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Preparation method for integrated bienzyme-quantum dots used for chemiluminescent resonance energy transfer

A resonance energy transfer and chemiluminescence technology, applied in the field of biological analysis, can solve the problems of simultaneous detection of multiple components, interference of background spectrum, reduction of fluorescence efficiency, etc., to reduce fluorescence quenching effect, design diversification, and reduce chemiluminescence background effect

Inactive Publication Date: 2015-11-18
CHENGDU UNIVERSITY OF TECHNOLOGY
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Problems solved by technology

However, these chemiluminescent systems generally need to add oxidizing agents such as H 2 o 2 , will oxidize CdSe, ZnS and other quantum dots to cause defects on their surface, thereby reducing their fluorescence efficiency or even quenching; When the quantum dot probe with fluorescent wavelength is detected, it will cause serious background spectrum interference, which will affect the simultaneous detection of multiple components

Method used

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Examples

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Embodiment Construction

[0013] specific implementation plan

[0014] Example 1 (1) Preparation of sodium telluride hydride: 60mg tellurium powder, 120mg sodium borohydride (NaHB 4 ) and 4mL of 50°C ultrapure water were reacted in a 10mL centrifuge tube, and the tellurium powder was completely dissolved in the solution for 10 minutes.

[0015] (2) CdTe Quantum Dots of Different Sizes Prepared by Hydrothermal Synthesis : 200mL10mmol / LCdCl 2 Solution and 0.5712g N-acetyl-L-cysteine ​​(NAC) were mixed uniformly in a three-necked flask, and the 2 Under the atmosphere, the reaction was stirred for 30 min, and the pH was adjusted to 8.0. Use a pipette gun to take 4mL of the stock solution in (1) into a three-necked flask, stir and react for 10min, Cd 2+ / Te 2- The molar ratio of / NAC is 4:1:7. The reacted solution is poured into a hydrothermal reaction kettle, the reaction temperature is controlled to be 150°C, and the reaction time is 0.5-2h to obtain a 500-650nm cadmium telluride quantum dot aqu...

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Abstract

The invention relates to a preparation method for integrated bienzyme-quantum dots used for chemiluminescent resonance energy transfer. The integrated nanomaterial is composed of fluorescent quantum dots, peroxidase, and oxidase, with the fluorescent quantum dots being a core, and the peroxidase and the oxidase are successively connected to the quantum dots covalently. The integrated bienzyme-quantum dot nanomaterial is good in stability and water solubility, and the emission wavelength thereof can be adjusted. When the material is used for chemiluminescent resonance energy transfer, transfer efficiency is high (close to 50%), and is obviously higher than that of a chemiluminescent resonance energy transfer system with free bienzyme-quantum dots. The integrated bienzyme-quantum dots can be used to specifically detect substrate molecules corresponding to oxidase, multivariate analysis, cell or tissue imaging, and so on.

Description

technical field [0001] The invention relates to an integrated double enzyme-quantum dot preparation method for chemiluminescence resonance energy transfer, which is mainly used for chemiluminescence-quantum dot resonance energy transfer to detect biomolecules and perform imaging of cells and tissues, and belongs to the field of biological analysis. Background technique [0002] Quantum dots are usually spherical or spherical semiconductor nanoparticles with a diameter of 1-10 nm, which are composed of IIB~VIA or IIIA~VA elements. Due to its small particle size, electrons and holes are quantum-confined, and the continuous energy band becomes a discrete energy structure with molecular characteristics. When excited by a certain excitation light source, it will emit fluorescence. With the development of life sciences and the pursuit of human health, the application of quantum dot nanoprobes in nanotechnology has become a hot spot of concern. Compared with traditional dyes, quan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/88G01N21/76B82Y20/00B82Y40/00
Inventor 许淑霞李家林张信凤李显明
Owner CHENGDU UNIVERSITY OF TECHNOLOGY
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