Application of dienestrol antibody in simultaneous detection of various strol
A technology of diethylstilbestrol and diethylstilbestrol, which is applied in the directions of measuring devices, biological tests, material inspection products, etc., can solve the problems of cumbersome process operation, unsuitable for rapid primary screening, inability to realize on-site detection, etc., and achieves low detection limit , the effect of a wide linear range
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Embodiment 1
[0016] The detection of embodiment 1 to diethylstilbestrol
[0017] The immunoelectrochemical sensor modified by nano-gold / diethylstilbestrol / graphene / chitosan composite was immersed in a total volume of 50 μL of phosphate containing different concentrations of free diethylstilbestrol and diethylstilbestrol antibody with a titer concentration of 1:6400. Buffer solution, incubated at 37°C for 30 minutes, rinsed with phosphate buffer solution, and then placed in 2mmol / L K 3 [Fe(CN) 6 ] solution for differential pulse voltammetry (DPV) scanning.
[0018] The DPV peak current of the modified electrode after being incubated in the incubation solution with a diethylstilbestrol concentration of 0 is defined as I 0 , the DPV peak current of the modified electrode after being incubated in the incubation solution containing different concentrations of diethylstilbestrol is defined as I X , calculate ΔI=I X -I 0 , the ΔI-C working curve can be obtained by plotting ΔI against the die...
Embodiment 2
[0019] Embodiment 2 to the detection of diethylstilbestrol
[0020] The immunoelectrochemical sensor modified by nano-gold / stilbestrol / graphene / chitosan composite was immersed in a total volume of 50 μL containing different concentrations of free stilbestrol, and a titer concentration of 1:6400 In the phosphate buffer solution of diethylstilbestrol antibody, incubate at 37°C for 40 minutes, rinse with phosphate buffer solution, and incubate in 2mmol / L K 3 [Fe(CN) 6 ] solution for differential pulse voltammetry (DPV) scanning.
[0021] The DPV peak current of the modified electrode after being incubated in the incubation solution with a diethylstilbestrol concentration of 0 is defined as I 0 , the DPV peak current of the modified electrode after being incubated in the incubation solution containing different concentrations of diethylstilbestrol is defined as I X , calculate ΔI=I X -I 0 , the ΔI-C working curve can be obtained by plotting ΔI against the concentration of die...
Embodiment 3
[0022] Determination of standard-added hexestrol in the pork sample of embodiment 3
[0023] 1) Pork sample processing: Weigh 1±0.0050g pork into a 10mL sample tube, add diethylstilbestrol standard solution, and 3mL acetonitrile-acetone extraction solution (V:V=4:1), the mixture is ultrasonically oscillated for 30 minutes, Centrifuge at 2000r / m for 10 minutes, transfer the supernatant to a nitrogen blowpipe, repeat the extraction of the residue once with 3 mL of the same extraction solution, and combine the supernatants into a nitrogen blowpipe. The extract was concentrated and evaporated at 50°C under nitrogen blowing, and the concentrate was dissolved in 1 mL of phosphate buffer solution with a pH of 7.4 for electrochemical analysis.
[0024] 2) Determination of spiked hexestrol in pork samples: Take equal amounts of different pork extract samples, mix with hexestrol antibody solution and phosphate buffer solution to make an incubation solution, so that the total volume is 50 ...
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