Chitosanase production strain and application thereof

A technology of chitosan solution and chitosan oligosaccharide, which is applied in the field of microorganisms, can solve the problems of chitosan application limitations, etc., and achieve the effect of little impact on product quality, simple post-treatment, and less enzyme amount

Active Publication Date: 2015-09-16
迪嘉药业集团股份有限公司
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Problems solved by technology

[0002] Chitosan is the only natural alkaline polysaccharide found so far, but due to its large molecular weight and compact crystal struc

Method used

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  • Chitosanase production strain and application thereof
  • Chitosanase production strain and application thereof
  • Chitosanase production strain and application thereof

Examples

Experimental program
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Effect test

Embodiment

[0047] ① Bacillus 10066 Breeding of strains

[0048] The soil samples collected from the sea near Rongcheng were made into a 10% sample solution with sterile water. After standing still, the supernatant was taken, diluted in gradient, and spread on a plate medium with chitosan as the only carbon source. After culturing at 37°C for 2 days, 24 colonies with transparent circles were screened out.

[0049] After screening, 24 strains were obtained, and then re-screened in liquid medium, and cultured in a shaker at 37°C at 180r / min for 24h. The formula of the culture medium is: chitosan 0.3%, yeast extract powder 0.1%, peptone 1%, sodium chloride 0.3%.

[0050]The enzyme activity was determined by the 3,5-dinitrosalicylic acid method (DNS), and three strains with high enzyme activity were selected for stable passage for three generations, and the growth curve, enzyme production rate and fermentation broth degradation of the three strains were measured at the same time The produc...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to a microbial strain for producing chitosanase and a method for preparing chitosan oligosaccharide by using the strain. The chitosanase production strain is the Bacillus sp., which is deposited with China General Microbiological Culture Collection Center (CGMCC) on November 26, 2014 and is assigned the accession number of CGMCC No.10066. The use of the chitosanase production strain shortens the time of fermentation and enzyme production and improves the quality and production efficiency of the chitosan oligosaccharide.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, in particular to a microbial strain producing chitosanase and a method for preparing chitosan oligosaccharides using the chitosanase. Background technique [0002] Chitosan is the only natural alkaline polysaccharide found so far, but due to its large molecular weight and compact crystal structure, it is insoluble in common solvents and can only be dissolved in some acidic media, which makes chitosan application is severely limited. Studies have shown that the molecular weight has a great influence on the properties of chitosan, and the properties of chitosan with different molecular weights are very different, and sometimes even show diametrically opposite characteristics. Oligochitosan is a low-molecular-weight product of chitosan degradation, with a degree of polymerization between 2 and 10, and has high solubility and physiological activity. According to reports, chitosan oligosaccha...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/42C12P19/14C12R1/07
CPCC12N9/2402C12P19/14C12N1/205C12R2001/07
Inventor 李广生马芳芳黄丽君张明涛曲欣欣
Owner 迪嘉药业集团股份有限公司
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