A method for simultaneously cultivating japonica rice and indica rice by using wild rice

Abstract

The invention discloses a method for cultivating Oryza sativa L.ssp.indica and Oryza sativaL.ssp.japonica simultaneously by using Oryza rufipogon Griff.. The method comprises the steps of parent hybridization, embryo rescue, backcross, self-cross and identification of Oryza sativa L.ssp.indica and Oryza sativaL.ssp.japonica, wherein the parent hybridization is that Yuanjiang Oryza rufipogon Griff. is used as a male parent, Oryza sativa L.ssp.indica is used as the female parent, and a pollen mixture obtained by mixing anthers of dead Oryza sativa L.ssp.indica with pollens of Oryza rufipogon Griff. according to a mass ratio of 1: 1 is used for pollination; an embryo culture medium comprises modified MS, 8 g / L of agar powder and 10 g / L of sucrose and has the pH of 5.8-6.0. According to the invention, the method greatly improves the success rate of hybridization, rescues the immature embryos obtained through hybridization, and improves the success rate of distant hybridization of cultivated rice and Oryza rufipogon Griff. from 0-3% to 60%-70%; through the synergy of all steps, Oryza sativa L.ssp.indica and Oryza sativaL.ssp.japonica are obtained simultaneously; the method is simple and practicable; a new method and technical support are provided for the cultivation of endemic new strains of Oryza sativa L.ssp.indica and Oryza sativaL.ssp.japonica.

Description

technical field [0001] The invention relates to a method for breeding japonica rice and indica rice, in particular to a method for simultaneously cultivating japonica rice and indica rice by using wild rice as a parent, and belongs to the technical field of crop breeding. Background technique [0002] Rice is the staple food crop of more than half of the world's population. The cultivated species are the Asian cultivated rice (Oryza sativa L.) which is widely cultivated in the world and the African cultivated rice (Oryza glaberrima steud.) which is limited to West Africa. In my country, the cultivated rice species are Rice cultivated in Asia is divided into two subspecies: indica rice (Oryza sativa L. ssp. indica) and japonica rice (Oryza sativa L. ssp. japonica). my country is a country that pays equal attention to japonica and indica in rice cultivation. As early as two thousand years ago, the existence of two subspecies of japonica and indica was recognized, and the layout...

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