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Mink distemper-canine parvovirus enteritis bivalent vaccine and its preparation method and use

A technology for mink parvovirus and canine distemper virus, applied in the field of bioengineering, can solve the problem of no live mink canine distemper vaccine, etc., and achieve the effect of maintaining the immunogenicity of the virus

Inactive Publication Date: 2015-11-11
JL TEYAN BIOLOGICAL TECH LIMITED LIABILITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Based on the above two points, there is currently no dual vaccine for mink distemper live vaccine and mink parvovirus enteritis inactivated vaccine

Method used

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  • Mink distemper-canine parvovirus enteritis bivalent vaccine and its preparation method and use

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The preparation of embodiment 1 mink canine distemper live vaccine:

[0028] 1. Passaging and culturing of cells for seedling production: Digest Vero cells for producing mink distemper virus with trypsin-EDTA cell dispersion, and carry out passage at a ratio of 1:3; respectively add 8% to 10% calf serum The cell growth medium is cultured at 37°C, and when a good cell monolayer is formed, it is used for further subculture or virus inoculation.

[0029] 2. Propagation of cytotoxic species: Dilute mink canine distemper live vaccine CDV3-CL strain virulent species 3 to 5 times with cell maintenance fluid, and inoculate well-growing Vero monolayer cells at a ratio of 1% to 1.5%. Adsorb at 37°C for 1 hour, discard the adsorption solution, add cell growth medium and incubate at 37°C, when more than 80% of the cells show typical CPE, they can be harvested and used as virus seeds for production.

[0030] 3. Virus species identification: According to the appendix of "The Veterin...

Embodiment 2

[0051] The preparation of embodiment 2 mink parvovirus enteritis inactivated vaccine

[0052] 1. Passage and culture of cells for seedling production: Digest the CRFK cells used for producing mink parvovirus with trypsin-EDTA cell dispersion, and carry out passage at a ratio of 1:3; respectively add cells containing 8% to 10% calf serum for growth culture solution at 37°C for further passage or virus inoculation.

[0053] 2. Propagation of cytotoxic species: Properly dilute the mink parvovirus MEVB strain virus species with cell maintenance solution, inoculate them in CRFK cell suspension simultaneously at a ratio of 2%, and culture them at 37°C for 3 to 4 days, and the proportion is more than 80%. When the cells show typical CPE, they can be harvested and stored at -20°C as virus seeds for production.

[0054] 3. Virus species identification: According to the appendix of "The Veterinary Drug Code of the People's Republic of China", there should be no growth of bacteria, mold...

Embodiment 3

[0067] Example 3 Preparation of Mink Parvovirus Enteritis Inactivated Vaccine and Mink Canine Distemper Live Vaccine Dual Vaccine

[0068] 1. Take out the frozen live mink distemper vaccine and the refrigerated mink parvovirus inactivated vaccine and put them at room temperature, and shake the mink parvovirus inactivated vaccine evenly;

[0069] 2. According to the ratio of 1:1, extract corresponding milliliters of inactivated mink parvovirus enteritis vaccine and inject it into the live mink distemper vaccine bottle, mix well and inoculate animals; the vaccine should be immunized within two hours after mixing, and each mink should be vaccinated. 1.0mL.

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Abstract

The invention discloses a mink distemper-canine parvovirus enteritis bivalent vaccine and its preparation method and use and belongs to the technical field of bioengineering. The mink distemper-canine parvovirus enteritis bivalent vaccine mainly comprises a mink distemper live vaccine, a mink canine parvovirus enteritis inactivated vaccine, a vaccine adjuvant and a protective agent. Aiming at one object, a ratio of the mink distemper live vaccine to the mink canine parvovirus enteritis inactivated vaccine is 1: 1. The invention also provides a preparation method and a use method of the mink distemper-canine parvovirus enteritis bivalent vaccine. The mink distemper-canine parvovirus enteritis bivalent vaccine is safe, has high efficiency, can save labor and can reduce a culture cost.

Description

technical field [0001] The invention relates to a mink canine distemper and parvoviral enteritis dual vaccine and a preparation method and application thereof, belonging to the technical field of bioengineering. Background technique [0002] Mink viral enteritis is an acute, severe and highly contagious infectious disease of mink caused by parvovirus. It is mainly characterized by severe diarrhea. The disease was discovered by Canadian scholar Schofield in 1949. Will proved that the pathogen of this disease is parvovirus in 1952. The virus has since spread to countries all over the world. In 1981, Jiang Tingxiu and others reported the occurrence of mink viral enteritis in my country; in 1984, Gao Yun et al reported the occurrence of mink viral enteritis in Shandong. Afterwards, the disease gradually spread throughout the country, bringing huge economic losses to the mink industry. [0003] Canine distemper is an acute, febrile, highly contagious highly contagious infectiou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/23A61K39/175A61P31/14A61P31/20
Inventor 罗国良彭风华陈立志程悦宁陈涛闫喜军赵建军谭斌
Owner JL TEYAN BIOLOGICAL TECH LIMITED LIABILITY
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