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Preparation method and system for energy imbalance type obese gene individual intervention drink

A technology of obesity genes and beverages, applied in biochemical equipment and methods, microbe measurement/testing, food science, etc., can solve problems affecting energy metabolism, diversity changes, etc.

Inactive Publication Date: 2015-12-09
上海中优精准医疗科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mutations in the ADRB2 gene can lead to changes in receptor expression and functional diversity, thereby affecting energy metabolism

Method used

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  • Preparation method and system for energy imbalance type obese gene individual intervention drink
  • Preparation method and system for energy imbalance type obese gene individual intervention drink
  • Preparation method and system for energy imbalance type obese gene individual intervention drink

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Example 1 Establishment of Joint Genotype Risk Level 1

[0076] The polymorphic site detected in the present embodiment is: rs1800592 ( UCP1 ), rs660339 ( UCP2 ) and rs1042713 ( ADRB2 ), mark them as a, b, and c respectively, and their subtype risks are shown in Table 8 below:

[0077] Table 8

[0078] genetic polymorphism site genotype Risk (OR) a 1(AA) 1

[0079] The risk grades calculated according to the single gene risk listed in Table 8 are shown in Table 4 above.

Embodiment 2

[0080] Example 2 Establishment of Joint Genotype Risk Level II

[0081] The polymorphic site detected in the present embodiment is: rs1800592 ( UCP1 ), rs660339 ( UCP2 ), rs1042713 ( ADRB2 ) and rs1042714 ( ADRB2 ), mark them as a, b, c, and d respectively, and their subtype risks are shown in Table 9 below:

[0082] Table 9

[0083] genetic polymorphism site genotype Risk (OR) a 1(AA) 1

2(AG) 2.63

3(GG) 2.63 b 1(CC) 1

2(CT) 1

3(TT) 2.22 c 1(AA) 1

2(AG) 1

3(GG) 4.12 d 1(CC) 1

2 (CG) 2.66

3(GG) 2.66

[0084] The risk level calculated based on the single gene risk listed in the above table is shown in Table 10 below:

[0085] Table 10

[0086]

Embodiment 3

[0087] Embodiment 3 Individual gene detection one

[0088] The 3 polymorphic sites in Example 1 were used for individual gene detection.

[0089] Individual oral epithelial cell samples were collected, and genomic DNA was extracted by silica gel adsorption method. After electrophoresis detection and verification, fluorescent quantitative PCR reaction was performed.

[0090] Put individual samples into 3 reaction wells respectively, and detect 3 sites at the same time, namely rs1800592( UCP1 ), rs660339 ( UCP2 ), rs1042713 ( ADRB2 ), and additional NTC blank control wells without DNA templates were added according to the experimental needs;

[0091] Add reagents to each reaction well as a fluorescent quantitative PCR reaction system with a total volume of 10 μL, that is, 2 μL of DNA template with a concentration of 20 ng / μL, 10× fluorescent quantitative PCR reaction buffer ABITaqman ? MGB 1 μL, 25mM dNTP four deoxynucleotide substrates for DNA synthesis 0.1 μL, 25mM MgCl ...

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Abstract

The invention discloses a preparation method and system for an energy imbalance type obese gene individual intervention drink. The provided preparation method comprises four steps as follows: establishment of combined genotype risk grades; individual gene detection; determination of a drink formula according to the risk grade of a detection result; preparation of the drink. A targeted weight-reducing drink can be prepared according to the combined genotype of an obese individual, and the integrated process from sample detection to drink preparation is realized with the preparation system of the individual intervene drink; the prepared individual intervention drink has a good taste and is popular with people.

Description

technical field [0001] The invention relates to an individualized intervention drink for obesity genes, in particular to a preparation method and system for an individualized intervention drink for energy imbalance type obesity genes. Background technique [0002] Obesity is a common disease that seriously endangers people's health. In 1999, WHO defined central obesity as one of the components of metabolic syndrome. In my country and the world, the incidence of obesity is increasing year by year. The World Health Organization Expert Committee proposes to use Body-Mass Index (BMI) as the standard for overweight or obesity, that is, a BMI between 25 and 28 is considered overweight, 28 to 32 is considered obese, and a BMI greater than 32 is considered very obese. Obesity is a chronic disease closely related to genetic factors and lifestyle. It can cause elevated blood lipids, which can easily lead to fatty liver, arterial vascular disease, heart disease, and diabetes. Obese ch...

Claims

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Application Information

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IPC IPC(8): C12Q1/68A23L2/02
CPCC12Q1/6883A23L2/02C12Q1/6827C12Q2600/156
Inventor 毛丹丹王校张奕傅咏南邹创卞雪莲何骏奇龚丽
Owner 上海中优精准医疗科技股份有限公司
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