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Plant starch synthesized related protein IbSSI, coded gene and application thereof

A plant starch and coding technology, applied in the direction of plant gene improvement, application, plant peptides, etc., can solve the problem of difficulty in breeding new varieties of high-quality, high-yield, salt-tolerant and drought-resistant sweetpotatoes, restricting the utilization of sweetpotato breeding resources and free combination of parents, etc. question

Active Publication Date: 2015-11-18
CHINA AGRI UNIV
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AI Technical Summary

Problems solved by technology

[0003] Sweet potato is a vegetatively propagated crop. The incompatibility of interspecific and intraspecific hybridization severely limits the utilization of resources and the free combination of parents in sweet potato breeding. Breeding practice shows that it is difficult to breed high-quality, high-yielding, salt-tolerant plants with conventional cross-breeding methods. New drought-resistant sweet potato varieties

Method used

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  • Plant starch synthesized related protein IbSSI, coded gene and application thereof
  • Plant starch synthesized related protein IbSSI, coded gene and application thereof
  • Plant starch synthesized related protein IbSSI, coded gene and application thereof

Examples

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Effect test

Embodiment 1

[0064] Example 1. Obtaining and Identification of Transgenic Sweet Potatoes with Changes in Total Starch Content and Starch Quality

[0065] 1. Acquisition of IbSSI gene

[0066] The steps of obtaining the IbSSI gene are as follows:

[0067] 1. Acquisition of the template: Extract the total RNA of the young leaves of Xu 781 with a plant total RNA extraction kit (product of Tiangen Biochemical Technology (Beijing) Co., Ltd., catalog number DP432), and use the total RNA with PrimeScript TM 1stStrandcDNASynthesisKit (Bao Biological Engineering (Dalian) Co., Ltd.) reverse transcribed the first strand cDNA.

[0068] 2. Synthesize degenerate primers DS-F and DS-R.

[0069] 3. After completing steps 1 and 2, use the cDNA obtained in step 1 as a template, and use the DS-F and DS-R synthesized in step 2 as primers to obtain EST sequence 1 of about 652 bp by PCR amplification and sequence. According to the sequence of EST sequence 1, primers 3GSP1 and 3GSP2 were designed and synthesi...

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Abstract

The invention discloses a plant starch synthesized related protein IbSSI, a coded gene and application thereof. The plant starch synthesized related protein IbSSI provided by the invention is 1) or 2) or 3): 1) the protein with an amino acid sequence shown as sequence 2 of a sequence table; 2) fusion protein obtained by connecting a label to the N or / and C end of the protein shown as the sequence 2 of the sequence table; and 3) the plant starch synthesis related protein obtained by subjecting the protein shown as 1) or 2) to substitution and / or deletion and / or adding by one or more amino acid residues. Experiments prove that the plant starch synthesized related protein IbSSI and the coded gene thereof provided by the invention can increase the total content of plant starch and / or change the starch quality.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a plant starch synthesis-related protein IbSSI and its coding gene and application. Background technique [0002] Sweet potato (Ipomoeabatatas (L.) Lam.) is an important food, feed and industrial raw material crop, and as a new energy plant in the world today, its status is particularly important. my country is the world's largest sweet potato producer, with an annual planting area of ​​3.482 million hectares, accounting for 43.0% of the world's total planting area, and its annual output accounts for 68.6% of the world's total output. my country is currently in a period of rapid development, and the demand for energy continues to increase, so finding new alternative energy sources will be a long-term strategic work. As a root crop, sweet potato is rich in starch, and starch is one of the important raw materials for industrial ethanol production. Therefore, through in-dep...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N1/21C12N5/10C12N15/82A01H5/00
CPCC07K14/415C12N15/8245
Inventor 何绍贞王雁楠刘庆昌翟红李仁崑
Owner CHINA AGRI UNIV
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