Bacillus megaterium strain and application thereof

A technology of Bacillus megaterium and strains, applied in Bacillus megaterium strains and its application fields, can solve the problems of increased production cost and long production cycle

Inactive Publication Date: 2015-07-22
INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Not to mention the large loss of enzymes in this process, the use of this production procedu

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  • Bacillus megaterium strain and application thereof
  • Bacillus megaterium strain and application thereof
  • Bacillus megaterium strain and application thereof

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[0022] A Bacillus megaterium strain, the strain is Bacillus megaterium strain BM5 (Bacillus megaterium), which was deposited in the General Microbiology Center of the China Microbial Species Collection Management Committee on February 28, 2014, and the deposit number is CCMCC NO.8866.

[0023] The Bacillus megaterium strain BM5 can be used in the treatment of tofu wastewater, and it also produces endoglucanase and xylanase composite enzyme preparations. The specific method is as follows: collect tofu wastewater discarded in the production of tofu, and adjust the pH of the tofu wastewater To 7.0, place in a fermenter for autoclave sterilization at 121°C for 25 minutes, and reduce the Bacillus megaterium strain BM5 to 10 6 The inoculation amount of cfu / mL was inoculated in the fermentor, aerated, and cultured at 33°C for 3 days. The tofu wastewater was fermented into a complex enzyme solution containing endoglucanase and xylanase.

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Abstract

The invention discloses a Bacillus megaterium strain and application thereof. The strain is Bacillus megaterium strain BM5, is preserved in the Chinese Culture Collection Committee General Microbiology Center on February 28, 2014, and has a preservation number of CCMCC NO.8866; the application of the Bacillus megaterium strain comprises the following steps: collecting tofu wastewater, adjusting pH to 7.0, placing the tofu wastewater in a fermentation tank to perform autoclaved sterilization for 25 min at 121 DEG C, inoculating 106 cfu/mL of Bacillus megaterium strain BM5 in the fermentation tank to perform aeration, culturing the strain for 3 days at 33 DEG C to obtain a composite enzyme solution through fermentation. According to the invention, the Bacillus megaterium strain can ferment and grow by using the tofu wastewater as the unique nutrient, and can produce the enzyme solutions of endoglucanase and xylanase, so as to provide a new fermentation bacteria source for the production of endoglucanase and xylanase preparations.

Description

Technical field [0001] The invention relates to a Bacillus megaterium strain and its application. Background technique [0002] In order to make full use of resources and save costs, substances with high cellulose and hemicellulose content, such as rice bran, wheat flour, cottonseed meal, straw meal, and pasture, are often used as raw materials to replace high-priced corn, soybeans, and wheat. However, a high proportion of crude fiber diets not only cannot be directly absorbed and utilized by livestock and poultry, but also easily cause various problems such as diarrhea and slow growth of livestock and poultry. In order to improve feed quality, feed mills and farms have fully realized the importance of adding enzymes to feed, especially non-digestible enzymes such as cellulase and hemicellulase that cannot be synthesized by animals themselves. [0003] The main components of plant cell walls are composed of cellulose, hemicellulose and lignin. Cellulose and hemicellulose are cross...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/42A23K1/165C12R1/11
Inventor 林斌官雪芳徐庆贤钱蕾
Owner INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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