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A kind of inducing liquid and its application for improving the osteogenic differentiation efficiency of stem cells

A technology of osteogenic differentiation and induction solution, which is applied in the field of nanomedicine and regenerative medicine, and achieves the effect of simple and convenient use, wide range of sources and good biocompatibility

Active Publication Date: 2018-01-19
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the search found that there are no reports or patents on graphene quantum dots promoting osteogenic differentiation of stem cells and using them to prepare induction fluids that improve the efficiency of stem cell osteogenic differentiation.

Method used

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  • A kind of inducing liquid and its application for improving the osteogenic differentiation efficiency of stem cells
  • A kind of inducing liquid and its application for improving the osteogenic differentiation efficiency of stem cells
  • A kind of inducing liquid and its application for improving the osteogenic differentiation efficiency of stem cells

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Embodiment 1

[0021] Embodiment 1: preparation of graphene quantum dots

[0022] Reflux 0.5g pyrene in 40ml of nitric acid at 80°C for 12h, filter and wash with water, dissolve in 0.4M ammonia water and 1.5M hydrazine solution, put the solution at 200°C for hydrothermal reaction for 10h, dialyze with 0.22μm The graphene quantum dots are obtained after being filtered by a filter.

Embodiment 2

[0023] Example 2: Preparation of Inducing Solution for Improving Osteogenic Differentiation Efficiency of Stem Cells according to the present invention

[0024] In 1L of commercially available low-sugar DMEM medium, add 10% fetal bovine serum, 10mmol / L β-sodium glycerophosphate, 50mg / L ascorbic acid, 10nmol / L dexamethasone, and graphene at a final concentration of 10-30μg / mL by volume. Quantum dots, that is, to obtain an induction solution that improves the osteogenic differentiation efficiency of stem cells.

Embodiment 3

[0025] Example 3: The application of the induction solution for improving the osteogenic differentiation efficiency of stem cells according to the present invention in promoting the differentiation of stem cells into osteoblasts

[0026] Bone marrow mesenchymal cells were extracted and cultured in low-sugar DMEM medium containing 10% fetal bovine serum, 100 U / mL penicillin and 100 g / mL streptomycin at 37°C, 5% CO 2 cultured in an incubator.

[0027] After the above-mentioned bone marrow mesenchymal cells grow to the logarithmic growth phase and have more than 85% fusion, replace the induction solution for improving the osteogenic differentiation efficiency of stem cells described in the present invention, and change the induction solution containing graphene quantum dots every two days. liquid.

[0028] Cultured for 14-15 days, the marker protein alkaline phosphatase, marker genes OCN, Runx2 and marker mineralized nodules in the process of osteogenic differentiation were dete...

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Abstract

The invention discloses an induction solution for improving the osteogenic differentiation efficiency of stem cells. The commercially available low-sugar DMEM medium is used as an application matrix, and the medium also contains graphene quantum dots with a final concentration of 0.1-100 μg / mL. The induction solution provided by the invention can significantly improve the osteogenic differentiation efficiency of stem cells, and can be used to treat bone tissue regeneration and repair with stem cells, enabling directional differentiation and regulating stem cell differentiation.

Description

technical field [0001] The invention belongs to the fields of nanometer medicine and regenerative medicine. The invention relates to an induction solution for improving the differentiation efficiency of stem cells to osteoblasts and its application. Background technique [0002] Stem cells are ideal seed cells for repairing damaged tissues due to their ability of self-renewal and multidirectional differentiation. Directed differentiation of stem cells can provide new technical methods for clinical treatment of tissue diseases and tissue defects. However, stem cells can not only differentiate into required tissue cells and participate in tissue repair, but also differentiate into other types of cells, and even have high tumorigenicity. Therefore, it is necessary to regulate the differentiation of stem cells so that they can differentiate into desired tissue cells or precursor cells. Improving the efficiency of directed differentiation and reducing the ability of stem cells...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/077
Inventor 刘宏仇吉川桑元华马保金张珊梁龙跃
Owner SHANDONG UNIV
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