The invention discloses a heat-clearing herbal fruit and vegetable juice enzyme, prepared from the fruits, 10-15 parts of lychee, 8-11 parts of pear, 7-9 parts of apple, 5-8 parts of banana, 10-14 parts of mango, 12-15 parts of orange, 13-15 parts of kumquat, 11-13 parts of pomelo, 15-20 parts of 'red globe' grape, 9-12 parts of starfruit, 14-16 parts of cherry, 10-12 parts of kiwifruit, 7-9 parts of watermelon, 6-9 parts of Hami melon, 13-17 parts of longan, 9-11 parts of muskmelon, 8-10 parts of pineapple, 14-20 parts of apricot, 12-15 parts of guava, and 15-17 parts of blueberry; and cereals and vegetables, 5-7 parts of sorghum, 3-5 parts of rice, 2-4 parts of bitter buckwheat, 4-6 parts of coix seed, 3-6 parts of pumpkin, 2-5 parts of mung bean, 4-7 parts of pea, 3-7 parts of cowpea, 2-6 parts of lentil, and 1-4 parts of black bean. By mixture fermentation of fruits, cereals, vegetables and Chinese herbs, the heat-clearing herbal fruit and vegetable juice enzyme can provide the functions of adjusting immune response, fighting cancer, lowering blood pressure, regulating intestinal tracts, eliminating constipation, resisting gout, relieving diabetics, lowering weight, fighting allergy and the like, and as well as the effects in Chinese medicine, such as clearing heat, disinhibiting dampness for relieving jaundice, and clearing heat and toxicity.