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Explant disinfection method for culture of hair bearing plant tissue

A technology of plant tissue culture and disinfection method, which is applied in the field of explant disinfection during plant tissue culture with hairy surfaces, and can solve the problems of high pollution rate and incomplete disinfection

Inactive Publication Date: 2015-09-23
THE CITY VOCATIONAL COLLEGE OF JIANGSU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is that the conventional disinfection method for explants in plant tissue culture is not suitable for explants covered with hairs all over the plant, which often leads to incomplete disinfection and high pollution rate.

Method used

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  • Explant disinfection method for culture of hair bearing plant tissue
  • Explant disinfection method for culture of hair bearing plant tissue
  • Explant disinfection method for culture of hair bearing plant tissue

Examples

Experimental program
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Effect test

Embodiment 1

[0025] 1. Pretreatment of female plants

[0026] Move it into the greenhouse one month before the shoot of the female parent plant, and carry out certain fertilizer and water management and pest control. Spray carbendazim with 800 times of liquid of 50% wettable powder on the plants two days before taking materials. In this way, the pretreatment of experimental materials can not only reduce the number of active microorganisms hidden on the surface of explants and hairs, but also reduce the probability of microbial infection in the process of plant tissue culture, and can also maintain the consistency of materials during the experiment, thereby improving Experimental repeatability.

[0027] 2. The period of collecting materials

[0028] In mid-March, mid-May, mid-August and mid-November, the terminal buds and leaves of the current year were cut for experiments. The experimental results (see Table 1) show that August is the period with the highest pollution rate of tiger tong...

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Abstract

The invention relates to an explant disinfection method for culture of hair bearing plant tissue, and belongs to the technical field of plant tissue culture in the technical field of biotechnology. The explant disinfection method for culture of hair bearing plant tissue of the invention comprises: pre-culturing a mother plant; pre-treating the mother plant by using carbendazim; disinfecting the explant by using alcohol; then performing mercuric chloride based disinfection twice, wherein disinfection is performed by using mercuric chloride for 4 minutes, rinsing is performed five times by using sterile water, then disinfection is performed by using mercuric chloride for 4 minutes, and rinsing is performed five times by using sterile water; and then performing seeding in a start culture medium that contains yipeilong. The method can eliminate contamination bacteria on the surface of the ardisia mamillata explant, and keep the explant robust.

Description

technical field [0001] The invention relates to a method for disinfecting explants during plant tissue culture with hairy surfaces, and belongs to the technical field of plant tissue culture in the field of biotechnology. Background technique [0002] Tiger Tongue Red belongs to Ardisia mamillata Hance (Myrsinaceae) and is a perennial evergreen shrub. Tiger tongue red is covered with hairs all over the body and contains a lot of dirt, which makes it very difficult to disinfect the surface of explants during tissue culture, often resulting in incomplete disinfection and high pollution rate or excessive disinfection leading to loss of explant vitality , it is more difficult to obtain sterile materials. Therefore, the acquisition of effective sterile materials is the key to the tissue culture of Hutonghong. [0003] The conventional disinfection method for explants in plant tissue culture is to rinse with running water first, then disinfect with alcohol for 30 seconds to 1 mi...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 曾云英韩承辉符嫦娥叶海跃万强马存琛
Owner THE CITY VOCATIONAL COLLEGE OF JIANGSU
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