A method for promoting flowering of non-flowering kiwifruit under in vitro conditions

Abstract

The invention discloses a method for promoting none-flowering-phase kiwi fruits to flower under the in vitro condition. The method comprises the steps that certain chilling requirements are accumulated for in vitro twigs, an appropriate external environmental condition is given, the twigs are made to grow and develop in an established mode, and the purpose of generating flower buds and flowering ahead of time is achieved. Compared with the prior art, the method has the advantages that the none-flowering-phase kiwi fruits can obtain the flower buds, and the germplasm innovation for kiwi fruit haploid (double haploid) can be conducted multiple times in one year; the application scope is wide, the method can be popularized to other woody fruit trees, and original experimental materials are provided for the germplasm innovation based on the haploid (double haploid) technology.

Description

technical field

[0001] The invention relates to the field of agricultural technology, in particular to a method for promoting flowering of kiwifruit in non-flowering stages under in vitro conditions. Background technique

[0002] Plant haploid (double haploid) technology is a fast and effective germplasm innovation method, which can be realized through the method of anther (microspore) culture, and is mostly used in economic crops such as Brassicaceae, Gramineae, and tobacco. It is rarely used in woody plants such as various fruit trees. As a kind of woody plant, fruit tree has a longer infancy (childhood) and blooms once a year after adulthood; it cannot be planted and flowered in multiple seasons like wheat and rice. This seriously hinders the innovation process of fruit tree germplasm based on haploid (double haploid) technology.

[0003] At present, haploid (double haploid) technology has basically not been carried out in kiwifruit germplasm innovation. There are curr...

Examples

Embodiment 1

[0028] 1) Test steps:

[0029] In the experimental base of the Zhenjiang Institute of Agricultural Sciences in the hilly area of ​​Jiangsu, the branches of Actinidia sinensis were selected for in vitro experiments. A specific method for promoting the flowering of kiwifruit in non-flowering stages under in vitro conditions includes the following steps:

[0030] (1) Branch selection and processing: Collect branches used as cuttings in November; collect annual lignified branches from male kiwifruit plants with good tree vigor, wrap them in wet newspaper and place them in a 0-4°C refrigerator for refrigeration; refrigerate The processing time is not less than 1 week. On the one hand, the accumulation of cooling capacity is carried out, and on the other hand, the isolated branches are preserved;

[0031] (2) Preparation of cutting bed: The cutting bed is built in a greenhouse in a sunny place. The soil at the bottom of the cutting bed is deeply turned and exposed to the sun and mad...

Embodiment 2

[0039] 1) Test steps:

[0040] In the experimental base of the Zhenjiang Institute of Agricultural Sciences in the hilly area of ​​Jiangsu, branches of delicious kiwifruit were selected for in vitro experiments. A specific method for promoting flowering of kiwifruit in non-flowering stages under in vitro conditions includes the following steps:

[0041] (1) Branch selection and processing: Collect branches used as cuttings in November; collect annual lignified branches from delicious kiwifruit male plants with good tree vigor, wrap them in wet newspaper and place them in a refrigerator at 0-4°C for refrigeration; refrigerate The processing time is not less than 1 week. On the one hand, the accumulation of cooling capacity is carried out, and on the other hand, the isolated branches are preserved;

[0042] (2) Preparation of cutting bed: The cutting bed is built in a greenhouse in a sunny place. The soil at the bottom of the cutting bed is deeply turned and exposed to the sun a...

Embodiment 3

[0050] 1) Test steps:

[0051] In the experimental base of the Zhenjiang Institute of Agricultural Sciences in the hilly area of ​​Jiangsu, kiwifruit branches were selected for in vitro experiments. Specifically, a method for promoting the flowering of kiwifruit in non-flowering stages under in vitro conditions includes the following steps:

[0052] (1) branch selection and processing: collect branches used as cuttings in November; collect annual lignified branches from male kiwifruit plants with good tree vigor, wrap them in wet newspaper and place them in a 0-4°C refrigerator for refrigeration; The refrigerated treatment time is not less than 1 week, on the one hand, the accumulation of cooling capacity is carried out, and on the other hand, the isolated branches are preserved;

[0053] (2) Preparation of the cutting bed: The cutting bed was built in a greenhouse in a sunny place. The soil at the bottom of the cutting bed was deeply plowed and exposed to the sun and made int...

technical field

[0001] The invention relates to the field of agricultural technology, in particular to a method for promoting flowering of kiwifruit in non-flowering stages under in vitro conditions. Background technique

[0002] Plant haploid (double haploid) technology is a fast and effective germplasm innovation method, which can be realized through the method of anther (microspore) culture, and is mostly used in economic crops such as Brassicaceae, Gramineae, and tobacco. It is rarely used in woody plants such as various fruit trees. As a kind of woody plant, fruit tree has a longer infancy (childhood) and blooms once a year after adulthood; it cannot be planted and flowered in multiple seasons like wheat and rice. This seriously hinders the innovation process of fruit tree germplasm based on haploid (double haploid) technology.

[0003] At present, haploid (double haploid) technology has basically not been carried out in kiwifruit germplasm innovation. There are curr...

Examples

Embodiment 1

[0028] 1) Test steps:

[0029] In the experimental base of the Zhenjiang Institute of Agricultural Sciences in the hilly area of ​​Jiangsu, the branches of Actinidia sinensis were selected for in vitro experiments. A specific method for promoting the flowering of kiwifruit in non-flowering stages under in vitro conditions includes the following steps:

[0030] (1) Branch selection and processing: Collect branches used as cuttings in November; collect annual lignified branches from male kiwifruit plants with good tree vigor, wrap them in wet newspaper and place them in a 0-4°C refrigerator for refrigeration; refrigerate The processing time is not less than 1 week. On the one hand, the accumulation of cooling capacity is carried out, and on the other hand, the isolated branches are preserved;

[0031] (2) Preparation of cutting bed: The cutting bed is built in a greenhouse in a sunny place. The soil at the bottom of the cutting bed is deeply turned and exposed to the sun and mad...

Embodiment 2

[0039] 1) Test steps:

[0040] In the experimental base of the Zhenjiang Institute of Agricultural Sciences in the hilly area of ​​Jiangsu, branches of delicious kiwifruit were selected for in vitro experiments. A specific method for promoting flowering of kiwifruit in non-flowering stages under in vitro conditions includes the following steps:

[0041] (1) Branch selection and processing: Collect branches used as cuttings in November; collect annual lignified branches from delicious kiwifruit male plants with good tree vigor, wrap them in wet newspaper and place them in a refrigerator at 0-4°C for refrigeration; refrigerate The processing time is not less than 1 week. On the one hand, the accumulation of cooling capacity is carried out, and on the other hand, the isolated branches are preserved;

[0042] (2) Preparation of cutting bed: The cutting bed is built in a greenhouse in a sunny place. The soil at the bottom of the cutting bed is deeply turned and exposed to the sun a...

Embodiment 3

[0050] 1) Test steps:

[0051] In the experimental base of the Zhenjiang Institute of Agricultural Sciences in the hilly area of ​​Jiangsu, kiwifruit branches were selected for in vitro experiments. Specifically, a method for promoting the flowering of kiwifruit in non-flowering stages under in vitro conditions includes the following steps:

[0052] (1) branch selection and processing: collect branches used as cuttings in November; collect annual lignified branches from male kiwifruit plants with good tree vigor, wrap them in wet newspaper and place them in a 0-4°C refrigerator for refrigeration; The refrigerated treatment time is not less than 1 week, on the one hand, the accumulation of cooling capacity is carried out, and on the other hand, the isolated branches are preserved;

[0053] (2) Preparation of the cutting bed: The cutting bed was built in a greenhouse in a sunny place. The soil at the bottom of the cutting bed was deeply plowed and exposed to the sun and made int...

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