Aerobic and bacterial vaginosis combined determination kit

A technology for bacterial and vaginosis, applied in the field of joint determination kits for aerobic bacteria and bacterial vaginosis, which can solve the problems of complex methods, time-consuming, inconvenient use, etc.

Inactive Publication Date: 2015-09-30
浙江亚培生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commonly used method for detecting aerobic bacteria in vaginal secretions is the microbial culture method, which is to inoculate the vaginal secretions on

Method used

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Embodiment 1

[0029] A combined assay kit for aerobic bacteria / bacterial vaginosis, characterized in that it includes a chromogenic agent and a reaction device, and the reaction device includes a hydrogen peroxide reaction pad, a sialidase reaction pad, and a leukocyte esterase reaction pad , β-glucuronidase reaction pad and coagulase reaction pad.

[0030] The hydrogen peroxide reaction pad is coated with 4-aminoantipyridine, phosphate, 3,5 dichlorodihydroxybenzenesulfonic acid and peroxidase.

[0031] The sialidase reaction pad is coated with cresol ester, acetate and glacial acetic acid.

[0032] The leukocyte esterase reaction pad is coated with 5 bromo-4 chloro-3 indole acetate and phosphate.

[0033] The β-glucuronidase reaction pad is coated with 5 bromo-4 chloro-3 indolyl sodium β-D-glucuronate, phosphate and calcium chloride.

[0034] The coagulase reaction pad is coated with glycyl-arginyl 4-methyl-β-naphthylamine hydrochloride and tris-hydroxymethylaminomethane (tris-hcl).

[...

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PUM

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Abstract

The invention discloses an aerobic and bacterial vaginosis combined determination kit and a preparation method thereof. The kit comprises a color-developing agent and a reaction device, wherein the reaction device comprises a hydrogen oxide reaction pad, a neuraminidase reaction pad, a leucocyte esterase reaction pad, a beta-glucuronidase reaction pad and a coagulase reaction pad. Sample liquid is sucked by using straw, one drop of the sample liquid is dropwise added on each of the hydrogen oxide reaction pad, the neuraminidase reaction pad, the leucocyte esterase reaction pad, the beta-glucuronidase reaction pad and the coagulase reaction pad of the reaction device, the volume of each drop of the sample liquid is 30 mu l, after the reaction device is put into a water bath at a temperature of 36-38 DEG C or a dry bath at a temperature of 48 DEG C to carry out color developing for 15 minutes, one drop of color-developing liquid A is added on the neuraminidase reaction pad, one drop of color-developing liquid B is added on the coagulase reaction pad, and 30 seconds later, a result is shown. Thus, an effect of simultaneously detecting hydrogen oxide, neuraminidase, leucocyte esterase, beta-glucuronidase and coagulase to diagnose aerobic and bacterial vaginosis can be achieved.

Description

technical field [0001] The invention relates to a kit, in particular to a kit for combined determination of aerobic bacteria and bacterial vaginosis and a preparation and use method. Background technique [0002] Aerobic vaginitis is a newly discovered vaginitis in recent years, accounting for about 20% of vaginal infections. The main pathogenic bacteria are Group B Streptococcus, Enterococcus faecalis, Staphylococcus aureus and Escherichia coli. At present, the commonly used method for detecting aerobic bacteria in vaginal secretions is the microbial culture method, which is to inoculate the vaginal secretions on a blood plate, separate and identify them after cultivation. This method is complicated, time-consuming, and inconvenient for clinical routine, especially Outpatient use. Contents of the invention [0003] The invention provides a kit for the combined determination of aerobic bacteria and bacterial vaginosis and a preparation and use method. It has the characte...

Claims

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Application Information

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IPC IPC(8): C12Q1/56C12Q1/44C12Q1/34C12Q1/28C12Q1/14C12Q1/10C12Q1/04
Inventor 尹鹏
Owner 浙江亚培生物技术有限公司
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