An integrated dual-enzyme-quantum dot preparation method for chemiluminescence resonance energy transfer

A technology of resonance energy transfer and chemiluminescence, which is applied in the field of biological analysis, can solve problems affecting the simultaneous detection of multiple components, background spectrum interference, and reduce fluorescence efficiency, so as to reduce fluorescence quenching, design diversification, and reduce chemiluminescence background effect

Inactive Publication Date: 2017-06-20
CHENGDU UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these chemiluminescent systems generally need to add oxidizing agents such as H 2 o 2 , will oxidize CdSe, ZnS and other quantum dots to cause defects on their surface, thereby reducing their fluorescence efficiency or even quenching; When the quantum dot probe with short fluorescent wavelength is detected, it will cause serious background spectrum interference, which will affect the simultaneous detection of multiple components

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 (1) Preparation of sodium tellurium hydride: 60 mg tellurium powder, 120 mg sodium borohydride (NaHB 4 ) and 4 mL of 50 ℃ ultrapure water were reacted in a 10 mL centrifuge tube, and the tellurium powder was completely dissolved in the solution for 10 min.

[0015] (2) CdTe quantum dots of different sizes were prepared by hydrothermal synthesis: 200 mL10 mmol / L CdCl 2 Solution and 0.5712 g N-acetyl-L-cysteine ​​(NAC) were mixed uniformly in a three-necked flask, and the 2 Under the atmosphere, the reaction was stirred for 30 min, and the pH was adjusted to 8.0. Use a pipette gun to take 4 mL of the stock solution in (1) into a three-neck flask, stir for 10 min, and Cd 2 + / Te 2- The molar ratio of / NAC is 4:1:7. The reacted solution is poured into a hydrothermal reaction kettle, the reaction temperature is controlled to be 150°C, and the reaction time is 0.5-2 h to obtain a 500-650 nm aqueous solution of cadmium telluride quantum dots.

[0016] (3) Prepa...

Embodiment 2

[0017] Example 2 (1) Preparation of sodium tellurium hydride: 60 mg tellurium powder, 120 mg sodium borohydride (NaHB 4 ) and 4 mL of 50°C ultrapure water were reacted in a 10 mL centrifuge tube, and the tellurium powder was completely dissolved in the solution for 10 min.

[0018] (2) CdTe quantum dots of different sizes were prepared by hydrothermal synthesis: 200 mL10 mmol CdCl 2 Solution and 0.5712 g N-acetyl-L-cysteine ​​(NAC) were mixed uniformly in a three-necked flask, and the 2 Under the atmosphere, the reaction was stirred for 30 min, and the pH was adjusted to 8.0. Use a pipette gun to take 4 mL of the stock solution in (1) into a three-neck flask, stir for 10 min, and Cd 2+ / Te 2- The molar ratio of / NAC is 4:1:7. The reacted solution is poured into a hydrothermal reaction kettle, the reaction temperature is controlled to be 150° C., and the reaction time is 0.5-2 h to obtain a 500-650 nm aqueous solution of cadmium telluride quantum dots.

[0019] (3) Prepara...

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PUM

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Abstract

The invention relates to a preparation method for integrated bienzyme-quantum dots used for chemiluminescent resonance energy transfer. The integrated nanomaterial is composed of fluorescent quantum dots, peroxidase, and oxidase, with the fluorescent quantum dots being a core, and the peroxidase and the oxidase are successively connected to the quantum dots covalently. The integrated bienzyme-quantum dot nanomaterial is good in stability and water solubility, and the emission wavelength thereof can be adjusted. When the material is used for chemiluminescent resonance energy transfer, transfer efficiency is high (close to 50%), and is obviously higher than that of a chemiluminescent resonance energy transfer system with free bienzyme-quantum dots. The integrated bienzyme-quantum dots can be used to specifically detect substrate molecules corresponding to oxidase, multivariate analysis, cell or tissue imaging, and so on.

Description

technical field [0001] The invention relates to an integrated double enzyme-quantum dot preparation method for chemiluminescence resonance energy transfer, which is mainly used for chemiluminescence-quantum dot resonance energy transfer to detect biomolecules and perform imaging of cells and tissues, and belongs to the field of biological analysis. Background technique [0002] Quantum dots are usually spherical or spherical semiconductor nanoparticles with a diameter of 1-10 nm, which are composed of IIB~VIA or IIIA~VA elements. Due to its small particle size, electrons and holes are quantum-confined, and the continuous energy band becomes a discrete energy structure with molecular characteristics. When excited by a certain excitation light source, it will emit fluorescence. With the development of life sciences and the pursuit of human health, the application of quantum dot nanoprobes in nanotechnology has become a hot spot of concern. Compared with traditional dyes, quan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/88G01N21/76B82Y20/00B82Y40/00
Inventor 许淑霞李家林张信凤李显明
Owner CHENGDU UNIVERSITY OF TECHNOLOGY
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