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Ex-vitro rooting method for ardisia mamillata tissue culture seedlings

A tiger tongue group and external rooting technology, which is applied in the direction of botany equipment and methods, cultivation, medium, etc., can solve the problems of long rooting production cycle, high production cost, low transplanting survival rate, etc., and achieve high rooting rate , easy to operate, and low infection rate

Pending Publication Date: 2021-12-03
贵州省林业科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a method for rooting out of the bottle of tiger tongue tissue cultured seedlings, to solve the problems of long production cycle, low transplanting survival rate and high production cost of tiger tongue tissue cultured seedlings rooting in the bottle

Method used

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  • Ex-vitro rooting method for ardisia mamillata tissue culture seedlings
  • Ex-vitro rooting method for ardisia mamillata tissue culture seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1, substrate and rooting agent screening

[0050] A method for rooting outside the bottle of Hutonghong tissue culture seedlings, comprising the following steps:

[0051] (1) Seedling box: The seedling box is composed of a cover, a hole tray and a water tray. The number of holes is 3×4 with 12 holes ( figure 1 );

[0052] (2) Substrate and treatment: On September 15, 2020, the rooting substrate was new vermiculite, perlite, and peat (Table 1) in the form of 1-6 mm particles. First, the substrate was sterilized in an autoclave, and then Put it into the hole tray and compact it, and finally put the hole tray into the water receiving tray that has been added with 600-800ml deionized water, so that the matrix can absorb water and saturate automatically;

[0053] (3) cuttings making: select the bottle seedlings with good growth of clustered buds, stems, leaves and hairs in red, take out the clustered buds and cut them into stems with terminal buds, and cut off ...

Embodiment 2

[0064] Embodiment 2, improve nursery box and continue verification test in cultivation room

[0065] A method for rooting outside the bottle of Hutonghong tissue culture seedlings, comprising the following steps:

[0066] (1) Seedling box: The seedling box is composed of a cover, a hole tray and a water tray. 24 points with 6×4 points ( Figure 4 );

[0067] (2) Substrate and treatment: The rooting substrate is 1-3mm granular vermiculite, put the substrate into a hole tray, compact it, put the hole tray into a water tray with about 1300ml of tap water added, and let the substrate absorb water and saturate automatically ( Figure 5 );

[0068] (3) Cutting production: select the bottle seedlings with good growth of clustered buds, red stems, leaves and hairs ( Image 6 ), take out the bottle seedlings, cut the clustered buds with a thickness of ≥1.5mm into 7-8cm long stems with terminal buds, and cut off the leaves at the base of the stems at 1-3cm as cuttings ( Figure 7 ...

Embodiment 3

[0074] Embodiment 3, carry out verification test in greenhouse seedbed with forest plug

[0075] A method for rooting outside the bottle of Hutonghong tissue culture seedlings, comprising the following steps:

[0076] (1) Seedling tray: The seedling tray is a tree tray, the length, width, and depth are 54 cm × 28 cm × 9 cm, the upper hole is 5 cm × 5 cm, the lower hole is 1.5 cm × 1.5 cm, the number of holes 50 holes of 5×10 ( Figure 19 );

[0077] (2) Substrate and plate: the rooting substrate is the same as in Example 2, except that the amount of tap water is not controlled, that is: 1~3mm granular vermiculite, the matrix is ​​packed into the plug tray, compacted, and the plug tray is put into the In the water container of tap water, let the matrix absorb water and soak it automatically, and finally place the hole tray on the seedbed of the greenhouse ( Figure 19 );

[0078] (3) cuttings are made: cuttings are made the same as embodiment 2, just do not control the leng...

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Abstract

The invention belongs to the technical field of ardisia mamillata tissue culture, and particularly discloses an ex-vitro rooting method for ardisia mamillata tissue culture seedlings, which comprises the steps of seedling raising tray preparation, water preparation, matrix preparation, rooting agent preparation, bottle seedling preparation, cutting treatment, maintenance management, and root promoting and seedling strengthening. According to the adopted matrix and culture conditions, the pollution rate of the cuttings is almost 0, the cuttings start to root after being cultured for 15-20 days, the rooting rate reaches 100% after being cultured for 25-30 days, the average root length is 3.4 cm, the number of root points is 4.7, the root width is 4-5 cm, the average root length is 6.5 cm, the number of root points is 5.5, and the root width is 6-8 cm after being subjected to cuttage culture on a greenhouse seedbed for about 30 days, and seedlings with good root clusters are formed. The efficient rooting method is provided for ex-vitro rooting of the ardisia mamillata tissue culture seedlings, compared with in-bottle rooting, the root system quality is improved, the rooting time is shortened, the rooting procedure is optimized, the production cost is reduced, the method is simple, operability is high, and technical support can be provided for development of the whole industry chain of ardisia mamillata.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture of tiger tongue red, and specifically discloses a method for rooting out of a bottle of tiger tongue red tissue cultured seedlings. Background technique [0002] Tiger Tongue Red (Ardisia mamillata Hance), also known as red felt and hairy parasol, is a small perennial evergreen shrub of the genus Ardisia mamillata Hance. , was discovered and introduced and cultivated in the deep mountains and old forests in Dayu County, Jiangxi Province. The whole plant is purple-red, with obovate leaves, fine serrations on the leaf margins, sparse glandular spots on both sides of the front and back, densely covered with thick purple-red thick long hairs, resembling a tiger's tongue. The flowering period of tiger tongue red is from June to August. The fruit is bright red after ripening, and bears fruit every year. [0003] At the Kunming World Horticultural Exposition in 1999, the tiger tongue red e...

Claims

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Application Information

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IPC IPC(8): A01G9/029A01G17/00A01G24/15
CPCA01G9/0295A01G17/005A01G24/15
Inventor 邓伦秀刘晓慧吴高殷李鹤陈菊艳陈志萍
Owner 贵州省林业科学研究院
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