A method for promoting flowering of non-flowering kiwifruit under in vitro conditions

Abstract

The invention discloses a method for promoting none-flowering-phase kiwi fruits to flower under the in vitro condition. The method comprises the steps that certain chilling requirements are accumulated for in vitro twigs, an appropriate external environmental condition is given, the twigs are made to grow and develop in an established mode, and the purpose of generating flower buds and flowering ahead of time is achieved. Compared with the prior art, the method has the advantages that the none-flowering-phase kiwi fruits can obtain the flower buds, and the germplasm innovation for kiwi fruit haploid (double haploid) can be conducted multiple times in one year; the application scope is wide, the method can be popularized to other woody fruit trees, and original experimental materials are provided for the germplasm innovation based on the haploid (double haploid) technology.

Description

technical field

[0001] The invention relates to the field of agricultural technology, in particular to a method for promoting flowering of kiwifruit in non-flowering stages under in vitro conditions. Background technique

[0002] Plant haploid (double haploid) technology is a fast and effective germplasm innovation method, which can be realized through the method of anther (microspore) culture, and is mostly used in economic crops such as Brassicaceae, Gramineae, and tobacco. It is rarely used in woody plants such as various fruit trees. As a kind of woody plant, fruit tree has a longer infancy (childhood) and blooms once a year after adulthood; it cannot be planted and flowered in multiple seasons like wheat and rice. This seriously hinders the innovation process of fruit tree germplasm based on haploid (double haploid) technology.

[0003] At present, haploid (double haploid) technology has basically not been carried out in kiwifruit germplasm innovation. There are curr...

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