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Detection method for medicinal material Ardisia mamillata Hance or extract thereof

The technology of the red hair trotter and the detection method, which is applied in the field of medicine, can solve the problems such as the quantitative analysis method of liangu saponin B and the like, and achieve the effects of simple and fast measurement, good linear relationship, and reliable quality control method.

Inactive Publication Date: 2015-12-02
SOUTHWEST UNIVERSITY FOR NATIONALITIES
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Problems solved by technology

[0003] At present, the research hotspots of this genus are mainly focused on the chemical components and anti-tumor activities, and there is no quantitative analysis method for the saponin B. The medicinal material was extracted and separated to obtain the monomer of saponin B, and the purification and structural identification were carried out according to the technical guidelines for the research of reference substances

Method used

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  • Detection method for medicinal material Ardisia mamillata Hance or extract thereof
  • Detection method for medicinal material Ardisia mamillata Hance or extract thereof
  • Detection method for medicinal material Ardisia mamillata Hance or extract thereof

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Embodiment Construction

[0021] 1 Instruments, reagents and samples

[0022] (3) High performance liquid chromatography (Agilent 1100 series), including online vacuum degasser (G1322A), quaternary gradient pump (G1311A), automatic sampler (G1313A), column oven (G1316A), DAD detection (G1314A), Chemstation chromatographic workstation; AlltechELSD2000 evaporative light scattering detector; preparative high-performance liquid chromatography (Beckman Coulter BECHMAN125P, USA), including SYSTEMGOLD125PSolventModule and 166pDetector, etc.; Milli-Q ultrapure water device (USA Milipore Company); Microfuge18 desktop centrifuge (Beckman Coulter Co., Ltd., USA); KQ-500DE ultrasonic cleaning machine (Kunshan Ultrasonic Instrument Co., Ltd., power 300W, frequency 40kHz); BP-210D electronic analytical balance (Germany Doris, one hundred thousandth); filter device (Autoscience) and microporous membrane (0.45μm). Analytical column: WatersSymmetryC 18 (150mm×4.5mm, 5μm); Phenomenex GeminiC 18 (150mm×4.5mm, 5μm); Al...

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Abstract

The invention provides a quantitative analysis method for a medicinal material Ardisia mamillata Hance or extract thereof. The method employs high performance liquid chromatography-evaporation light scattering detection process (HPLC-ELSD) to determine the content of the active component ardisiacrispin B in the medicinal material Ardisia mamillata Hance or extract thereof, so the inherent quality of the medicinal material Ardisia mamillata Hance or extract thereof can be objectively and scientifically evaluated. The precision, stability, repeatability, sample recovery, tolerance and the like of the method all meet quantitative analysis requirements. The method is used for determining the contents of the medicinal material Ardisia mamillata Hance produced from different areas for the first time and provides a novel quality control and detection means for medicinal development of the component ardisiacrispin B.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a detection method of active ingredients in traditional Chinese medicines, in particular to an HPLC-ELSD detection method of saponin B in red-haired Zoumatai medicinal material or its extract. Background technique [0002] Red-haired Zoumatai is the dried whole herb of Ardisiama millata Hance, a plant belonging to the genus Purple Gold Bovine. It is not only a famous ornamental plant, but also a commonly used herbal medicine in my country. Its medicinal value has been recorded in "Compendium of Materia Medica", and it has miraculous effects on the treatment of hepatitis, rheumatism, and malnutrition in children. Modern pharmacological studies have shown that this product has good anti-tumor activity, and its main active ingredient is ardisiacrispin B, which exists in various plants of the same genus Cinnabar root and Aurantium, and its chemical name is Sycra Min A3-O-α-rhamnopyran...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 张琦伍红陈炼红饶开晴
Owner SOUTHWEST UNIVERSITY FOR NATIONALITIES
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