and a detection method for an atalantia buxjfolia medicinal material finger-print. The construction method comprises the following steps: precisely weighing atalantia buxjfolia medicinal material powder, precisely adding methanol, carrying out reflux extraction or ultrasonic extraction for 45-60 minutes, and filtering to obtain a test solution; and precisely sucking the test solution, injecting the test solution into a high performance liquid chromatograph and testing the test solution to obtain the atalantia buxjfolia medicinal material finger-print consisting of 18 characteristic peaks. The atalantia buxjfolia medicinal material finger-print has 18 characteristic peaks capable of relatively comprehensively representing the physical and chemical characteristics of the atalantia buxjfolia medicinal material, so that the atalantia buxjfolia medicinal material can be accurately and intuitively identified, and moreover, the method has the advantages of being simple to operate, stable in result, high in precision, good in reproducibility and the like.
Description
technical field [0001] The invention relates to the field of medicinal material detection, in particular to a construction method and a detection method of a fingerprint medicinal map of dongfengju. Background technique [0002] Dongfengju is the root of Rutaceae Bougainvillea. It is fragrant, slightly pungent and bitter in taste, and warm in nature. It has the effects of expelling wind and cold, promoting qi and relieving pain, and is used to treat bronchitis, wind-cold cough, cold and fever, rheumatoid arthritis, chronic gastritis, gastric ulcer, swelling and pain from falls, etc. [0003] Dongfengju is the root of the tree. At present, there are many confusing products in Chinese medicinal materials that are similar in appearance to it. However, the existing methods for detecting whether it is Dongfengju are relatively complicated. For example, through the traits and microscopic observation of the roots and stems of Dongfengju And other studies to identify, the results a...
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