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A chemically modified electrode for detecting cephalexin and an electrochemical determination method for cephalexin

A technology of chemical modification and determination method, applied in the field of electrochemical detection, can solve problems such as health hazards, and achieve the effects of low cost, high sensitivity and good selectivity

Active Publication Date: 2017-08-04
ZHONGKAI UNIV OF AGRI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If people eat this animal-derived food containing antibiotics for a long time, it will bring great harm to human health.

Method used

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  • A chemically modified electrode for detecting cephalexin and an electrochemical determination method for cephalexin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Preparation of a chemically modified electrode for detecting cephalexin

[0027] (1) Preparation of colloidal gold solution

[0028] Weigh a certain amount of chitosan and dissolve it in 1.0% acetic acid solution to make 30mL of 2mg / mL solution, add 15mL of 10mmol / L chloroauric acid solution under electromagnetic stirring, stir for 30min, then drop by drop under stirring Add 6mL of a newly prepared 0.1mol / L sodium borohydride aqueous solution and continue stirring until the solution turns into a transparent wine red (about 1.5h);

[0029] (2) Preparation of chemically modified electrodes

[0030] After the gold electrode is polished with polishing powders of different particle sizes, it is rinsed with water, and then put in 1:1 nitric acid solution, absolute ethanol and water in sequence, cleaned with an ultrasonic cleaner, and dried naturally. Drop 3μl of colloidal gold solution on the surface of the treated gold electrode. After drying at room temperature, immerse ...

Embodiment 2

[0031] Example 2 Preparation of chemically modified electrode for detecting cephalexin

[0032] (1) Preparation of colloidal gold solution

[0033] Weigh a certain amount of chitosan and dissolve it in 2.0% acetic acid solution to make 40mL of 3mg / mL solution, add 10mL of 20mmol / L chloroauric acid solution under electromagnetic stirring, stir for 40min, then drop by drop under stirring Add 5 mL of a newly prepared 0.2mol / L sodium borohydride aqueous solution and continue to stir until the solution turns into a transparent wine red (about 1.5h);

[0034] (2) Preparation of chemically modified electrodes

[0035] After the gold electrode is polished with polishing powders of different particle sizes, it is rinsed with water, and then placed in 1:1 nitric acid solution, absolute ethanol and water in sequence, cleaned with an ultrasonic cleaner, and dried naturally. Drop 3μl of colloidal gold solution on the surface of the treated gold electrode. After drying at room temperature, immerse...

Embodiment 3

[0036] Example 3 Preparation of a chemically modified electrode for detecting cephalexin

[0037] (1) Preparation of colloidal gold solution

[0038] Weigh a certain amount of chitosan and dissolve it in 0.05% acetic acid solution to make 20mL of 1mg / mL solution, add 20mL of 5mmol / L chloroauric acid solution under electromagnetic stirring, stir for 40min, then drop by drop under stirring Add 10mL of a newly prepared 0.05mol / L sodium borohydride aqueous solution and continue to stir until the solution turns into a transparent wine red (about 1.5h);

[0039] (2) Preparation of chemically modified electrodes

[0040] After the gold electrode is polished with polishing powders of different particle sizes, it is rinsed with water, and then placed in 1:1 nitric acid solution, absolute ethanol and water in sequence, cleaned with an ultrasonic cleaner, and dried naturally. Drop 3μl of colloidal gold solution on the surface of the treated gold electrode. After drying at room temperature, imme...

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Abstract

The invention relates to the technical field of electrochemical detection, and specifically discloses a chemically modified electrode for detecting cephalexin and an electrochemical determination method for cephalexin. The chemically modified electrode is prepared by a method comprising the following steps: drop a colloidal gold solution on the surface of the cleaned gold electrode, dry it and immerse it in a hydrochloric acid solution containing cysteine, and automatically Assemble, then take it out and rinse with water. The electrochemical determination method comprises the steps of: adding a copper sulfate solution to the sample solution to be tested for hydrolysis to obtain a hydrolyzate, using the above-mentioned chemically modified electrode as a working electrode, and measuring the remaining concentration in the hydrolyzate by square wave voltammetry The concentration of copper ions, thereby indirectly measuring the content of cephalexin in the sample. Using the chemically modified electrode prepared by the invention to measure cephalexin has the advantages of high sensitivity, quickness, convenience, low cost and the like.

Description

Technical field [0001] The invention relates to the technical field of electrochemical detection, in particular to a chemically modified electrode for detecting cephalexin and an electrochemical determination method of cephalexin. Background technique [0002] Cephalexin is a broad-spectrum antibiotic synthesized in the 1960s. It belongs to the first generation of cephalosporins. It improves the penetration of the cell wall of Gram-negative bacteria and reduces the affinity of binding proteins. The antibacterial spectrum of cephalexin is the same as that of broad-spectrum penicillin, and it has strong antibacterial activity against Staphylococcus, Streptococcus pneumoniae, Salmonella and Neisseria that produce or do not produce penicillinase. It is mainly used for infections of gram-positive and negative bacteria, and has a good bactericidal effect on penicillin-resistant fungi. [0003] Cephalosporins have been widely used in medicine and veterinary clinics because of their stron...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30G01N27/26
Inventor 赵晓娟黄桂颖白卫东陈海光刘功良李艺勤
Owner ZHONGKAI UNIV OF AGRI & ENG
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