Preparation method of Ardisia mamillata B and uses thereof
A technology for the use of tiger tongue red pigment, which is applied in the field of medicine, can solve the problems of narrow application range and high cost of extraction and separation, and achieve the effects of stable process, significant pharmacological activity and low cost
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Embodiment 1
[0021] The preparation of tiger tongue red pigment B: get tiger tongue red crude drug (dry product) 1kg and be pulverized into 20 order coarse powders, add 80% (weight) ethanol aqueous solution of 10 times of medical material weight, heat and reflux extraction 3 times, each 2 hours, Filtrate, combine the extracts, recover the solvent under reduced pressure and concentrate to 1g crude drug / mL, absorb with D101 macroporous resin, wash with water, then elute with 80% (weight) ethanol aqueous solution, and the ethanol eluate is concentrated until the extractum is obtained effectively Part 39g, the effective part is mixed with equal weight silica gel sample, 30 times (weight) extract amount silica gel packing column, ethyl acetate: methanol=8: 3 (weight) elution, collect the distillate after twice column volume, concentrate Crystallization yielded 21 g of tiger lipin B light white powder.
Embodiment 2
[0023] Preparation of tiger tongue red pigment B: take 1 kg of tiger tongue red medicinal material (fresh product) and pulverize it into 60 meshes, add 75% (weight) ethanol aqueous solution 10 times the weight of the medicinal material, ultrasonically extract 3 times, each time for 20 min, filter, and combine The extract is recovered under reduced pressure and the solvent is concentrated to 1g of crude drug / mL, adsorbed by D141 macroporous resin, washed with water, eluted with 75% (weight) ethanol aqueous solution, concentrated to extractum to obtain 23g of effective part, and the effective part is obtained by equal weight Mix the sample with silica gel, pack 30 times (weight) of the extract into a silica gel column, elute with ethyl acetate:methanol=8:3 (weight), collect the fraction after twice the column volume, concentrate to the extract, and analyze after ultrasonic dissolution in ethanol crystallized, filtered, and the precipitate was recrystallized with ethanol to obtain...
Embodiment 3
[0025] The preparation of tiger tongue red pigment B: get tiger tongue red decoction piece 1kg to be pulverized into 60 order, with 75% (weight) ethanol aqueous solution percolation extraction of 20 times of medical material weight, extract is filtered, decompression recovery solvent is concentrated to 1g crude drug / mL, adsorbed by AB-8 macroporous resin, washed with water, eluted with 75% (weight) ethanol aqueous solution, concentrated to the extract to obtain 30g of effective part, the effective part was mixed with equal weight silica gel, soaked 30 times (weight) Paste volume Silica gel column, ethyl acetate: methanol = 8: 3 (weight) elution, collect the distillate after twice the column volume, concentrate to extract, acetone: methanol = 1: 1 (weight) ultrasonic dissolution and crystallization , filtered, and the precipitate was recrystallized with acetone:methanol=1:1 (weight) to obtain 14g of abatin B light white powder.
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