Method for promoting none-flowering-phase kiwi fruits to flower under in vitro condition
A technology of kiwifruit and conditions, applied in the agricultural field, can solve the problems of long innovation cycle of germplasm and cannot reflect the superiority of haploid to the maximum extent, and achieve the effect of wide application range.
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Embodiment 1
[0028] 1) Test steps:
[0029] In the experimental base of the Zhenjiang Institute of Agricultural Sciences in the hilly area of Jiangsu, the branches of Actinidia sinensis were selected for in vitro experiments. A specific method for promoting the flowering of kiwifruit in non-flowering stages under in vitro conditions includes the following steps:
[0030] (1) Branch selection and processing: Collect branches used as cuttings in November; collect annual lignified branches from male kiwifruit plants with good tree vigor, wrap them in wet newspaper and place them in a 0-4°C refrigerator for refrigeration; refrigerate The processing time is not less than 1 week. On the one hand, the accumulation of cooling capacity is carried out, and on the other hand, the isolated branches are preserved;
[0031] (2) Preparation of cutting bed: The cutting bed is built in a greenhouse in a sunny place. The soil at the bottom of the cutting bed is deeply turned and exposed to the sun and mad...
Embodiment 2
[0039] 1) Test steps:
[0040] In the experimental base of the Zhenjiang Institute of Agricultural Sciences in the hilly area of Jiangsu, branches of delicious kiwifruit were selected for in vitro experiments. A specific method for promoting flowering of kiwifruit in non-flowering stages under in vitro conditions includes the following steps:
[0041] (1) Branch selection and processing: Collect branches used as cuttings in November; collect annual lignified branches from delicious kiwifruit male plants with good tree vigor, wrap them in wet newspaper and place them in a refrigerator at 0-4°C for refrigeration; refrigerate The processing time is not less than 1 week. On the one hand, the accumulation of cooling capacity is carried out, and on the other hand, the isolated branches are preserved;
[0042] (2) Preparation of cutting bed: The cutting bed is built in a greenhouse in a sunny place. The soil at the bottom of the cutting bed is deeply turned and exposed to the sun a...
Embodiment 3
[0050] 1) Test steps:
[0051] In the experimental base of the Zhenjiang Institute of Agricultural Sciences in the hilly area of Jiangsu, kiwifruit branches were selected for in vitro experiments. Specifically, a method for promoting the flowering of kiwifruit in non-flowering stages under in vitro conditions includes the following steps:
[0052] (1) branch selection and processing: collect branches used as cuttings in November; collect annual lignified branches from male kiwifruit plants with good tree vigor, wrap them in wet newspaper and place them in a 0-4°C refrigerator for refrigeration; The refrigerated treatment time is not less than 1 week, on the one hand, the accumulation of cooling capacity is carried out, and on the other hand, the isolated branches are preserved;
[0053] (2) Preparation of the cutting bed: The cutting bed was built in a greenhouse in a sunny place. The soil at the bottom of the cutting bed was deeply plowed and exposed to the sun and made int...
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