A kind of rapid propagation method of tissue culture of tsunami
A technique for combining the columnar lotus and tissue culture, which is applied in the field of plant reproduction, can solve the problems of the yield of cracked and scattered seeds, the seeds are small, and it is difficult to realize large-scale cultivation, so as to shorten the breeding cycle of seedlings, increase the breeding speed, and improve the seedlings. The effect of reproduction coefficient
Inactive Publication Date: 2017-03-29
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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Problems solved by technology
Under natural conditions, Nasturtium albicans mainly relies on seeds for reproduction, but the seeds are small, and the characteristics of cracking and scattering after fruit ripening often result in low seed yields, making it difficult to achieve large-scale cultivation in production
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[0016] The technical solutions of the present invention will be further described below in conjunction with the examples.
[0017] The rapid propagation method of Tsarthia chinensis tissue culture of the present invention comprises the following steps:
[0018] (1) Selection and disinfection of explants: select the seeds of Trolla chinensis as explants, soak them in 2% aqueous solution of detergent for 5 minutes, wash them with linear tap water for 15-30 minutes, add 2-3 drops of Tween- 20 100 milliliters of 0.1% mercuric chloride were sterilized for 8-10 minutes, rinsed with sterile water for 3-5 times, and finally removed surface moisture with sterile filter paper to obtain explants, and the sterile water was distilled water sterilized by high pressure;
[0019] (2) Seed germination to obtain sterile test-tube plantlets: Inoculate the explants obtained in step (1) into MS medium, at a culture temperature of 23-27°C, with a light intensity of 1500 lux, and a light time of 12-...
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The invention provides a tissue culture and rapid propagation method for sinia rhodoleuca, which comprises the following steps: (1) taking sinia rhodoleuca seeds as explants, and sterilizing; (2) putting the sterilized explants into an MS induction medium to induce germination so as to obtain sterile plantlets; (3) putting the sterile plantlets into an MS proliferation medium to carry out rapid propagation culture on the plantlets so as to obtain multiple shoots; (4) putting the multiple shoots into an MS strong seedling medium to carry out strong seedling culture so as to obtain strong plants; (5) putting the strong plants into a 1 / 2 MS rooting medium to culture so as to obtain complete seedlings with roots; and (6) hardening the complete seedlings with roots, then transplanting to a seedbed to grow for one month, and then transplanting to the land for growing field crops. The seedlings obtained by the method provided by the invention are robust and have a high survival rate, a large amount of high-quality seedlings of sinia rhodoleuca can be provided in a short time, and the problem of large-scale culture of seedlings of sinia rhodoleuca is effectively solved.
Description
technical field [0001] The invention relates to a method for plant propagation, in particular to a rapid propagation method for tissue culture of nasturtium algae. Background technique [0002] Sauvagesia rhodoleuca (Diels) M.C.E., also known as Sauvagesia rhodoleuca (Diels) M.C.E., is a small deciduous shrub of the Ochnaceae family and is a unique single-species plant in my country. The rhizome of Hezhu Tunami is used as medicine, which has the effects of relieving itching and killing insects. Because the morphological characteristics of Nasturtium algae are very close to those of Wallacea in South America, its existence is of great scientific significance for further research on the flora, geographical distribution, occurrence and evolution of this family of plants. [0003] Hezhu Jinlianmu is mainly distributed in Jinxiu, Longsheng, Rongshui, Debao, Guangxi and Fengkai, Lianshan, Huaiji and other places in Guangdong. It often grows in middle and low mountainous areas at...
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IPC IPC(8): A01H4/00
Inventor 韦荣昌
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS