39 results about "Ardisia mamillata" patented technology

The invention pertains to the pharmaceutical technical field, which relates to a preparation method and the usage of ardisia mamillata B, the invention takes the whole trees or branches and leaves or roots and rhizomes of the ardisia mamillata as the medicines, the water, methanol water solution with an arbitrary proportion, ethanol water solution or organic solvent are used for extraction, the extract liquid is filtered and concentrated; the concentrate is prepared by recrystallization and refining after the independent or combined separation by macroporous resin, column chromatography(alumina or silica gel). The invention has the advantages that: the invention provides the preparation method of ardisia mamillata B which can be used for anti-tumor, anti-inflammation, analgesia, anti-virus (such as, influenza viruses, herpes viruses, hepatitis viruses, anti-retroviral viruses including HIV and so on), the cost is low, the process is stable, the yield is more than 1 percent of the dry herbs, the invention can obtain the ardisia mamillata B with high purity which has been removed a large amount of impurities, and the pharmacological activity is significant.

The invention discloses a traditional Chinese medicine composition for treating a bone fracture. The composition is prepared from the following 23 traditional Chinese medicines: tinospora sinensis merr, heterosmilax japonica, smalt, pegia sarmentosa, flemingia philippinensis, gynostemma pentaphylla, aituoduo (Chinese character), herba periliae, asclepiadaceae tylophora koi, sinoadina racemosa, herba chloranthi serrate, clerodendrum serratum var amplexifolium, rhinacanthus nasutus, blumea balsamifera, sambucus chinensis, girardinia diversifolia, red elecampane, ardisia mamillata hance, uncaria rhynchophylla, rhizoma smilacis glabrae, tongking pepper, drynaria total flavonoids and epipremnum pinnatum. The traditional Chinese medicine composition works together to achieve the efficacies of promoting reunion of fractured bones, promoting blood circulation to remove blood stasis, relieving swelling and pain, soothing the channels and activating collaterals, has significant curative effects on treatment of the bone fracture, and can also be applied to soft tissue injuries, ligament and joint strain injuries caused by falling, hitting, touching, knocking and the like; and the symptoms such as local pain, tumidness, dysfunction, black and blue subcutaneous addiction spots or hematoma of patients are removed.

The invention discloses a traditional Chinese medicinal composition for treating traumatic injury. The composition consists of 20 traditional Chinese medicines, namely tinospora sinensis merr, heterosmilax japonica kunth, clerodendron cyrtophyllum, pegia sarmentosa, philippine flemingia root, gynostemma pentaphylla, munronia henryi harms, perilla leaf, dichidia tonkinensis costantin, adina racemosa, lysimachia paridiformis Franch, clerodendrum serratum var amplexifolium, rhinacanthus nasutus, blumea balsamifera, girardinia palmate, longpeduncle kadsura root, ardisia mamillata hance, uncaria rhynchophylla, rhizoma smilacis glabrae and piper boehmeriaefolium var. tonkinense. The traditional Chinese medicinal composition disclosed by the invention has functions of promoting blood circulation to remove stasis, relieving swelling and pain, unblocking channels and activating collaterals, and promoting reunion of fractured tendons and bones, and is applicable to soft tissue injury, tendon or joint strain and the like from falls, fractures, contusions, collisions or other reasons; and the traditional Chinese medicinal composition is convenient in using, rapid to take effect and significant in curative effect.

The invention discloses breakfast milk granules for relieving rheumatism. The breakfast milk granules are prepared from the following raw materials: skimmed milk, black rice, black sesame, soybean, black bean, coix seed, selenium-containing black peanut, walnut, chestnut, towel gourd, Chinese yam, purslane, pine pollen, menispermum dauricum, tinospora sinensis, leaf and twig of red psychotria, herba aristolochiae mollissimae, lysimachia paridiformis franch, ardisia mamillata hance, naringin, chlorophyll, a cedar essential oil, table salt, lactosucrose, direct vat set lactic acid bacteria, and kojic acid. The product is simple in preparation method, comprehensive in nutrition, mellow and strong in taste, fragrant, sweet and sour in flavor, and delicious; raw materials are abundant, are subjected to microbial fermentation and are easy to digest and absorb, the flavor is increased, and the shelf life is extended; the traditional Chinese medicines and health-care nutritional ingredients enrich nutrition and health care functions, can obviously relieve rheumatism, resist cancer, beautify face, care skin, promote gastrointestinal functions, resist aging, enhance immunity and prevent cardiovascular and cerebrovascular diseases; the breakfast milk granules do not contain any additives, are safe and healthy, are convenient to carry and eat, and can be directly eaten or be eaten after being brewed with water.

The invention discloses a preparation method for an ardisia mamillata triterpenoid saponin reference substance. The preparation method comprises the following steps: (1) performing extraction on the herb of ardisia mamillata; (2) performing macroporous adsorption resin column separation; (3) performing silica column chromatography; (4) performing preparative high performance liquid chromatography separation and purification, so as to obtain cyclamiretin A3-O-alpha-rhamnopyranoside-(1 to 2)-beta-D-glucopyranose-(1 to 4)-[beta-D-glucopyranose-(1 to 2)-]-alpha-L-arabinopyranoside. When the reference substance is detected by utilizing a plurality of development systems and developing systems of thin-layer chromatography, one spot is displayed in the chromatography; when the reference substance is further defected according to a high performance liquid chromatography-evaporative light scattering detection (HPLC-ELSD) method, the target peak of the reference substance is calculated according to a peak area normalization method, and the mass concentration of the reference substance is higher than 98%; when different chromatographic columns and a plurality of mobile phase systems are adopted for detection, the detection results are kept consistent and show that the reference substance, namely prepared cyclamiretin A3-O-alpha-rhamnopyranoside-(1 to 2)-beta-D-glucopyranose-(1 to 4)-[beta-D-glucopyranose-(1 to 2)-]-alpha-L-arabinopyranoside, meets the requirements of chemical reference substances for traditional Chinese medicine quantitative analysis. The preparation method adopts a safe and non-toxic extraction solvent, and is relatively high in finished product yield.

The invention provides a quantitative analysis method for a medicinal material Ardisia mamillata Hance or extract thereof. The method employs high performance liquid chromatography-evaporation light scattering detection process (HPLC-ELSD) to determine the content of the active component ardisiacrispin B in the medicinal material Ardisia mamillata Hance or extract thereof, so the inherent quality of the medicinal material Ardisia mamillata Hance or extract thereof can be objectively and scientifically evaluated. The precision, stability, repeatability, sample recovery, tolerance and the like of the method all meet quantitative analysis requirements. The method is used for determining the contents of the medicinal material Ardisia mamillata Hance produced from different areas for the first time and provides a novel quality control and detection means for medicinal development of the component ardisiacrispin B.

The invention relates to an explant disinfection method for culture of hair bearing plant tissue, and belongs to the technical field of plant tissue culture in the technical field of biotechnology. The explant disinfection method for culture of hair bearing plant tissue of the invention comprises: pre-culturing a mother plant; pre-treating the mother plant by using carbendazim; disinfecting the explant by using alcohol; then performing mercuric chloride based disinfection twice, wherein disinfection is performed by using mercuric chloride for 4 minutes, rinsing is performed five times by using sterile water, then disinfection is performed by using mercuric chloride for 4 minutes, and rinsing is performed five times by using sterile water; and then performing seeding in a start culture medium that contains yipeilong. The method can eliminate contamination bacteria on the surface of the ardisia mamillata explant, and keep the explant robust.

The invention belongs to the technical field of ardisia mamillata tissue culture, and particularly discloses an ex-vitro rooting method for ardisia mamillata tissue culture seedlings, which comprises the steps of seedling raising tray preparation, water preparation, matrix preparation, rooting agent preparation, bottle seedling preparation, cutting treatment, maintenance management, and root promoting and seedling strengthening. According to the adopted matrix and culture conditions, the pollution rate of the cuttings is almost 0, the cuttings start to root after being cultured for 15-20 days, the rooting rate reaches 100% after being cultured for 25-30 days, the average root length is 3.4 cm, the number of root points is 4.7, the root width is 4-5 cm, the average root length is 6.5 cm, the number of root points is 5.5, and the root width is 6-8 cm after being subjected to cuttage culture on a greenhouse seedbed for about 30 days, and seedlings with good root clusters are formed. The efficient rooting method is provided for ex-vitro rooting of the ardisia mamillata tissue culture seedlings, compared with in-bottle rooting, the root system quality is improved, the rooting time is shortened, the rooting procedure is optimized, the production cost is reduced, the method is simple, operability is high, and technical support can be provided for development of the whole industry chain of ardisia mamillata.

A traditional Chinese medicine composition for treating qi-failing-to-control-blood type metrorrhagia and metrostaxis is prepared from liparis japonica, astragalus membranaceus, herba Begonia sinensis, white atractylodes rhizomes, Rhododendron mucronatum, Herba Ardisia mamillata Hance, RhizomaPanax japonicus, herb of bifurcate cinquefoil, wood of Fortune Windmillpalm and paper mulberry roots and can be used for clinically treating the qi-failing-to-control-blood type metrorrhagia and metrostaxis.

The invention discloses traditional Chinese medicine powder for treating diaper dermatitis of newborn. The traditional Chinese medicine thinks that the diaper rash is mainly caused by dampness, heat and toxin, and is applicable to clearing away heat and toxic materials, eliminating dampness and dispersing blood stasis, removing necrotic tissue and promoting granulation, and diminishing inflammation and relieving itching in treatment; therefore, the traditional Chinese medicines, namely gray vernonia, lemmaphyllum microphyllum, ardisia mamillata, broomjutre sida herb, puberulous glochidion herb root, adina pilulifera, radermachera sinica, abelmosk, cavalerie mosla herb and Japanese spiraea leaf are selected, are prepared into powder, and then are applied to the affected part. A clinical test shows that the total effective rate is 93.3%, and is significantly superior to 84.4% of a control group, and the traditional Chinese medicine powder is free of side effects.

The invention discloses medicine cake for treating bone fracture injury. The medicine cake is characterized by being prepared from the following herbal medicine in parts by weight: 4 parts of Liuyancai, 2 parts of Chinese wistaria, 2 parts of broussonetia kaempferi sieb, 2 parts of Yua thomsoni, 2 parts of Daphne limprichtii, 2 parts of sargentgloryvine stems, 2 parts of coniogramme japonica, 2 parts of Wo long grass, 2 parts of ardisia mamillata, 2 parts of osmanthus delavayi peel and leaves, 2 parts of camphor tree barks, 1 part of solanumindicuml stems, 1 part of lavicola, 1 part of Euphorbia erythrded hemsl roots and 1 part of pellionia radicans. According to a preparation method, the herbal medicine is proportionally crushed into medicine powder; then, rap oil and boiling water are added; the mixture is stirred into the medicine cake for external application on the wounded part, so that the medicine effect directly acts on the affected parts, so that the effects of clearing away heat and toxic materials, promoting qi circulation to relieve swelling, and promoting blood circulation to remove blood stasis are achieved, so that the effects of facilitating the poroma growth after the fast detumescence and treating the bone fracture injury are achieved. The medicine cake has the advantages that the preparation cost is low; the external application is convenient; the treatment effect is good; no side effect exists.

The invention discloses an ardisia mamillata grafting method, and belongs to the technical field of ornamental flower seedling growing. The method comprises the steps that robustly-growing annual or biennial ardisia crenata nutrient bag seedlings are adopted as stock, branches growing from the stock are removed, a 1.5 cm deep hole is cut in the pith, and a trunk is reserved; the annual half-lignification robust terminal buds of fine ardisia mamillata trees with the diameter being 0.2-0.5 cm are adopted as scions, the lower portions are cut into dual-face wedges and are inserted into gaps cut in the stock, the cambiums are tightly combined, then, a plastic strip is used for binding, the scions are covered with a transparent plastic bag, and the lower portions are fixed through aluminum sheets; grafting seedlings are placed on a seedbed, then, a transparent plastic cloth is used for covering the seedbed, after 20 days, the transparent plastic bag covering the scions is removed, the plastic cloth is properly disclosed, and conventional management is performed. By means of the grafting method, the ardisia mamillata morbidity is greatly lowered, maintenance workloads are small, the reproduction rate is high, the survival rate is high, plant types are attractive, the market requirement is greatly met, and the economic value is improved.

The invention discloses a grafting and seedling raising method for Ardisia mamillata Hance, and belongs to the technical field of seedling grafting. The grafting and seedling raising steps comprise rootstock selection, scion selection, scion cutting, rootstock cutting, scion cutting and binding, and post-grafting management, wherein in the post-grafting management step, the EC value of a culture medium adopted by a seedbed is 0.8-1.2 ms/cm. According to the method, grafting is carried out by utilizing twigs and tender shoots of the Ardisia mamillata Hance, excellent characters of a Ardisia mamillata Hance scion variety are kept; and meanwhile, a grafting culture medium is treated, the EC value is adjusted, meanwhile, the fertility of the medium is improved, the culture period is shortened,the disease probability of grafted seedlings is reduced, the grafting survival rate is increased, and the ornamental value of Ardisia mamillata Hance plants is improved.

The invention discloses a medicinal cake for treating wind heat type gonitis. The medicinal cake is characterized by being prepared from, by weight, 3 parts of symplocos chinensis, 1 part of ardisia mamillata, 1 part of Euphorbia hippocrepica Hemsl., 1 part of Bashan vein, 1 part of tylophora koi, 1 part of edgewortha meisn, 1 part of dolichopetalum kwangsiense, 1 part of pellionia radicans, 1 part of pilea notata, 1 part of anemone rivularis, 1 part of Elaeagnus gonyanthes, 1 part of prepared ranunculus chinensis, 1 part of furunculosis medicine and 1 part of Euphorbia erythrded hemsl. In order to improve the external application and treatment effect, a preparation method includes the steps that the 14 folk herbal medicines are prepared into medicine powder and stirred into the medicinal cake with rice water to be externally applied to affected parts, so that medicine effects reach lesions directly, the effects of clearing away heat and resolving dampness, drawing out toxin and activating stagnancy, reducing swelling and fever and expelling wind and promoting diuresis to eliminate dampness are achieved, and accordingly the effect of treating wind heat type gonitis and other fever and swelling type arthritis is achieved, wherein rice water can be replaced by saline water or a mirabilite aqueous solution. The medicinal cake has the advantages of being low in cost, convenient to use, reliable in treatment effect and free of toxic and side effects.

A traditional Chinese medicine composition for treating metrorrhagia and metrostaxis with kidney yin deficiency symptom is made from herb of Hypericum attenuatum, fresh Rehmannia root, common Macrocarpium fruit, Yerbadetajo herb, paper mulberry root, wood of fortune windmillpalm, herb of Ardisia mamillata, flower, root or stem-leaf of Rhododendron mucronatum, and rhizome of Panax japonicus. The traditional Chinese medicine composition is clinically applicable to treating metrorrhagia and metrostaxis with kidney yin deficiency symptom.

The invention relates to a traditional Chinese medicine for treating cervical spondylosis and rheumatoid arthritis. The traditional Chinese medicine is prepared from the following medicinal materials in parts by weight: 10 parts of piper kadsura, 3 parts of viburnum sargenti, 3 parts of small fruit fig aerial root, 1 part of scarlet pimpernel herb, 1 part of heterophyllous wing seedtree root, 2 parts of radix clematidis, 1 part of featherleaf rodgersflower, 15 parts of mustela eversmanni lesson, 0.5 part of gnetum parvifolium, 6 parts of herba eupatorii chinansis, 1 part of cryptotaenia japonica, 3 parts of siebold clubmoss herb, 20 parts of rough brake herb, 4 parts of indian glorybower herb, 10 parts of angelica gigas, 1 part of star-flower lysimachia, 2 parts of Chinese avens, 30 parts of funaria hygrometrica, 5 parts of schizophragma integrifolium, 3 parts of vaccinium dunalianum wight, 3 parts of ardisia mamillata, 1 part of oriental bittersweet root, 15 parts of rhizoma dioscoreae septemlobae, 6 parts of caulis erycibes and 17 parts of centella asiatica. Clinical trials show that the traditional Chinese medicine can be used for safely and effectively treating the cervical spondylosis and the rheumatoid arthritis.

The invention provides a traditional Chinese medicine composition for treating infantile malnutrition and a preparation method thereof, and relates to the technical field of traditional Chinese medicine preparation. The traditional Chinese medicine composition is mainly prepared from rungia pectinata, cynanchum wilfordii, berchemia racemosa, cucubalus baccifer, mimosa pudica, drymaria diandra, roots of aeschynomene indica, ardisia mamillata, herba taraxaci, primula sinopurpurea, common bluebeard herb, thalictrum esquirolii Levl. et Van., dendrobenthamia capitata (Wall.)Hutch., boerhaavia diffusa L. and pericarpium citri reticulatae. With the scientific compatibility and synergism of the raw materials, the traditional Chinese medicine composition has the effects of tonifying the spleen and relieving malnutrition, and expelling parasites and removing stagnation, has a good treatment effect on infantile malnutrition, and is high in pertinence, free of toxic or side effects and worthy of application and popularization.

The invention discloses rochas winter ointment for treating frostbite and a preparation method thereof. The rochas winter ointment is prepared by mistletoe, ardisia mamillata, basil, oenothera odorata, rubus parvifolius oil, amaranthus caudatus and ampelopsis aconitifolia by weight. The rochas winter ointment has functions of clearing away heat and toxic materials, promoting granulation, relieving pain, removing carbuncle and stasis, is used for treating the frostbite, and is good in curative effect, rapid in effect production and high in cure rate.

The invention discloses a method for preparing ardisia mamillata extract. The method comprises the following steps: (1) washing fresh solid-liquid ratio, cutting into pieces, homogenizing with water to prepare slurry; (2) adding a petroleum ether extraction volatile component into the slurry, separating a petroleum ether extraction component, and concentrating the rest slurry to be less than the weight of previous ardisia mamillata to obtain extract; (3) adding an alcohol solution having a volume percentage of 60-70 percent into the extract in a solid-liquid ratio of 25-30mL/g, and insulatingthe pressure at 150-200 MPa for 8-10 minutes; and (4) concentrating the extract, passing through a microporous adsorption resin column, eluting with water, 30% alcohol and 80% alcohol sequentially, collecting the 80% alcohol eluted liquid, and concentrating and drying at reduced pressure to obtain the ardisia mamillata extract. The invention further discloses a method for detecting the ardisia mamillata extract. The method has simple steps and convenience in operation, and has high content of extracted saponin ingredient and accurate and reliable result.

The invention belongs to the technical field of ardisia mamillata plant tissue culture, and particularly discloses a stem disinfection and proliferation induction method for ardisia mamillata. The method comprises the steps of branch acquisition, stem pretreatment and disinfection, primary culture, multiplication culture and culture subculture multiplication. By adopting a disinfection reagent and the disinfection time, the stems with a relatively low pollution rate are obtained, and the pollution rate is only 36.3%; the stems with buds are subjected to primary germination induction for 20-60 days, so that a higher germination rate is obtained, and the germination rate is 90.9%; proliferation culture is carried out on the germinated stems for 30-60 days, and the proliferation coefficient is 8.33; and the culture is subjected to subculture for 45-55 days, the average proliferation coefficient is 10.83, and the highest proliferation coefficient is 22.0. According to the method, the proper disinfection reagent and disinfection time, stem primary culture and proliferation culture media and a culture subculture culture medium are screened out, a large number of red bud clusters are obtained, a technical guarantee is provided for reserving excellent characteristics of a red single plant of the ardisia mamillata, improving the ornamental value of the ardisia mamillata and subsequent seedling breeding, and a foundation is laid for industrial development.

The invention discloses fractured joint stiffness fuming and washing gristle soup which is prepared from 13 herbal medicines in proportion. Chinese wistaria, Dalbergia dyeriana, grape blood vines, curly ardisia japonica and Dregea sinensis have the functions of relaxing muscles and tendons, smoothing collaterals, diminishing swelling, dispersing blood stasis, eliminating dampness and removing paralysis. Ardisia mamillata, paramichelia, wild lychees and elaeagnus henryi have the functions of promoting tissue regeneration, expelling decay and promoting reunion of fractured bones. Celtis sinensis and Zanthoxylum ailanthoides have the functions of preventing skin itch, expelling wind and cooling blood. Salvia liguliloba and hance rosewood have the functions of promoting qi circulation to relieve pain, widening tendons and softening connective tissues. A patient suffering from fractured joint stiffness is fumed, washed and soaked, circulation is improved, metabolism is promoted, and the connective tissues are softened, so that meridians are dredged, adhesion is relaxed, and activity functions are gradually improved. The gristle soup is pure natural plant liquid for external use and has the advantages of low preparation cost, convenience in use, good treatment effect and no toxic and side effects.

The invention discloses a traditional Chinese medicine preparation for preventing OCS (osteofascial compartment syndrome) after splint fixation. OCS is a disease which has a feature of acute and severe ischemia of muscles and nerves due to microcirculatory mal-perfusion of tissue under increased osteofascial compartmental pressure for various reasons. Traditional Chinese medicines comprising begonia yunnanensis levl, euonymus oxyphyllus, herbs of Japanese ophiorrhiza, ardisia mamillata hance, Chinese siphonostegia herbs, roots of jute, photinia parvifolia, winged yam rhizomes, amphicome, herbs of chien briggsia,, all-grass of capitatestigma willowweed and indigofera mengtzeana are selected and decocted with water into decoction. A clinical test shows that the traditional Chinese medicine preparation is remarkable in curative effect and worth popularizing and using clinically.

The invention discloses a traditional Chinese medicine composition for controlling chicken encephalomyelitis and a preparing method. The traditional Chinese medicine composition comprises cortex acanthopanacis, blumea, liquorice, herbs of hokker winghead, rapeseeds, hemistepta lyrata, pararuellia delavayana, fructus forsythiae, dandelions, roots of griffith streptocaulon, lonicera japonica, isatis roots, sophora vicifolia, nauclea officinalis, four-leaf clovers, roots or branch leaves of lindera angustifolia cheng, serissa foetida comm, ardisia mamillata hance, laggera alata, rose mallow roots, malt, rhizomes of David woodbetony, phoenix tree seeds, iris confusa sealy, acanthopanax roots, ficus esquiroliana and roots of featherycleft horsegentian. The traditional Chinese medicine composition has the advantages of being used for controlling chicken encephalomyelitis, capable of improving the organism immunity of stricken chickens, short in treatment course, good in curative effect, not prone to relapse after curing, free of medicine resistance, free of medicine resistance after long-time application, free of medicine residues and low in cost price.