Promoter and recombinant expression vector as well as application thereof and method for expressing heterologous proteins

A technology for expressing vectors and foreign proteins, applied in the biological field, can solve the problem of low transcriptional activity and achieve the effect of increasing expression

Active Publication Date: 2015-07-22
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the existing defects of lower transcriptional activity of promoters

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Promoter and recombinant expression vector as well as application thereof and method for expressing heterologous proteins
  • Promoter and recombinant expression vector as well as application thereof and method for expressing heterologous proteins
  • Promoter and recombinant expression vector as well as application thereof and method for expressing heterologous proteins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] This example is used to illustrate the method for obtaining the promoter of the present invention.

[0032] The DNA was prepared by using Tiangen bacterial genome extraction reagent, operating according to its instructions, and using Bacillus subtilis WB600 strain. Using the primers shown in Table 1, the prepared DNA was amplified, and the amplified products were DNA sequenced. The results showed that the amplified products were of different lengths with restriction endonuclease sites and protective groups. The promoter shown in SEQ IE NO.1-5.

[0033] Table 1

[0034]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a promoter. The length of the promoter is 150-1000bp, wherein the promoter comprises a nucleic acid fragment expressed as SEQ ID NO.1; the promoter is capable of starting the transcription in bacillus subtilis. The invention also provides a recombinant expression vector; a reading frame is allowed to be inserted into the recombinant expression vector and is used for expressing protein in bacteria; a promoter of the recombinant expression vector comprises the promoter. The invention further provides a method for expressing heterologous proteins. The method comprises a step of inserting the reading frame for encoding the heterologous proteins into the recombinant expression vector to prepare recombinant expression plasmids. The invention also provides application of the promoter to preparation of the recombinant expression vector and/or expressing the proteins in the plasmids. By virtue of the technical scheme, the expression quantity of the heterologous proteins can be obviously increased without induction.

Description

Technical field [0001] The present invention relates to the field of biotechnology, in particular to a promoter, a recombinant expression vector, a method for expressing foreign proteins and the use of the promoter. Background technique [0002] A promoter is a DNA sequence located in the upstream region of the 5'end of a structural gene, which can activate RNA polymerase to accurately bind to the template DNA and have the specificity of transcription initiation. Because the specific transcription of a gene depends on whether the enzyme and the promoter can effectively form a binary complex, how the RNA polymerase effectively finds the promoter and combines with it is the first problem to be solved in the transcription initiation process. Experiments have shown that for many promoters, the rate at which RNA polymerase binds to it is at least 100 times higher than random collisions in Brownian motion. [0003] The transcriptional activity of different promoters is quite different. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/113C12N15/63C12N15/75C12R1/125
Inventor 田健伍宁丰余小霞刘晓青初晓宇
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap