Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Sealed portable nucleic acid detection apparatus and method thereof

A detection method and portable technology, applied in biochemical cleaning devices, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve problems such as cross-contamination, viscosity of reaction reagents, and increased reaction costs, and reduce pollution. , the detection cost is low, the versatility is good

Inactive Publication Date: 2015-11-18
ZHEJIANG UNIV +1
View PDF4 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And for amplification reactions such as recombinant polymerase amplification (RPA) and recombinase-mediated amplification (RAA), the reaction reagents are relatively viscous, which is not conducive to the detection of the amplification product through the siphon effect on the test strip. ; or such as cross-primer amplification (CPA), polymerase chain reaction (PCR), etc. Although the reagents are not so viscous as to affect the false negative test result of the test strip, the test strip usually requires a certain volume guarantee, For example, the solution volume is at least 50ul or 100ul, and a larger amplification volume often means an increase in the cost of the reaction, so effective dilution of the amplification product is usually used by researchers.
However, if the amplified product is directly uncapped and diluted, it is easy to cause cross-contamination

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Sealed portable nucleic acid detection apparatus and method thereof
  • Sealed portable nucleic acid detection apparatus and method thereof
  • Sealed portable nucleic acid detection apparatus and method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1, with reference to figure 1 .

[0032] A portable visual detection device that can be directly used as a nucleic acid amplification PCR tube or eight-tube cap or a cover of a 96-well PCR plate for visual nucleic acid amplification detection. The device includes a visual mixing container 2 with multiple A tube wall cavity 1 connected with PCR tubes or eight-tubes or 96-well plates is used as a connection port, and the visualization mixing container itself has a mouth and a plug 3 that is sealingly connected with the mouth.

[0033] The visual inspection device of the present invention can be connected to two, three, four... or even more pipes at the same time, and can be adjusted according to needs; A 96-well PCR plate or a 384-well PCR plate, etc., adjusted as needed. The part connected with the PCR tube or the eight-tube or the 96-well PCR plate can be sealed with the tube cover from the inner wall of the tube, and the tube cover can be firmly locked i...

Embodiment 21

[0039] Example 2.1, with reference to figure 2

[0040] A portable diluted test strip detection device for color development of test strips and detection of nucleic acid amplification that can be directly used as a PCR tube cover or an eight-tube cover or a 96-well plate cover. The device can be connected and sealed at the same time. PCR tubes or eight-tubes or 96-well PCR plates, etc., and connect the reaction tubes through the visual mixing container 2 and place test strips.

[0041] The side of the visual mixing container 2 has a plurality of tube wall cavities 1 connected with PCR tubes or eight-tubes or 96-well plates as connection ports, and the test strips are placed on the test strips connected to and communicated with the visual mixing container 2 Detection container 3.

[0042] The end of the test strip detection container 3 has an inlet and a cover 4 . The test strip can be put in from the entrance or from the connection port (tube wall cavity 1).

[0043] In...

Embodiment 22

[0044] Embodiment 2.2, with reference to image 3

[0045] In embodiment 2.1, the cross-section of the test strip detection container 3 is substantially the same as that of the visualization mixing container 2 . However, in this embodiment, the height of the strip-shaped test strip detection container 3 is smaller than that of the mixing container. For the convenience of mold opening, now test strip can be inserted from the mouth 5 of the mixing container 2 self facing the test paper strip detection container 3 in strip shape, and mouth 5 is blocked by stopper 6. In order to ensure that the test strip is in full contact with the reaction reagent, the absorbent pad of the test strip can preferably be properly inserted into the mixing container, and there is no absorption of the connection port 1 in the section where the mixing container is close to the test strip detection container 3. Zone, the cross-sectional size of the absorption zone is between the mixing container 2 a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention provides a portable visualized nucleic acid detection method and a sealed portable nucleic acid detection apparatus. The nucleic acid detection apparatus comprises a visualized mixing container, wherein the visualized mixing container is provided with a plurality of connection ports, such that the visualized mixing container can be used as the total sealing body for connecting the nucleic acid amplification reaction tube on the visualized mixing container and the detection reaction liquid storage container on the visualized mixing container, or can be adopted as the total sealing body for connecting the nucleic acid amplification reaction tube on the visualized mixing container and the dilution storage container on the visualized mixing container. According to the present invention, the nucleic acid isothermal amplification reaction can be performed without interference in the case of achievement of no reaction tube opening, the subsequent detection can be normally performed according to the sequence, and the apparatus and the method can be suitable for chromogenic assay and test paper strip assay.

Description

technical field [0001] The invention belongs to the field of nucleic acid detection, and in particular relates to a device and method which can be used for common polymerase chain reaction (PCR), constant temperature nucleic acid amplification and other detections. Background technique [0002] With the development of science and technology, nucleic acid testing has been increasingly used in medical diagnosis, food safety testing and other fields. At present, the commonly used nucleic acid amplification methods include the amplification technology based on the classic common polymerase chain reaction (PCR) and the emerging constant temperature amplification technology, such as the cross-primed constant temperature amplification technology (CPA), the loop-mediated constant temperature amplification technology ( LAMP), strand displacement amplification (SDA), helicase-dependent amplification (HDA), recombination polymerase amplification (RPA), recombinase-mediated amplificatio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68C12M1/34
CPCC12Q1/6844C12Q1/686
Inventor 吴坚王柳陆绍红孔庆明
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com