Mature embryo callus induction and differentiation and adventitious bud proliferation method for ardisia mamillata

A mature embryo and tiger tongue technology, which is applied in the field of tiger tongue plant tissue culture, can solve the problems of low proliferation coefficient and difficult disinfection, and achieve the effect of easy disinfection, convenient and quick operation, and easy handling

Active Publication Date: 2021-07-13
贵州省林业科学研究院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The object of the present invention is to provide a method for the callus induction and differentiation of mature embryos of Hutonghong and the proliferation of adventitious buds, so as to solve the problems of difficult disinfection and low proliferation coefficient in the tissue culture of Hutonghong.

Method used

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  • Mature embryo callus induction and differentiation and adventitious bud proliferation method for ardisia mamillata
  • Mature embryo callus induction and differentiation and adventitious bud proliferation method for ardisia mamillata
  • Mature embryo callus induction and differentiation and adventitious bud proliferation method for ardisia mamillata

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Embodiment 1

[0028] A method for callus induction and differentiation of mature embryos of Hutonghong and adventitious bud proliferation, comprising the following steps:

[0029] (1). Seed treatment: take the mature fruit on the plant without diseases and insect pests and grow vigorously, first wash and remove the outer testa with soapy water, then rinse with clear water to remove the submerged seeds, and finally use the flowing water to remove the seeds Rinse with clean water for 30 minutes and transfer to ultra-clean workbench;

[0030] (2). Sterile seeds: In the ultra-clean workbench, soak the seeds in step (1) with 70% to 75% alcohol for 30 to 60s, and 1% to 2% NaClO for 5 to 8 minutes, with an interval of 10 to 30s Shake once, then rinse with sterile water for 3 to 5 times to obtain sterile seeds;

[0031] (3). Callus induction and differentiation induction: After the sterile seeds obtained in step (2) are blotted dry with sterilized filter paper, the seeds are cut open with steriliz...

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Abstract

The invention belongs to the technical field of ardisia mamillata plant tissue culture, and particularly discloses a mature embryo callus induction and differentiation and adventitious bud proliferation method for ardisia mamillata. The mature embryo callus induction and differentiation and adventitious bud proliferation method for the ardisia mamillata comprises the steps of mature fruit acquisition, seed pretreatment, seed disinfection, embryo acquisition, callus induction, differentiation and adventitious bud proliferation culture. On an ultra-clean workbench, mature seeds are disinfected, embryos are taken out and inoculated to a culture medium, and the embryo pollution rate is 0; after culture is performed for 60 to 80 days, the induction rate of calluses is 90 percent, and the differentiation rate of the calluses is 48 percent; differentiated adventitious buds are inoculated into a proliferation culture medium to be continuously cultured for 45-55 days, and the proliferation rate of the adventitious buds can reach 1600% or above. According to the test, an efficient propagation method is provided for cultivation of the ardisia mamillata, the mature embryos serve as explants, the pollution rate is low, the induction rate is high, the cultivation period is short, the number of the adventitious buds is large, and a foundation is laid for propagation of the ardisia mamillata, development of new varieties and protection of good varieties.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture of tiger tongue red, and specifically discloses a method for callus induction and differentiation of mature embryos of tiger tongue red and proliferation of adventitious buds. Background technique [0002] Ardisia mamillata Hance (Ardisia mamillata Hance) is a dwarf shrub of the Ardisia mamillata family, native to China, with a flowering period from June to August. The leaves are red or green, the whole plant is covered with white or red hairs, and the fruit is bright red when ripe. Anniversary fruiting, fruit and leaves show a festive and peaceful ornamental effect, it is the first choice for indoor foliage plants. In addition, the whole herb of tiger tongue red is used as medicine, which has the functions of clearing heat and dampness, promoting blood circulation and stopping bleeding, removing rot and muscle, and has extremely high medicinal value. [0003] At present, the propaga...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/00
Inventor 邓伦秀吴高殷刘晓慧陈锐龙海燕徐超然
Owner 贵州省林业科学研究院
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