An electrochemical immunoassay method for detecting estrol
An immunodetection method and electrochemical technology, applied in the field of electrochemical immunodetection based on antibody cross-reaction, can solve the problems of cumbersome process operation, inability to realize on-site detection, etc., and achieve the effect of low detection limit
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Embodiment 1
[0026] Embodiment 1 The detection of diethylstilbestrol by electrochemical immunoassay method
[0027] Preparation of electrochemical immunosensor:
[0028] A glassy carbon electrode with a diameter of 3 mm was sequentially filled with Al with a diameter of 0.3 μm and 0.05 μm 2 o 3 Grinding with polishing powder, followed by ultrasonic cleaning with absolute ethanol-distilled water and distilled water for 5 minutes, and then rinsed with distilled water. First deposit nano-gold on the cleaned electrode by electrochemical deposition, then drop-coat 4 μL of graphene-chitosan dispersion on the electrode, and then drop-coat 2 μL of 0.2 mg / mL diethylstilbestrol solution on the electrode surface, 40 Dry at ℃. The modified electrode was soaked in 5% BSA solution and incubated in a 37 °C oven for 30 min to block the remaining active sites.
[0029] The electrochemical immunosensor modified by nano-gold / diethylstilbestrol / graphene / chitosan composite was immersed in a total volume of...
Embodiment 2
[0031] Embodiment 2 The detection of hexestrol by electrochemical immunoassay method
[0032] Preparation of electrochemical immunosensor:
[0033] A glassy carbon electrode with a diameter of 3 mm was sequentially filled with Al with a diameter of 0.3 μm and 0.05 μm 2 o 3 Grinding with polishing powder, followed by ultrasonic cleaning with absolute ethanol-distilled water and distilled water for 5 minutes, and then rinsed with distilled water. First deposit nano-gold on the cleaned electrode by electrochemical deposition, then drop-coat 4 μL of graphene-chitosan dispersion on the electrode, and then drop-coat 2 μL of 0.2 mg / mL hexestrol solution on the electrode Surface, dry at 40°C. The modified electrode was soaked in 5% BSA solution and incubated in a 37 °C oven for 30 min to block the remaining active sites.
[0034] The electrochemical immunosensor modified by nano-gold / stilbestrol / graphene / chitosan composite was immersed in a total volume of 50 μL containing antibodie...
Embodiment 3
[0036] Example 3 Electrochemical immunoassay method for the detection of bisphenol A
[0037] Preparation of electrochemical immunosensor:
[0038] A glassy carbon electrode with a diameter of 3 mm was sequentially filled with Al with a diameter of 0.3 μm and 0.05 μm 2 o 3 Grinding with polishing powder, followed by ultrasonic cleaning with absolute ethanol-distilled water and distilled water for 5 minutes, and then rinsed with distilled water. First, deposit nano-gold on the cleaned electrode by electrochemical deposition, then drop-coat 4 μL of graphene-chitosan dispersion on the electrode, and then drop-coat 2 μL of 0.2 mg / mL bisphenol A solution on the surface of the electrode , dried at 40°C. The modified electrode was soaked in 5% BSA solution and incubated in a 37 °C oven for 30 min to block the remaining active sites.
[0039] The electrochemical immunosensor modified by nano-gold / bisphenol A / graphene / chitosan composite was immersed in a total volume of 50 μL conta...
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