Stem disinfection and proliferation induction method for ardisia mamillata
A technology of Rhizoma rhizoma and stem segment, which is applied in the field of stem segment sterilization and proliferation induction of Rhizoma rhodozyma, and can solve the problems of high contamination rate of stem segment and low proliferation coefficient, etc.
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[0035] A method for disinfecting and proliferating the stem segment of Hutonghong, comprising the following steps:
[0036] (1) Acquisition of branches: select a red plant that grows robustly and has no damage by diseases and insect pests, and cuts the long main branch of 5~10cm ( figure 1 );
[0037] (2) Stem section pretreatment: the main branch of step (1) is cut off the leaves, put into a beaker of 400-500ml water, first drop 4-5 drops of detergent and put it on a magnetic stirrer to stir for 10-15min, then Wash it with a toothbrush, transfer it to running water under tap water for 20-30 minutes, and finally cut it into 3-5cm long stems ( figure 2 );
[0038] (3) Disinfection of stems: on the ultra-clean workbench, treat the stems in step (2) with 70% to 75% ethanol for 1 to 2min, 0.1% to 0.2% HgCl 2 Treat for 10 to 15 minutes, 1% to 2% NaClO for 2 to 5 minutes, shake once every 10 to 30 seconds, and finally wash with sterile water for 3 to 5 times, and put it into a s...
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