A method of callus induction and tissue culture seedling rapid propagation

A technique for inducing callus and fetus is applied in the field of plant biology, and can solve the problems of hindering the application of fetus tissue culture seedlings, low proliferation coefficient and high culture cost, and achieves protection of ecological environment diversity, simple operation and low production cost. Effect

Active Publication Date: 2017-03-08
广西华泰药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the research process of Zoumatai tissue culture, because Zoumatai is a woody plant, it is difficult to sterilize when taking the shoots as explants, but the germination of sterilized seeds on the medium to obtain sterile plants, with its There are problems such as low multiplication coefficient and long subculture time in the induction of clustered buds for bud propagation buds, resulting in high cost of cultivation, which seriously hinders the application of Zoumatai tissue culture seedlings in artificial cultivation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for induction of Zouma fetus callus and rapid propagation of tissue culture seedlings, comprising the following steps:

[0027] (1) On the ultra-clean workbench, sterilize the seeds of Zoumatai with 75% ethanol solution for 1 min, wash with sterile water once, and then use 0.1% mercury chloride (HgCl 2 ) solution was sterilized for 10 min, washed with sterile water for 5 times, placed on sterilized filter paper to absorb water, inserted into MS medium and cultured for 40 days, and the seeds germinated to obtain sterile plants; the stems of sterile plants The segments and shoots were cultured on the callus induction medium for 40 days, and the induction rate was 100%;

[0028] The callus induction medium is: MS+2,4-D0.5 mg / L+6-BA0.1 mg / L+sucrose 20 g / L+agar 5 g / L, pH value is 5.8;

[0029] (2) On the ultra-clean workbench, cut the callus induced by the stem segment and bud of Zouma fetus into 0.2 cm × 0.2 cm small pieces, insert it into the proliferation medium...

Embodiment 2

[0039] A method for induction of Zouma fetus callus and rapid propagation of tissue culture seedlings, comprising the following steps:

[0040] (1) On the ultra-clean workbench, sterilize the seeds of Zoumatai with 75% ethanol solution for 1 min, wash with sterile water once, and then use 0.1% mercury chloride (HgCl 2 ) solution was sterilized for 10 min, washed with sterile water for 5 times, placed on sterilized filter paper to absorb water, inserted into MS medium and cultured for 40 days, and the seeds germinated to obtain sterile plants; the stems of sterile plants The segments and shoots were cultured on the callus induction medium for 40 days, and the induction rate was 100%;

[0041]The callus induction medium is: MS+2,4-D0.5 mg / L+6-BA0.1 mg / L+sucrose 20 g / L+agar 5 g / L, pH value is 5.8;

[0042] (2) On the ultra-clean workbench, cut the callus induced by the stem segment and bud of Zouma fetus into 0.2 cm × 0.2 cm small pieces, insert it into the proliferation medium ...

Embodiment 3

[0052] A method for induction of Zouma fetus callus and rapid propagation of tissue culture seedlings, comprising the following steps:

[0053] (1) On the ultra-clean workbench, sterilize the seeds of Zoumatai with 75% ethanol solution for 1 min, wash with sterile water once, and then use 0.1% mercury chloride (HgCl 2 ) solution was sterilized for 10 min, washed with sterile water for 5 times, placed on sterilized filter paper to absorb water, inserted into MS medium and cultured for 40 days, and the seeds germinated to obtain sterile plants; the stems of sterile plants The segments and shoots were cultured on the callus induction medium for 40 days, and the induction rate was 100%;

[0054] The callus induction medium is: MS+2,4-D0.5 mg / L+6-BA0.1 mg / L+sucrose 20 g / L+agar 5 g / L, pH value is 5.8;

[0055] (2) On the ultra-clean workbench, cut the callus induced by the stem segment and bud of Zouma fetus into 0.2 cm × 0.2 cm small pieces, insert them into the proliferation medi...

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Abstract

The invention discloses a callus induction and tissue culture seedling rapid propagation method of rhizoma ardisiae gigantifoliae. According to the callus induction and tissue culture seedling rapid propagation method, rhizoma ardisiae gigantifoliae seeds are sterilely sowed to obtain sterile plants, and stems and buds of the sterile plants are subjected to callus induction; and a lot of buds can be rapidly obtained through a manner of differentiating calluses into the buds, so that the defect that the growth coefficients of cluster buds at a tissue culture phase of some plants are low is made up. With the adoption of the method disclosed by the invention, the quantity of the buds of rhizoma ardisiae gigantifoliae can be rapidly increased at the differentiating phase of the calluses, and a lot of rhizoma ardisiae gigantifoliae tissue culture seedlings can be obtained in short time, so that the culture cost is reduced and energy consumption is saved. The method disclosed by the invention is simple to operate and has low production cost, and can be used for producing germchits of rhizoma ardisiae gigantifoliae in a factory-like manner, so that a foundation is laid for sustainable utilization of rhizoma ardisiae gigantifoliae and protection of diversity of ecological environments.

Description

technical field [0001] The invention relates to the field of plant biology technology, in particular to a method for inducing callus of Zouma fetus and rapid propagation of tissue culture seedlings. Background technique [0002] Goofy ( Ardisia gigantifolia Stapf), also known as big-leaf Zijinniu, Zoumafeng, Doshou, is a plant of the genus Zijinniu in the family Zijinniu. The whole plant is used for medicine. There is a folk saying that "two feet can't move, and you can't leave the horse's womb". , carbuncle ulcer embolism. In recent years, with the in-depth research on the chemical composition and pharmacology of Zouma fetus, it also contains biologically active ingredients with significant anti-malignant, anti-thrombotic, anti-inflammatory, and anti-oxidative effects. The medicinal value of Zoumatai is constantly being explored, its application is becoming wider and wider, and the market demand is constantly increasing. [0003] Zoumatai is a small evergreen shrub with...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 石云平韦绍龙李小泉苏祖祥林茜张尚文
Owner 广西华泰药业有限公司
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