Preparation method of swallow nest extractive, and obtained swallow nest extractive and application thereof
A technology of extract and bird's nest, which is applied in application, food preparation, skin care preparations, etc., can solve the problems of low product yield and complicated process, and achieve the effects of saving costs, extending the validity period, and improving stability
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[0035] The invention discloses a preparation method of a bird's nest extract, the prepared bird's nest extract and its application. Those skilled in the art can learn from the content of this article and appropriately improve the process parameters to realize it. In particular, it should be pointed out that all similar replacements and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The method and application of the present invention have been described through preferred embodiments, and the relevant personnel can obviously make changes or appropriate changes and combinations to the method and application described herein without departing from the content, spirit and scope of the present invention to realize and Apply the technology of the present invention.
[0036] The preparation method of the bird's nest extract provided by the present invention, the obtained bird's nest extract and the raw materi...
Embodiment 1
[0038] The preparation of embodiment 1 bird's nest extract
[0039] (1) Buy 20g of bird's nest from the market;
[0040] (2) Soak white swallow or yellow swallow in water (25°C, in g / L, the mass volume ratio of bird’s nest to water is 1:450) for about 3 hours, and soak blood swallow in warm water for about 9 hours;
[0041] (3) filter the bird's nest soaked in water with a 70-mesh sieve to obtain the bird's nest filter residue;
[0042] (4) Collect the bird's nest filter residue, repeatedly freeze and thaw 3 times in a refrigerator at 10°C and -80°C, and break the bird's nest filter residue;
[0043] (5) wash with water twice, each time 2min, filter respectively, collect the filtrate after washing;
[0044] (6) filter residue is collected simultaneously, with 0.4% neutral protease solution (neutral protease enzyme activity is: 300,000 U / g; the preparation of neutral protease solution: the neutral protease powder of 0.4mg is dissolved in the PBS of 100ml In the solution, fil...
Embodiment 2
[0049] The preparation of embodiment 2 bird's nest extract
[0050](1) Buy 20g of bird's nest from the market;
[0051] (2) Soak white swallow or yellow swallow in water (40°C, in g / L, the mass volume ratio of bird’s nest to water is 1:350) for about 8 hours, and soak blood swallow in warm water for about 18 hours;
[0052] (3) filter the bird's nest soaked in water with a 70-mesh sieve to obtain the bird's nest filter residue;
[0053] (4) Collect the bird's nest filter residue, repeatedly freeze and thaw 5 times in the refrigerator at 10°C to 35°C and -80°C, and break the bird's nest filter residue;
[0054] (5) Wash twice with water, each time for 5 minutes, filter respectively, and collect the filtrate after washing;
[0055] (6) filter residue is collected simultaneously, and with 0.2% neutral protease solution (neutral protease enzyme activity is: 300,000 U / g; the preparation of neutral protease solution: the neutral protease powder of 0.2mg is dissolved in the PBS of ...
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