Extraction method of lincomycin

A technology of lincomycin and extraction method, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of different solubility, difficulty in separating and purifying crystalline lincomycin, etc., and achieve the process flow The effect of applying, improving the final output and improving the utilization rate

Inactive Publication Date: 2015-12-02
霍进铭
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Problems solved by technology

However, lincomycin is an amphoteric substance, and its isoelectric point is about pH4-5. Under different pH conditions, its solubility is different. In particular, lincomycin has a large number of homologues and isomers. These impurities are very similar to lincomycin in nature, therefore, it is very difficult to separate, purify or even crystallize lincomycin in industry through such a simple process

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  • Extraction method of lincomycin

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Embodiment 1

[0024] The present invention comprises the following steps:

[0025] a. After adjusting the pH of the lincomycin fermentation broth to 3, filter it with a ceramic membrane with a membrane pore size of 0.05 μm, then wash the ceramic membrane with an acidic aqueous solution of pH 3.0, discard the filtrate, and then wash the ceramic with a hydrochloric acid aqueous solution with a pH of 9 film, add purified water to dissolve to obtain lincomycin aqueous solution;

[0026] b. Add the decolorizing agent to the lincomycin aqueous solution obtained in step a for decolorization. After the decolorization is completed and filtered, press it into the stock solution tank to obtain the decolorizing solution. The decolorizing agent is activated carbon;

[0027] c. Add saturated n-butanol to the column decolorization solution obtained in step b for analysis, control the analysis flow rate, detect the analysis solution titer at the outlet of the resin column, collect the analysis solution to ...

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Abstract

The invention discloses an extraction method of lincomycin. A lincomycin fermentation solution is filtered through a ceramic film with pore diameters ranging from 0.01 mu m to 0.1 mu m after the pH (potential of hydrogen) of the lincomycin fermentation solution is adjusted to 2.0-4.5, then an acid aqueous solution with the pH ranging from 2.0 to 4.0 is used for washing the ceramic film repeatedly, filter liquor is discarded, a hydrochloric acid aqueous solution with the pH ranging from 8 to 10 is used for washing the ceramic film repeatedly, purified water is added for dissolution, and a lincomcin aqueous solution is obtained. Clear filter liquor is obtained after filtration of the ceramic film, a destaining solution and a desorbed solution can be both used for production of the next batch of lincomycin, the utilization rate of raw materials can be increased, meanwhile, the ultimate yield of the next batch of products can be further increased, and the extraction method has the advantages that a precipitation method is simple and convenient and a technological process is applicable to large-scale production.

Description

technical field [0001] The invention relates to the technical field of lincomycin precipitation methods, in particular to a method for extracting lincomycin. Background technique [0002] Lincomycin is the first product of a new family of antibiotics called cyclic lipopeptides. It is a small Streptomyces ( Streptomyces parvus ) is a novel cyclolipopeptide antibiotic composed of thirteen amino acids extracted from the fermentation broth, in which ten amino acids form a ring structure, and decanoic acid and tryptophan are esterified outside the ring. Not only does it have a novel chemical structure, but its mode of action is also different from any approved antibiotic. It can destroy the function of bacterial cell membrane in many ways, thereby killing Gram-positive bacteria rapidly. In addition to its ability to act on most clinically relevant Gram-positive bacteria, lincomycin still has strong in vitro resistance to isolated strains such as methicillin, vancomycin and l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/16C07H1/06
Inventor 霍进铭
Owner 霍进铭
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