Inducing liquid for improving osteogenic differentiation efficiency in stem cells and application of inducing liquid

A technology for osteogenic differentiation and induction liquid, which is applied in the fields of nanomedicine and regenerative medicine to achieve the effects of good biocompatibility, improved differentiation efficiency and easy preparation

Active Publication Date: 2015-11-18
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the search found that there are no reports or patents on graphene quantum dots promoting osteogenic differentiation of stem cells and using them to prepare induction fluids that improve the efficiency of stem cell osteogenic differentiation.

Method used

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  • Inducing liquid for improving osteogenic differentiation efficiency in stem cells and application of inducing liquid
  • Inducing liquid for improving osteogenic differentiation efficiency in stem cells and application of inducing liquid
  • Inducing liquid for improving osteogenic differentiation efficiency in stem cells and application of inducing liquid

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Embodiment 1

[0021] Embodiment 1: preparation of graphene quantum dots

[0022] Reflux 0.5g pyrene in 40ml of nitric acid at 80°C for 12h, filter and wash with water, dissolve in 0.4M ammonia water and 1.5M hydrazine solution, put the solution at 200°C for hydrothermal reaction for 10h, dialyze with 0.22μm The graphene quantum dots are obtained after being filtered by a filter.

Embodiment 2

[0023] Example 2: Preparation of Inducing Solution for Improving Osteogenic Differentiation Efficiency of Stem Cells according to the present invention

[0024] In 1L of commercially available low-sugar DMEM medium, add 10% fetal bovine serum, 10mmol / L β-sodium glycerophosphate, 50mg / L ascorbic acid, 10nmol / L dexamethasone, and graphene at a final concentration of 10-30μg / mL by volume. Quantum dots, that is, to obtain an induction solution that improves the osteogenic differentiation efficiency of stem cells.

Embodiment 3

[0025] Example 3: The application of the induction solution for improving the osteogenic differentiation efficiency of stem cells according to the present invention in promoting the differentiation of stem cells into osteoblasts

[0026] Bone marrow mesenchymal cells were extracted and cultured in low-sugar DMEM medium containing 10% fetal bovine serum, 100 U / mL penicillin and 100 g / mL streptomycin at 37°C, 5% CO 2 cultured in an incubator.

[0027] After the above-mentioned bone marrow mesenchymal cells grow to the logarithmic growth phase and have more than 85% fusion, replace the induction solution for improving the osteogenic differentiation efficiency of stem cells described in the present invention, and change the induction solution containing graphene quantum dots every two days. liquid.

[0028] Cultured for 14-15 days, the marker protein alkaline phosphatase, marker genes OCN, Runx2 and marker mineralized nodules in the process of osteogenic differentiation were dete...

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Abstract

The invention discloses an inducing liquid for improving osteogenic differentiation efficiency in stem cells. The inducing liquid uses commercial low-sugar DMEM medium (dulbecco modified eagle medium) as an application matrix, wherein the inducing liquid further contains graphene quantum dot which is 0.1-100ug / mL in final concentration. The inducing liquid disclosed by the invention can be used for significantly improving osteogenic differentiation efficiency in stem cells; and the inducing liquid can be applied to bone tissue regeneration and repair in stem cell treatment, so as to promote directional differentiation; therefore, a function of regulating and controlling stem cell differentiation is achieved.

Description

technical field [0001] The invention belongs to the fields of nanometer medicine and regenerative medicine. The invention relates to an induction solution for improving the differentiation efficiency of stem cells to osteoblasts and its application. Background technique [0002] Stem cells are ideal seed cells for repairing damaged tissues due to their ability of self-renewal and multidirectional differentiation. Directed differentiation of stem cells can provide new technical methods for clinical treatment of tissue diseases and tissue defects. However, stem cells can not only differentiate into required tissue cells and participate in tissue repair, but also differentiate into other types of cells, and even have high tumorigenicity. Therefore, it is necessary to regulate the differentiation of stem cells so that they can differentiate into desired tissue cells or precursor cells. Improving the efficiency of directed differentiation and reducing the ability of stem cells...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077
Inventor 刘宏仇吉川桑元华马保金张珊梁龙跃
Owner SHANDONG UNIV
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