Preparation method of Ardisia mamillata B and uses thereof

A technology for the use of tiger tongue red pigment, which is applied in the field of medicine, can solve the problems of narrow application range and high cost of extraction and separation, and achieve the effects of stable process, significant pharmacological activity and low cost

Active Publication Date: 2011-05-11
四川恩威制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the disadvantages of high extraction and separation cost and narrow application range of abatin B in the prior art, and provide people with a low-cost preparation method of ababatin B, and a kind of preparation method of ababatin B As a drug for the prevention and treatment of retrovirus infection and pathogenic microorganisms including tumors, AIDS, leukemia, influenza, herpes, hepatitis, and HIV, and as an anti-inflammatory and analgesic drug

Method used

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  • Preparation method of Ardisia mamillata B and uses thereof
  • Preparation method of Ardisia mamillata B and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The preparation of tiger tongue red pigment B: get tiger tongue red crude drug (dry product) 1kg and be pulverized into 20 order coarse powders, add 80% (weight) ethanol aqueous solution of 10 times of medical material weight, heat and reflux extraction 3 times, each 2 hours, Filtrate, combine the extracts, recover the solvent under reduced pressure and concentrate to 1g crude drug / mL, absorb with D101 macroporous resin, wash with water, then elute with 80% (weight) ethanol aqueous solution, and the ethanol eluate is concentrated until the extractum is obtained effectively Part 39g, the effective part is mixed with equal weight silica gel sample, 30 times (weight) extract amount silica gel packing column, ethyl acetate: methanol=8: 3 (weight) elution, collect the distillate after twice column volume, concentrate Crystallization yielded 21 g of tiger lipin B light white powder.

Embodiment 2

[0023] Preparation of tiger tongue red pigment B: take 1 kg of tiger tongue red medicinal material (fresh product) and pulverize it into 60 meshes, add 75% (weight) ethanol aqueous solution 10 times the weight of the medicinal material, ultrasonically extract 3 times, each time for 20 min, filter, and combine The extract is recovered under reduced pressure and the solvent is concentrated to 1g of crude drug / mL, adsorbed by D141 macroporous resin, washed with water, eluted with 75% (weight) ethanol aqueous solution, concentrated to extractum to obtain 23g of effective part, and the effective part is obtained by equal weight Mix the sample with silica gel, pack 30 times (weight) of the extract into a silica gel column, elute with ethyl acetate:methanol=8:3 (weight), collect the fraction after twice the column volume, concentrate to the extract, and analyze after ultrasonic dissolution in ethanol crystallized, filtered, and the precipitate was recrystallized with ethanol to obtain...

Embodiment 3

[0025] The preparation of tiger tongue red pigment B: get tiger tongue red decoction piece 1kg to be pulverized into 60 order, with 75% (weight) ethanol aqueous solution percolation extraction of 20 times of medical material weight, extract is filtered, decompression recovery solvent is concentrated to 1g crude drug / mL, adsorbed by AB-8 macroporous resin, washed with water, eluted with 75% (weight) ethanol aqueous solution, concentrated to the extract to obtain 30g of effective part, the effective part was mixed with equal weight silica gel, soaked 30 times (weight) Paste volume Silica gel column, ethyl acetate: methanol = 8: 3 (weight) elution, collect the distillate after twice the column volume, concentrate to extract, acetone: methanol = 1: 1 (weight) ultrasonic dissolution and crystallization , filtered, and the precipitate was recrystallized with acetone:methanol=1:1 (weight) to obtain 14g of abatin B light white powder.

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Abstract

The invention pertains to the pharmaceutical technical field, which relates to a preparation method and the usage of ardisia mamillata B, the invention takes the whole trees or branches and leaves or roots and rhizomes of the ardisia mamillata as the medicines, the water, methanol water solution with an arbitrary proportion, ethanol water solution or organic solvent are used for extraction, the extract liquid is filtered and concentrated; the concentrate is prepared by recrystallization and refining after the independent or combined separation by macroporous resin, column chromatography(alumina or silica gel). The invention has the advantages that: the invention provides the preparation method of ardisia mamillata B which can be used for anti-tumor, anti-inflammation, analgesia, anti-virus (such as, influenza viruses, herpes viruses, hepatitis viruses, anti-retroviral viruses including HIV and so on), the cost is low, the process is stable, the yield is more than 1 percent of the dry herbs, the invention can obtain the ardisia mamillata B with high purity which has been removed a large amount of impurities, and the pharmacological activity is significant.

Description

Technical field: [0001] The invention belongs to the technical field of medicine, and in particular relates to a preparation method and application of tiger tongue B. Background technique: [0002] Tiger tongue red (Ardisia mamillata Hance) is a plant of the genus Ardisia mamillata Hance, and its branches, leaves and rhizomes are all used as medicine. It grows in shady forests at an altitude of 200-400 meters, likes shade, and grows in groups. There are more than 300 species of this genus in the world, and 68 species and 12 varieties in my country. It is widely distributed in the vast area south of the Yangtze River and has rich reserves. Especially concentrated in Yunnan and Hainan. Tiger tongue red is believed to have the effect of clearing away heat and dampness, promoting blood circulation and removing blood stasis in traditional Chinese medicine. According to the records in the "Dictionary of Chinese Medicine", the plant can be used to treat diseases such as dysente...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J71/00A61K31/7048A61P35/00A61P31/18A61P1/16A61P31/12A61K36/185
Inventor 薛永新薛永江薛刚薛洪
Owner 四川恩威制药有限公司
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