Rapid induction medium for tissue culture of Alhagi sparsifolia Shap. and preparation method of rapid induction medium

Abstract

The invention belongs to the technical field of plant culturing, and in particular relates to a rapid induction medium for tissue culture of Alhagi sparsifolia Shap. and a preparation method of the rapid induction medium. The rapid induction medium is prepared by adding a specially-made curing agent, brown sugar, 6-benzylaminopurine, 4-chlorine-IAA, tetracycline, active carbon, a plant extract and papaya juice into a 1 / 2MS culture medium. According to the rapid induction medium, tetracycline is added, so that obvious inhibition effect can be achieved on endophyte of an explant, meanwhile, an extract of aerial roots of ficus virens is matched with 4-chlorine-IAA to effectively balance growth of axillary buds, roots and stems of Alhagi sparsifolia Shap. and to play the obvious role of promoting germination of the axillary buds and development of the buds. When the induction medium provided by the invention is used to culture Alhagi sparsifolia Shap., the survival rate is 98%-99%, the infection rate is 1.7%-2.1%, the browning degree of the medium is 14%-17% lower than that of the MS culture medium, and the germinating time is shortened by 25%-35%.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a rapid induction medium for camel thorn tissue culture and a preparation method thereof. Background technique [0002] Camel thorn (scientific name: Alhagi sparsifolia Shap.) belongs to the family Fabaceae, deciduous herb, the main branches are thorny, the leaves are oblong, and the flowers are pink. It blooms in June and is the most prosperous in August. Each flower can be open for more than 20 days and bear pods. Racemes, root system generally up to 20 meters. It is a naturally growing drought-tolerant plant that absorbs underground water and nutrients from the depths of deserts and Gobi. Because the stem of this plant has small, hard green leaves in the shape of thorns, it is called camel thorn. It is a herbaceous plant and a Gobi Camel grass is the only grass that camels can eat to survive in beaches and deserts, so it is also called camel grass, and i...

AI Technical Summary

Problems solved by technology

Because of its special physiological characteristics, if the commonly used auxin indole acetic acid is added to the induction medium at a low concentration, it cannot quickly promote stem and leaf differentiation. The concentration of indoleacetic acid is too high, and the growth and development of the root system are inhibited, resulting in the hypoplasia of the root system of the tissue cultured camel thorn