Leontopodium haplophylloides extract and application thereof

A technology of edelweiss and its extract, which is applied in the field of edelweiss extract and its preparation, and can solve the problems such as the report of the anti-aging effect of edelweiss and the use of anti-aging cosmetics, etc.

Active Publication Date: 2015-12-02
JALA GROUP CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] Based on the above literature, there is no report on the a

Method used

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  • Leontopodium haplophylloides extract and application thereof
  • Leontopodium haplophylloides extract and application thereof
  • Leontopodium haplophylloides extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The preparation of embodiment 1 edelweiss extract

[0038] Method I:

[0039] Take edelweiss dry powder 100g, ultrasonically extract 2 times with 70% ethanol 900ml-500W at room temperature, each time for 30min, the filtrate is combined to obtain about 1700ml, take the filtrate and evaporate to dryness, obtain edelweiss crude extract 5.13g;

[0040] Method II:

[0041] Take 100g of dry edelweiss powder, extract it twice at room temperature with 70% ethanol 900ml-500W ultrasonic extraction, each time for 30min, the filtrate is combined to obtain about 1700ml, the filtrate is decompressed and the solvent is recovered until there is no alcohol smell, and the AB-8 resin is passed through. 30% ethanol, 50% ethanol and 70% ethanol were eluted, and the fractions were collected respectively, about 400ml each, and evaporated to dryness to obtain 2.64g of Edelweiss edelweiss crude extract, 0.83g and 70% ethanol eluted parts respectively Ethanol elution fraction 0.59g.

Embodiment 2

[0042] The effect of embodiment 2 edelweiss extracts on human fibroblast proliferation

[0043] According to the 30% and 70% ethanol elution parts of edelweiss prepared in Example 1, use deionized water to prepare a 0.02% concentration solution, join in the human fibroblast culture medium, use deionized water without sample as Blank control. After culturing for 48 hours, stain the cells with the MTT method, measure the absorbance at 550nm with a microplate reader, and evaluate the proliferation effect on human fibroblasts. The proliferation rate of the blank control is 100%, and the proliferation rate is higher than 120%. Has a proliferative effect.

[0044] Table 1 The effect of each elution fraction of Edelweiss edelweiss on the proliferation of human fibroblasts

[0045]

[0046] It can be seen from Table 1 that the edelweiss extracts used in the present invention all have a promoting effect on the proliferation of human fibroblasts, and the 70% ethanol eluted part has...

Embodiment 3

[0047] Antioxidant activity detection of embodiment 3 edelweiss extract

[0048] With the edelweiss extract prepared in Example 1, use the DPPH method to measure its free radical scavenging activity. The determination procedure is: the edelweiss extract is prepared into an aqueous solution with deionized water respectively, pipette 2mL in a 10mL stoppered test tube, add 2mL DPPH ethanol solution (2 × 10 -4 mol / L), fully mix, stand at room temperature, measure the absorbance A of 517nm wavelength with a spectrophotometer after 30min 517 (with ethanol as a reference); simultaneously measure the absorbance A after mixing 2mLDPPH solution with 2mL ethanol c , and the absorbance A after mixing 2mL extract with 2mL ethanol solution 0 , measured in parallel three times, averaged, and calculated the free radical scavenging rate according to the following formula.

[0049] Free radical scavenging rate = (1-(A 517 -A 0 ) / A c )×100%.

[0050] The calculation results are shown in T...

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Abstract

The invention discloses a leontopodium haplophylloides extract and application thereof. A preparation method comprises the following steps of ultrasonically extracting leontopodium haplophylloides dry powder with ethanol solution of which the volume concentration is 50-80% once to twice for 0.2-1.5 hours every time; separating solid from liquid to obtain filtrate; and merging and drying the filtrate by distillation to obtain the leontopodium haplophylloides extract. Quality-to-volume ratio of the leontopodium haplophylloides dry powder to ethanol solution is (1:8)-(1:15) g/mL. The leontopodium haplophylloides extract has good effects of whitening skin and resisting ageing, can be used for the fields of cosmetics, health food and medicines, and particularly can be used in cosmetics.

Description

technical field [0001] The invention belongs to the field of natural product chemistry, and in particular relates to an edelweiss extract, a preparation method and application thereof. Background technique [0002] Whitening and anti-aging cosmetics have always been the pursuit and yearning of women, and they are also eternal topics in the beauty industry. In recent years, with the development of social economy and the progress of science and technology, people's demand for cosmetics has also increased. [0003] The color of the skin mainly depends on the sum of skin pigments, the main component of which is melanin. The human body can produce two types of melanin: eumelanin and pseudomelanin. Eumelanin is primarily responsible for the visible color of skin, hair and eyes. In general, eumelanin is genetically regulated, in addition to being stimulated by UV exposure, and the degree of hyperpigmentation increases with age. Based on the traditional concept of Chinese orient...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61P39/06A61P17/18A61K8/97A61Q19/00A61Q5/12A61Q19/08A61Q19/02
Inventor 章漳黄晓青方兆华李慧张佳伟陈明华
Owner JALA GROUP CORPORATION
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