Liver cancer detection marker EZH2 epitope amino acid sequence and use thereof

A technology of antigenic epitopes and markers, applied in the biological field, can solve problems such as low sensitivity and poor specificity, and achieve high sensitivity and high specificity effects

Inactive Publication Date: 2015-11-04
JILIN JINUO BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently reported autoantibody detection methods have low sensit

Method used

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  • Liver cancer detection marker EZH2 epitope amino acid sequence and use thereof
  • Liver cancer detection marker EZH2 epitope amino acid sequence and use thereof
  • Liver cancer detection marker EZH2 epitope amino acid sequence and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Kit preparation

[0027] 1 Reagents See Tab.2-7 for reagent preparation.

[0028]

[0029]

[0030]

[0031]

[0032]

[0033]

[0034] 2 operations

[0035] (1) Coating: The enzyme plate should be washed 3 times with washing buffer, the working antigen should be diluted to the working concentration with the coating solution, coated on the enzyme plate, and kept overnight at 4°C.

[0036] (2) Add glutamic acid: Wash 3 times with washing buffer, dilute glutamic acid with coating solution to a concentration of 100 μg / ml, 200 μl per well, incubate at 37°C or room temperature for 1 hour;

[0037](3) Add plasma and quality control control (primary antibody): Wash the ELISA plate 3 times with washing buffer, and dilute the plasma to an appropriate concentration with the coating solution, generally 1:100-1:500, 100 μl per well, Incubate at 37°C or room temperature for 1 hour;

[0038] (4) Secondary antibody incubation: wash 3 times with washing buffer, d...

Embodiment 2

[0042] Detection of EZH2 auto IgG antibody in patients with liver cancer

[0043] 1 Sample collection: In this study, 103 liver cancer samples confirmed by radiological examination and histological examination were selected from the Third Hospital of Jilin University and Provincial Cancer Hospital. All serum samples had not undergone any anti-cancer treatment before collection, and had comprehensive clinical data and information. At the same time, 121 healthy control samples were recruited. The possibility of liver cancer was ruled out by clinical interview and imaging examination. The healthy group and the liver cancer group were matched in gender and age and were comparable ( P >0.05)

[0044] 2 Detection results: EZH2 autoantibody expression level (Tab.8): There was a statistical difference between the liver cancer group and the healthy control group ( t =4.312, P <0.001).

[0045] ROC curve analysis: The area under the ROC curve for EZH2 auto-IgG antibody detection ...

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Abstract

The invention discloses a liver cancer detection marker EZH2 epitope amino acid sequence and a use thereof and belongs to the technical field of immunology. The liver cancer detection marker EZH2 epitope amino acid sequence is a liver cancer-related gene EZH2 antigen amino acid sequence. The EZH2 multipeptide antigen can detect a corresponding specific autoantibody in liver cancer patient blood and the autoantibody can be used as a liver cancer detection marker for assessment of a liver cancer generation risk factor and prognosis curative effects. The multipeptide antigen and its antibody can be used for preparation of liver cancer early stage diagnosis and prognosis prediction reagents and a targeting drug for treating liver cancer.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a polypeptide fragment with detection liver cancer marker EZH2 screened by bioinformatics simulation. Background technique [0002] Liver cancer is currently the second most common malignant tumor in my country in terms of morbidity and mortality. There are about 300,000 new cases of liver cancer every year, accounting for more than 50% of the world. Liver cancer develops rapidly and easily metastasizes, and is currently one of the most difficult malignant tumors with the worst prognosis. However, compared with other types of malignant tumors such as breast cancer, lung cancer, and prostate cancer, the molecular understanding of tumor markers related to the pathogenesis of liver cancer is still lacking. A large number of studies have shown that tumor-associated antigens in serum or plasma can induce the body to produce autoantibodies, and there are both tumor antigens an...

Claims

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Application Information

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IPC IPC(8): C07K14/47G01N33/574G01N33/68
CPCC07K14/47G01N33/57438G01N33/57484G01N33/6803
Inventor 王雷王爽
Owner JILIN JINUO BIOLOGICAL ENG CO LTD
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