A method for measuring antibody titer by using antigen one-way diffusion method

A technology of antibody titer and one-way diffusion, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of cumbersome measurement process and deviation of measurement results, and achieve fast and accurate results, simple operation and accurate measurement.

Active Publication Date: 2017-03-22
山西瑞亚力生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the technical problems that existing antibody titer assay methods easily lead to deviations in the measurement results and the measurement process is cumbersome, and provide a method for assaying antibody titer using the antigen one-way diffusion method

Method used

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  • A method for measuring antibody titer by using antigen one-way diffusion method
  • A method for measuring antibody titer by using antigen one-way diffusion method
  • A method for measuring antibody titer by using antigen one-way diffusion method

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Experimental program
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Embodiment 1

[0017] A method for determining antibody titer using the antigen unidirectional diffusion method in this embodiment comprises the following steps:

[0018] 1) Seal one end of a graduated glass tube with adhesive tape, the inner diameter of the glass tube is 4mm, and the outer diameter is 5.5mm;

[0019] 2) Fill the glass tube with an agarose molten solution containing the antibody to be tested. The volume of the molten agarose solution containing the antibody to be tested is 5 milliliters, and the volume of the antibody to be tested added to the molten agarose solution is 125 microliters;

[0020] 3) After the molten agarose solution is solidified, open the adhesive tape sealed at one end of the glass tube, and vertically stand the glass tube on a base with a water-absorbing material placed at the bottom;

[0021] 4) Add 0.01 ml of antigen of known concentration to the upper end of the vertical glass tube, and the concentration of the added antigen is 3 mg / ml;

[0022] 5) Aft...

Embodiment 2

[0029] A method for determining antibody titer using the antigen unidirectional diffusion method in this embodiment comprises the following steps:

[0030] 1) Seal one end of a graduated glass tube with adhesive tape, the inner diameter of the glass tube is 4mm, and the outer diameter is 5.5mm;

[0031] 2) pour the agarose molten solution containing the antibody to be tested into the glass tube of step 1), the volume of the molten agarose solution containing the antibody to be tested is 5 milliliters, and the volume of the antibody to be tested added in the molten agarose solution is 300 μL Microliter;

[0032] 3) After the molten agarose solution is solidified, open the adhesive tape sealed at one end of the glass tube, and vertically stand the glass tube on a base with a water-absorbing material placed at the bottom;

[0033] 4) Add 0.05 ml of antigen of known concentration to the upper end of the vertical glass tube, and the concentration of the added antigen is 3 mg / ml; ...

Embodiment 3

[0041] A method for determining antibody titer using the antigen unidirectional diffusion method in this embodiment comprises the following steps:

[0042] 1) Seal one end of a graduated glass tube with adhesive tape, the inner diameter of the glass tube is 4mm, and the outer diameter is 5.5mm;

[0043] 2) perfuse the agarose molten solution containing the antibody to be tested in the glass tube of step 1), the volume of the molten agarose solution containing the antibody to be tested is 4.8 milliliters, and the volume of the antibody to be tested added in the molten agarose solution is 240 Microliter;

[0044] 3) After the molten agarose solution is solidified, open the adhesive tape sealed at one end of the glass tube, and vertically stand the glass tube on a base with a water-absorbing material placed at the bottom;

[0045] 4) Add 0.03 ml of antigen of known concentration to the upper end of the vertical glass tube, and the concentration of the added antigen is 15 mg / ml; ...

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Abstract

The invention belongs to the technology field of a method for measuring antibody titer, and concretely relates to a method for determining antibody titer by using an antigen single immunodiffusion. The invention mainly solves the technology problems that according to the present determination method of antibody titer, the measurement results have deviations and the measurement process is complicated. According to the invention, the central single radial immunodiffusion used in the present determination method is restrained into a vertical radial single immunodiffusion in a calibrated glass tube, so that the Becker method deviation is eliminated, and the antibody titer is rapidly and accurately measured; a immunoprecipitation zone volume formed by single immunodiffusion can be directly read out from the scale on the calibrated glass tube, and thickness and diameter are not needed to be measured, so the result can be obtained more rapidly and accurately.

Description

technical field [0001] The invention belongs to the technical field of methods for measuring antibody titers, and in particular relates to a method for measuring antibody titers using an antigen one-way diffusion method. Background technique [0002] Antibody proteins are a type of defensive macromolecules widely expressed in higher animals, which can recognize and bind to pathogens (antigen molecules). Existing antibody titer (titer) determination methods are based on the characteristics of antibody and antigen affinity. Including ELISA method, agarose double diffusion method, Becker method and immunoturbidimetric method. In 1968, Becker published a method based on the principle of immunoprecipitation complexes formed by immunosingle diffusion of antigens and antibodies on agarose to determine antibody titers. In this method, the antigen with known mass is diffused in one direction on the agarose plate containing the antibody of unknown titer, so that the immune complex i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/559
CPCG01N33/559
Inventor 吴红艳苏少博李光飞
Owner 山西瑞亚力生物技术有限公司
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