Rapid propagation method for hedyotis corymbosa tissue culture seedlings

A technology of corymbose and tissue culture seedlings, which is applied in the field of plant propagation, can solve the problems of no artificial breeding, etc., and achieve the effects of fast propagation speed, strong clustered buds, and shortening the seedling breeding cycle

Inactive Publication Date: 2015-12-02
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the medicinal materials and seedlings of A. corymbosa mainly come from wild resources, and there are no reports on artificial breeding and artificial planting techniques.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] An example of the tissue culture rapid propagation method of Corymbus corymophyll of the present invention, comprises the following steps:

[0017] (1) Selection and disinfection of explants: Take the mature uncracked fruit of C. corymbosa as explants, soak in 2% (v / v) detergent solution for 5 minutes, and rinse with linear tap water for 15-30 minutes. 100 milliliters of 0.1% (v / v) mercuric chloride that added 2-3 drops of Tween-20 were sterilized for 8-10min, rinsed with sterile water for 3-5 times, and finally removed surface moisture with sterile filter paper to obtain explants, The sterile water is distilled water sterilized by high pressure,

[0018] (2) Seed germination to obtain sterile test-tube plantlets: Peel off the explants obtained in step (1) on the ultra-clean workbench, inoculate the seeds into MS medium, and culture at a temperature of 23-27°C and light intensity 1500lux, cultured for 30 days under the conditions of 8-10 hours / day light time, and obtai...

Embodiment 2

[0023] Another example of the tissue culture rapid propagation method of Corymbus corymophyll of the present invention comprises the following steps:

[0024] (1) Selection and disinfection of explants: Take the mature uncracked fruit of C. corymbosa as explants, soak in 2% (v / v) detergent solution for 5 minutes, and rinse with linear tap water for 15-30 minutes. 100 milliliters of 0.1% (v / v) mercuric chloride that added 2-3 drops of Tween-20 were sterilized for 8-10min, rinsed with sterile water for 3-5 times, and finally removed surface moisture with sterile filter paper to obtain explants, The sterile water is distilled water sterilized by high pressure,

[0025] (2) Seed germination to obtain sterile test-tube plantlets: Peel off the explants obtained in step (1) on the ultra-clean workbench, inoculate the seeds into MS medium, and culture at a temperature of 23-27°C and light intensity 1500lux, cultured for 30 days under the conditions of 8-10 hours / day light time, and o...

Embodiment 3

[0030] Another example of the tissue culture rapid propagation method of Corymbus corymophyll of the present invention comprises the following steps:

[0031] (1) Selection and disinfection of explants: Take the mature uncracked fruit of C. corymbosa as explants, soak in 2% (v / v) detergent solution for 5 minutes, and rinse with linear tap water for 15-30 minutes. 100 milliliters of 0.1% (v / v) mercuric chloride that added 2-3 drops of Tween-20 were sterilized for 8-10min, rinsed with sterile water for 3-5 times, and finally removed surface moisture with sterile filter paper to obtain explants, The sterile water is distilled water sterilized by high pressure,

[0032](2) Seed germination to obtain sterile test-tube plantlets: Peel off the explants obtained in step (1) on the ultra-clean workbench, inoculate the seeds into MS medium, and culture at a temperature of 23-27°C and light intensity 1500lux, cultured for 30 days under the conditions of 8-10 hours / day light time, and ob...

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Abstract

A rapid propagation method for hedyotis corymbosa tissue culture seedlings includes the following steps that 1, a non-cracking fruit of hedyotis corymbosa is used as an explant to be disinfected; 2, the disinfected explant is stripped off on a clean bench, seeds are inoculated to an MS culture medium and induced to sprout to obtain sterile test-tube seedlings; 3, the sterile test-tube seedlings are placed in an MS propagation culture medium and cultured for rapid propagation to obtain cluster buds; 4, the cluster buds are placed in an MS rooting culture medium and cultured to obtain complete seedlings with roots; 5, the complete seedlings with the roots are hardened and then transplanted in a seedbed to grow for a month, and then the complete seedlings are transplanted to a land. The propagation coefficient of the hedyotis corymbosa cluster buds obtained through the culture method reaches 20-30 times, the rooting rate of the obtained tissue culture seedlings reaches 98.5%, the survival rate of seedbed transplant reaches 95%, and the large-scale seedling culture for hedyotis corymbosa is effectively achieved.

Description

technical field [0001] The invention relates to a method for plant propagation, in particular to a rapid propagation method for tissue-cultured seedlings of Corymodium corymophyllum. Background technique [0002] Corymbus corymbosa, Latin scientific name Oldenlandia corymbosa L., also known as Hedyotis and Waterline Grass, is a plant of the genus Auricularia in the Rubiaceae family. It is distributed in various provinces in southern China and has a large yield. The dried whole herb is used as medicine, and the medicinal materials contain various Triterpenoids, of which ursolic acid has the highest content, is its main active ingredient. Pharmacological studies have found that ursolic acid has various pharmacological effects such as anti-tumor, anti-hepatitis, anti-virus, and antibacterial, and has important development and application value. At present, the medicinal materials and seedlings of A. corymbosa mainly come from wild resources, and there are no reports on artific...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor韦范韦坤华李林轩黄宝优缪剑华徐建平李旻辉
OwnerGUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS