Method for cultivating fritillaria przewalskii test-tube bulblet
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A technology of fritillaria and small bulbs in Gansu, applied in the field of agricultural biology, can solve the problems of low induction rate, cumbersome steps, and long cultivation period
Active Publication Date: 2015-10-07
平凉市青松中药饮片有限公司
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[0005] The purpose of the present invention is to provide a method for cultivating small bulbs of Fritillaria fritillaria in test tubes, so as to solve the problems of low induction rate, long cultivation period and cumbersome steps in the existing Fritillaria cultivation methods
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Embodiment 1-16
[0024] Examples 1-16: Induction of protocorms:
[0025] Take the squamous heart as the explant, inoculate it in the following medium after disinfection, and incubate in the basal medium (1 / 2MS, sucrose 50g / L, activated carbon 3g / L, agar 6g / L, refined Amino acid 1000mg / L) and different hormones were added for cultivation. See Table 1 for the ratio of different hormones, and the pH value of the medium was 6.0.
[0026]
[0027] Find out by the prescription of above embodiment, along with the increase of zeatin concentration, induction rate increases gradually, but 3mg / L of zeatin and 5mg / L difference are not big, and 5mg / L produces certain deformity; The increase of lactone, the induction rate difference is not big, but with the increase of brassinosteroid concentration, the transverse diameter of the protocorm tends to increase, but when the concentration of brassinosteroid reaches 0.5mg / L, the speed of induction initiation There is a tendency to slow down; with the inc...
Embodiment 16-25
[0028] Examples 16-25: Induction of protocorms:
[0029] Scale petals were used as explants, inoculated in the following medium after disinfection, and in the dark at 22°C in the basal medium (1 / 2MS, 50g / L sucrose, 3g / L activated carbon, 6g / L agar, refined Amino acid 1000mg / L) and different hormones were added for cultivation. See Table 2 for the ratio of different hormones, and the pH value of the medium was 6.5.
[0030]
Embodiment 26
[0031] Embodiment 26, induction of protocorms: the difference from Example 2 is:
[0032] The squamous heart was used as the explant, and cultured at 25°C under dark condition, the pH value of the medium was 5.5.
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Abstract
The invention discloses a method for cultivating fritillaria przewalskii test-tube bulblet and aims to solve the problems of low induction rate, long cultivation cycle and complicated steps of a conventional fritillaria cultivation method. The method comprises the following steps: selecting an explant to break fritillaria into cuiller and scale folus, sterilizing the explant, and then inducing the explant. The specific inducing method comprises the following steps: inoculating the sterilized explant into an inducing medium, cultivating under the dark condition that the temperature is 16 to 25 DEG C, and inducing protocorm at the base of the scale folus or the cuiller, wherein the inducing medium for the scale folus comprises the following formula: 50 to 80g/L of 1/2MS basic culture solution and sucrose, 2 to 5g/L of activated carbon, 5 to 6g/L of agar, 500 to 2,000mg/L of arginine, 1 to 5mg/L of zeatin (ZT), 0.1 to 0.5mg/L of brassinolide (BR) and 1 to 5mg/L of jasmonic acid methyl ester (MEJA), the hormone formula for the cuiller is different from that for the scale folus, and the pH value of the medium is 5.5 to 6.5. The method has the characteristics of high breeding speed, short cultivation cycle, high propagation rate and convenient transplantation, can be used for cultivating seed bulb, and can be used as a commodity.
Description
technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a method for directly cultivating test-tube bulblets by means of cell engineering. Background technique [0002] Fritillaria przewalskii (Fritillaria przewalskii) is the dry bulb of Liliaceae Fritillaria, mainly produced in Gansu, Qinghai, Sichuan and other provinces, together with F. cirrhosa, F. unibracteata and Suosha Fritillaria (F. delavayi), commonly known as Chuan Fritillaria, is a perennial herb. [0003] Gansu Fritillaria is an early spring ephemeral plant with a plant height of 20-45 cm and a short growth period of 60-90 days for the aboveground part. It generally takes 4-6 years from seed germination to flowering and fruiting. Seeds have both morphological and physiological post-ripening effects, making it difficult to break dormancy, and the natural germination rate and reproductive efficiency are very low. [0004] Artificial cultivation is very di...
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