Stem disinfection and proliferation induction method for ardisia mamillata

A technology of Rhizoma rhizoma and stem segment, which is applied in the field of stem segment sterilization and proliferation induction of Rhizoma rhodozyma, and can solve the problems of high contamination rate of stem segment and low proliferation coefficient, etc.

Active Publication Date: 2021-08-13
贵州省林业科学研究院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The object of the present invention is to provide a method for disinfection and multiplication induction of Rhizoma chinensis st

Method used

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  • Stem disinfection and proliferation induction method for ardisia mamillata
  • Stem disinfection and proliferation induction method for ardisia mamillata
  • Stem disinfection and proliferation induction method for ardisia mamillata

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Embodiment 1

[0035] A method for disinfecting and proliferating the stem segment of Hutonghong, comprising the following steps:

[0036] (1) Acquisition of branches: select a red plant that grows robustly and has no damage by diseases and insect pests, and cuts the long main branch of 5~10cm ( figure 1 );

[0037] (2) Stem section pretreatment: the main branch of step (1) is cut off the leaves, put into a beaker of 400-500ml water, first drop 4-5 drops of detergent and put it on a magnetic stirrer to stir for 10-15min, then Wash it with a toothbrush, transfer it to running water under tap water for 20-30 minutes, and finally cut it into 3-5cm long stems ( figure 2 );

[0038] (3) Disinfection of stems: on the ultra-clean workbench, treat the stems in step (2) with 70% to 75% ethanol for 1 to 2min, 0.1% to 0.2% HgCl 2 Treat for 10 to 15 minutes, 1% to 2% NaClO for 2 to 5 minutes, shake once every 10 to 30 seconds, and finally wash with sterile water for 3 to 5 times, and put it into a s...

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Abstract

The invention belongs to the technical field of ardisia mamillata plant tissue culture, and particularly discloses a stem disinfection and proliferation induction method for ardisia mamillata. The method comprises the steps of branch acquisition, stem pretreatment and disinfection, primary culture, multiplication culture and culture subculture multiplication. By adopting a disinfection reagent and the disinfection time, the stems with a relatively low pollution rate are obtained, and the pollution rate is only 36.3%; the stems with buds are subjected to primary germination induction for 20-60 days, so that a higher germination rate is obtained, and the germination rate is 90.9%; proliferation culture is carried out on the germinated stems for 30-60 days, and the proliferation coefficient is 8.33; and the culture is subjected to subculture for 45-55 days, the average proliferation coefficient is 10.83, and the highest proliferation coefficient is 22.0. According to the method, the proper disinfection reagent and disinfection time, stem primary culture and proliferation culture media and a culture subculture culture medium are screened out, a large number of red bud clusters are obtained, a technical guarantee is provided for reserving excellent characteristics of a red single plant of the ardisia mamillata, improving the ornamental value of the ardisia mamillata and subsequent seedling breeding, and a foundation is laid for industrial development.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture of tiger tongue red, and specifically discloses a method for disinfection and proliferation induction of tiger tongue red stem segments. Background technique [0002] Tiger Tongue Red (Ardisia mamillata Hance), a perennial evergreen shrub of the family Ardisia mamillata Hance, its leaves are green or dark purple on both sides, and are all covered with downy hairs, with beautiful luster; the flowers are in umbels; the fruits are bright red, The fruit period is long, and it is an ornamental plant integrating foliage, flower and fruit viewing; its roots, stems, and leaves are all Chinese medicinal materials, with high ornamental and medicinal values. Tiger Tongue Red is a rare flower in the world, with few wild resources. It mainly grows in the mountains, bamboo forests and shrubs. It is scattered and has poor self-renewal ability. It is an endangered plant in the world. Due to the incr...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008Y02P60/40
Inventor 吴高殷邓伦秀刘晓慧陈锐龙海燕徐超然
Owner 贵州省林业科学研究院
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