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Nickel chelate immune-complex and preparation method and application thereof

An immune complex and chelation-type technology, applied in the medical field, can solve problems such as changes in circulating immune complex content, functional changes, and increased disease susceptibility

Inactive Publication Date: 2015-11-04
广东腾湃医疗股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But how does environmental pollution stimulate the body to significantly increase the CIC content? Is it caused by one or multiple pollutants? Whether the increase in CIC will cause damage to the body and increase the susceptibility of the body to diseases? all to be studied
As we all know, circulating immune complexes are proteins of special significance, and nickel can act on proteins in multiple systems throughout the body. Whether nickel can act on circulating immune complexes will change the content, activity and function of circulating immune complexes , there is still a lack of relevant research on these

Method used

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  • Nickel chelate immune-complex and preparation method and application thereof
  • Nickel chelate immune-complex and preparation method and application thereof
  • Nickel chelate immune-complex and preparation method and application thereof

Examples

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preparation example Construction

[0064] Wherein, the preparation method comprises the steps of:

[0065] A) the step of synthesizing nickel chelating type immune complex;

[0066] B) The step of purifying the nickel-chelating immune complex: using immunoaffinity chromatography to remove carrier proteins, specific antibodies and nickel ions not involved in the reaction in the nickel-chelating immune complex synthesized in step A).

[0067] Specifically, in the preparation method of the nickel chelated immune complex,

[0068] Described step A) concrete steps are as follows:

[0069] (1) prepare chelating agent: chelating agent is dissolved in solvent, is mixed with chelating agent solution;

[0070] (2) Prepare the carrier protein solution: add the carrier protein to the borate solution;

[0071] (3) Stirring overnight: adding the chelating agent solution in step (1) to the carrier protein solution in step (2), stirring and acting in a shaker for 2-30 hours to obtain a mixed solution;

[0072] (4) Dialysis...

Embodiment 1

[0165] The reagents used in this embodiment are as follows:

[0166] Borate buffer solution, its molar concentration is 0.01M, and this borate buffer solution of every 500ml is made as follows again: take by weighing 0.31g boric acid and be dissolved in 400ml ultrapure water, adjust pH to with the NaOH aqueous solution of 0.1M 9.0, set the volume to 500mL, that is;

[0167] Human serum albumin solution, the preparation method is as follows: take human serum albumin and dissolve it in the above-mentioned borate buffer solution with a molar concentration of 0.01M and a pH of 9.0, fully oscillate and dissolve, and prepare a hemoglobin solution of 1.0 mg / mL;

[0168] 5mmol / L EDTA+200mmol / LNaHCO 3 Solution, 5mmol / L EDTA+200mmol / LNaHCO per 1000ml 3 The solution is prepared by the following method: Take 1.86g EDTA 2H 2 O, 16.8g NaHCO 3 , add 900mL of ultrapure water, then adjust the pH to 8.0 with a NaOH aqueous solution with a molar concentration of 1.0M, then add ultrapure wate...

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Abstract

The invention discloses a nickel chelate immune-complex and its preparation method and application. The nickel chelate immune-complex is one of the following complexes: a complex formed by binding nickel ion to an immune-complex, or a complex formed by binding nickel ion to a carrier protein and an antibody specifically binding to the carrier protein; or a complex formed by binding nickel ion to immune globulin and binding the nickel ion bound to the immune globulin to carrier protein. The nickel chelate immune-complex can be used in detecting whether serum of people in a region contains the nickel chelate immune-complex and detecting content of the nickel chelate immune-complex so as to indirectly reflect nickel pollution degree of the region.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a nickel chelating immune complex and a preparation method and application thereof. Background technique [0002] Immune complexes (IC) are the product of antigen-antibody binding. According to the definition of the National Committee for Scientific and Technical Terms, IC refers to the complex formed by the combination of antibody and soluble antigen. It is divided into two categories, one is the fixed immune complexes in the tissue, and the other is the immune complexes that are free in the blood and become circulating immune complexes (circulating immune complexes, CIC). Immune complexes play an indispensable role in clearing and destroying various antigens, and the result of the body's immune response is the continuous production of immune complexes. When the number of antigens (or antibodies) in the body is slightly more than that of antibodies (or antigens), an immune c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00C07K1/34G01N33/564G01N33/68
Inventor 张积仁阳帆董欣敏吴婧蔡睿孙遥赵乙木
Owner 广东腾湃医疗股份有限公司
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