Sealed portable nucleic acid detection apparatus and method thereof

A detection method and portable technology, applied in biochemical cleaning devices, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve problems such as cross-contamination, viscosity of reaction reagents, and increased reaction costs, and reduce pollution. , the detection cost is low, the versatility is good

Inactive Publication Date: 2015-11-18
ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And for amplification reactions such as recombinant polymerase amplification (RPA) and recombinase-mediated amplification (RAA), the reaction reagents are relatively viscous, which is not conducive to the detection of the amplification product through the siphon effect on the test strip. ; or such as cross-primer amplification (CPA), polymerase chain reaction (PCR), etc. Although the reagents are not so viscous as to affect the false

Method used

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  • Sealed portable nucleic acid detection apparatus and method thereof
  • Sealed portable nucleic acid detection apparatus and method thereof
  • Sealed portable nucleic acid detection apparatus and method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0031] Example 1, reference figure 1 .

[0032] A portable visual detection device that can be directly used as a PCR tube for nucleic acid amplification, an eight-tube cover or a 96-well PCR plate cover and used for visual nucleic acid amplification detection. The device includes a visual mixing container 2 with multiple sides of the mixing container 2 A tube wall cavity 1 connected with a PCR tube, an eight-tube tube or a 96-well plate is used as a connection port, and the visualization mixing container itself has a mouth and a plug 3 sealedly connected to the mouth.

[0033] The visual detection device of the present invention can be connected to two, three, four... or even more tubes at the same time, and adjusted as required; the visual detection device of the present invention can also extend from multiple directions such as horizontal and vertical at the same time, and even cover the entire Block 96-well PCR plate or 384-well PCR plate, etc., adjust as needed. The part com...

Example Embodiment

[0039] Example 2.1, reference figure 2

[0040] A portable dilution type test strip detection device that can be directly used as a PCR tube cover, an eight-pipe tube cover or a 96-well plate cover for test strip color development and nucleic acid amplification. This device can be connected and sealed at the same time. PCR tubes or eight tubes or 96-well PCR plates, etc., and the reaction tubes are connected through the visual mixing container 2 and test strips are placed.

[0041] The side of the visualization mixing container 2 has a plurality of tube wall cavities 1 connected with PCR tubes or eight-tubes or 96-well plates as connection ports, and test strips are placed in the test strips connected to and communicated with the visualization mixing container 2 Detection container 3.

[0042] The test strip detection container 3 has an entrance and a lid 4 at its end. The test strip can be inserted from the entrance or the connection port (tube wall cavity 1).

[0043] In order t...

Example Embodiment

[0044] Example 2.2, reference image 3

[0045] In Example 2.1, the cross section of the test strip detection container 3 is basically the same as the visual mixing container 2. However, in this embodiment, the height of the strip-shaped test strip detection container 3 is smaller than the height of the mixing container. For the convenience of mold opening, at this time, the test strip can be inserted from the mouth 5 of the mixing container 2 itself facing the strip-shaped test strip detection container 3, and the mouth 5 is blocked by a plug 6. In order to ensure that the test strip is in full contact with the reaction reagents, the absorbent pad of the test strip can preferably enter the mixing container appropriately, and there is no absorption of the connecting port 1 in the setting section of the mixing container near the test strip detection container 3 Zone, the cross-sectional size of the absorption zone is between the mixing container 2 and the test strip detection co...

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Abstract

The present invention provides a portable visualized nucleic acid detection method and a sealed portable nucleic acid detection apparatus. The nucleic acid detection apparatus comprises a visualized mixing container, wherein the visualized mixing container is provided with a plurality of connection ports, such that the visualized mixing container can be used as the total sealing body for connecting the nucleic acid amplification reaction tube on the visualized mixing container and the detection reaction liquid storage container on the visualized mixing container, or can be adopted as the total sealing body for connecting the nucleic acid amplification reaction tube on the visualized mixing container and the dilution storage container on the visualized mixing container. According to the present invention, the nucleic acid isothermal amplification reaction can be performed without interference in the case of achievement of no reaction tube opening, the subsequent detection can be normally performed according to the sequence, and the apparatus and the method can be suitable for chromogenic assay and test paper strip assay.

Description

technical field [0001] The invention belongs to the field of nucleic acid detection, and in particular relates to a device and method which can be used for common polymerase chain reaction (PCR), constant temperature nucleic acid amplification and other detections. Background technique [0002] With the development of science and technology, nucleic acid testing has been increasingly used in medical diagnosis, food safety testing and other fields. At present, the commonly used nucleic acid amplification methods include the amplification technology based on the classic common polymerase chain reaction (PCR) and the emerging constant temperature amplification technology, such as the cross-primed constant temperature amplification technology (CPA), the loop-mediated constant temperature amplification technology ( LAMP), strand displacement amplification (SDA), helicase-dependent amplification (HDA), recombination polymerase amplification (RPA), recombinase-mediated amplificatio...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12M1/34
CPCC12Q1/6844C12Q1/686
Inventor 吴坚王柳陆绍红孔庆明
Owner ZHEJIANG UNIV
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