A kind of rapid propagation method of Utricula chrysalis

A fast technology of Uriella yellowi, applied in the field of plant propagation, to achieve the effects of shortening the seedling breeding cycle, uniform seedling quality, and fast propagation speed

Inactive Publication Date: 2017-11-07
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there is no tissue culture technique report for the yellow raccoon

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] An example of the rapid propagation method of U. algae of the present invention, comprises the following steps:

[0015] (1) Selection and disinfection of explants: Take the branches and leaves of U. algae as explants, soak them in 2v / v% detergent aqueous solution for 5min, rinse with linear tap water for 15-30min, add 2-3 drops of vomit 100 ml of 0.1v / v% mercuric chloride at a temperature of -20 was disinfected for 8-10 minutes, rinsed with sterile water for 3-5 times, and finally removed the surface moisture with sterile filter paper to obtain explants, wherein the sterile water was sterilized by high temperature and high pressure of distilled water,

[0016] (2) Obtaining sterile test tube seedlings The explants obtained in step (1) were inoculated into MS+1.0mg / L6-BA+0.5mg / LNAA medium on the ultra-clean workbench, and the culture temperature was 23-27°C , the light intensity is 1500 lux, and the light time is 8-10 hours / day for 30 days, and then sterile test tube s...

Embodiment 2

[0020] An example of the rapid propagation method of U. algae of the present invention, comprises the following steps:

[0021] (1) Selection and disinfection of explants: Take the branches and leaves of U. algae as explants, soak them in 2v / v% detergent aqueous solution for 5min, rinse with linear tap water for 15-30min, add 2-3 drops of vomit 100 ml of 0.1v / v% mercuric chloride at a temperature of -20 was disinfected for 8-10 minutes, rinsed with sterile water for 3-5 times, and finally removed the surface moisture with sterile filter paper to obtain explants, wherein the sterile water was sterilized by high temperature and high pressure of distilled water,

[0022] (2) Obtaining sterile test tube seedlings The explants obtained in step (1) were inoculated into MS+1.0mg / L6-BA+0.5mg / LNAA medium on the ultra-clean workbench, and the culture temperature was 23-27°C , the light intensity is 1500 lux, and the light time is 8-10 hours / day for 30 days, and then sterile test tube s...

Embodiment 3

[0026] An example of the rapid propagation method of U. algae of the present invention, comprises the following steps:

[0027] (1) Selection and disinfection of explants: Take the branches and leaves of U. algae as explants, soak them in 2v / v% detergent aqueous solution for 5min, rinse with linear tap water for 15-30min, add 2-3 drops of vomit 100 ml of 0.1v / v% mercuric chloride at a temperature of -20 was disinfected for 8-10 minutes, rinsed with sterile water for 3-5 times, and finally removed the surface moisture with sterile filter paper to obtain explants, wherein the sterile water was sterilized by high temperature and high pressure of distilled water,

[0028] (2) Obtaining sterile test tube seedlings The explants obtained in step (1) were inoculated into MS+1.0mg / L6-BA+0.5mg / LNAA medium on the ultra-clean workbench, and the culture temperature was 23-27°C , the light intensity is 1500 lux, and the light time is 8-10 hours / day for 30 days, and then sterile test tube s...

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PUM

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Abstract

A utricularia aurea rapid propagation method comprises the following steps: (1) taking utricularia aurea branches and leaves as explants for disinfection; (2) inoculating the disinfected explants onto a MS + 1.0mg / L6-BA + 0.5mg / LNAA medium for induction germination to obtain sterile test-tube plantlets; (3) placing the sterile test-tube plantlets individually into a propagation medium for rapid propagation culture of the test-tube plantlets to obtain a large number of test-tube plantlets; and (4) hardening the test-tube plantlets, and transplanting the test-tube plantlets into a water plant water cluster box. By the utricularia aurea rapid propagation method, the propagation coefficient of the utricularia aurea test-tube plantlets can reach 30-50 times, survival rate of the utricularia aurea test-tube plantlets transplanted to the water plant water cluster box reaches 95-98%, and the utricularia aurea scale breeding problem can be effectively solved.

Description

technical field [0001] The invention relates to a method for plant reproduction, in particular to a method for rapid reproduction of U. algae. Background technique [0002] Utricularia aurea Lour. is an annual herb of the Utricularaceae family. It grows mostly in paddy fields or shallow water in still ponds. Setting up three-dimensional greening or water surface greening on large water bodies can increase the landscape diversity of water bodies. But its cultivation is difficult, requiring clean water and no pollution. In order to prevent the extinction of the wild U. algae resources and ensure the sustainable utilization of the U. algae resources, it is necessary to carry out research on the seedling breeding technology of U. In the prior art, there is no report of the tissue culture technology of the yellow raccoon. SUMMARY OF THE INVENTION [0003] The purpose of the present invention is to provide a rapid propagation method of U. algae, which can effectively and qui...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): A01H4/00
CPCA01H4/005
Inventor李林轩韦坤华韦范缪剑华李翠韦莹王一诺肖东
OwnerGUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS