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Application of dienestrol antibody in simultaneous detection of various strol

A technology of diethylstilbestrol and diethylstilbestrol, which is applied in the directions of measuring devices, biological tests, material inspection products, etc., can solve the problems of cumbersome process operation, unsuitable for rapid primary screening, inability to realize on-site detection, etc., and achieves low detection limit , the effect of a wide linear range

Inactive Publication Date: 2015-08-12
SHANGHAI OCEAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these analytical methods require large-scale analytical instruments, the process operation is cumbersome, and on-site detection cannot be realized. It is necessary to study and establish more efficient, fast and sensitive rapid detection methods
Immunoassay is one of the most effective and rapid detection methods, but at present, most of the immunoassays for estrol are specific analysis and detection, that is, each detection method can only detect a specific compound, which is not suitable for rapid primary screening. Therefore, simultaneous detection of multiple The multi-residue immunoassay method for estrol has become a key technology that needs to be developed urgently, among which antibodies that can simultaneously recognize multiple estrols are the key link

Method used

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  • Application of dienestrol antibody in simultaneous detection of various strol
  • Application of dienestrol antibody in simultaneous detection of various strol
  • Application of dienestrol antibody in simultaneous detection of various strol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The detection of embodiment 1 to diethylstilbestrol

[0017] The immunoelectrochemical sensor modified by nano-gold / diethylstilbestrol / graphene / chitosan composite was immersed in a total volume of 50 μL of phosphate containing different concentrations of free diethylstilbestrol and diethylstilbestrol antibody with a titer concentration of 1:6400. Buffer solution, incubated at 37°C for 30 minutes, rinsed with phosphate buffer solution, and then placed in 2mmol / L K 3 [Fe(CN) 6 ] solution for differential pulse voltammetry (DPV) scanning.

[0018] The DPV peak current of the modified electrode after being incubated in the incubation solution with a diethylstilbestrol concentration of 0 is defined as I 0 , the DPV peak current of the modified electrode after being incubated in the incubation solution containing different concentrations of diethylstilbestrol is defined as I X , calculate ΔI=I X -I 0 , the ΔI-C working curve can be obtained by plotting ΔI against the die...

Embodiment 2

[0019] Embodiment 2 to the detection of diethylstilbestrol

[0020] The immunoelectrochemical sensor modified by nano-gold / stilbestrol / graphene / chitosan composite was immersed in a total volume of 50 μL containing different concentrations of free stilbestrol, and a titer concentration of 1:6400 In the phosphate buffer solution of diethylstilbestrol antibody, incubate at 37°C for 40 minutes, rinse with phosphate buffer solution, and incubate in 2mmol / L K 3 [Fe(CN) 6 ] solution for differential pulse voltammetry (DPV) scanning.

[0021] The DPV peak current of the modified electrode after being incubated in the incubation solution with a diethylstilbestrol concentration of 0 is defined as I 0 , the DPV peak current of the modified electrode after being incubated in the incubation solution containing different concentrations of diethylstilbestrol is defined as I X , calculate ΔI=I X -I 0 , the ΔI-C working curve can be obtained by plotting ΔI against the concentration of die...

Embodiment 3

[0022] Determination of standard-added hexestrol in the pork sample of embodiment 3

[0023] 1) Pork sample processing: Weigh 1±0.0050g pork into a 10mL sample tube, add diethylstilbestrol standard solution, and 3mL acetonitrile-acetone extraction solution (V:V=4:1), the mixture is ultrasonically oscillated for 30 minutes, Centrifuge at 2000r / m for 10 minutes, transfer the supernatant to a nitrogen blowpipe, repeat the extraction of the residue once with 3 mL of the same extraction solution, and combine the supernatants into a nitrogen blowpipe. The extract was concentrated and evaporated at 50°C under nitrogen blowing, and the concentrate was dissolved in 1 mL of phosphate buffer solution with a pH of 7.4 for electrochemical analysis.

[0024] 2) Determination of spiked hexestrol in pork samples: Take equal amounts of different pork extract samples, mix with hexestrol antibody solution and phosphate buffer solution to make an incubation solution, so that the total volume is 50 ...

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Abstract

The invention discloses an application of a dienestrol antibody in an immune electrochemical detection method of simultaneous detection of three female hormone analogous compounds, namely, diethylstilbestrol, dienestrol and hexestrol. The detection method includes following steps: (1) preparing an electrochemical sensor deposited by nano gold and modified by a graphene / strol / chitosan composition; (2) with K3[Fe(CN)6] as a probe, performing the simultaneous detection of the three strol on the basis of a cross immunoreaction of the dienestrol antibody to the three strol. In the method, a detection limit is 0.2 ng / mL and a linearity range is 1-4000 ng / mL of the diethylstilbestrol; the detection limit is 0.05 ng / mL and the linearity range is 10-4000 ng / mL of the dienestrol; and the detection limit is 0.5 ng / mL and the linearity range is 100-4000 ng / mL of the hexestrol.

Description

technical field [0001] The invention belongs to the technical field of food safety detection and analytical chemistry, and relates to a diethylstilbestrol antibody capable of simultaneously recognizing three similar compounds of female hormones, i.e., diethylstilbestrol, diethylstilbestrol and diethylstilbestrol. The application in detection is specifically an immunoelectrochemical detection method based on antibody cross-reaction, and the application in the simultaneous detection of the above three estrogen-like compounds contained in food. Background technique [0002] Diethylstilbestrol (DES), diethylstilbestrol (DE) and hexestrol (HEX) (see structural formula figure 1 ) belong to 1,2-stilbene drugs, which are synthetic estrogens. Stilbene hormones can promote animal growth, increase feed conversion rate, and reduce fat synthesis. In the 1960s, they were officially approved as growth-promoting agents by various countries for animal husbandry production. However, the abu...

Claims

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Application Information

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IPC IPC(8): G01N33/74G01N27/327
CPCG01N33/74G01N27/3278
Inventor 张洁王传现赵波王锡昌邵科峰任硕刘夏李晓虹吴珺张红琳
Owner SHANGHAI OCEAN UNIV
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