Methods for the detection and quantification of nucleic acids within stretchable polymers
A technology of polymers and nucleic acids, applied in biochemical equipment and methods, measurement/testing of microorganisms, DNA preparation, etc., can solve problems such as unclear chewing gum to remove bacteria
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Embodiment 1
[0116] Example 1 describes the quantification of bacteria adhering to finger-rubbed chewing gum, Example 2 describes the quantification of bacteria adhering to chewing gum in vivo, and Example 3 describes other experiments for the quantification of bacteria adhering to chewing gum in vivo .
[0117] Example 1
[0118] Quantification of bacteria adhered to or entrapped in chewing gum in vivo
[0119] Further studies were conducted to confirm that chewing gum could remove bacteria from the oral cavity and that the bacteria's nucleic acid could be extracted.
[0120] Five healthy human volunteers (1 male, 4 female, aged 27 to 56 years) gave their written informed consent to participate in this study. The inclusion criteria for this study were that each volunteer was in good health and that their permanent teeth had at least 16 natural elements. The exclusion criterion was the use of antibiotics or mouthwash within one month prior to the study. In addition, the volunteers did ...
Embodiment 2
[0132] Additional experiments to quantify bacteria adhered to or entrapped in chewing gum in vivo
[0133] Further experiments were performed to determine the minimum time necessary to saturate the gum with bacteria and to quantify the bacteria adhered to the gum. The method was generally carried out as described in Example 1 above.
[0134] Five subjects were asked to chew spearmint gum for 2 weeks. Subjects were only allowed to chew one piece of gum per day. Store the chewed gum cuds in sterile 15 mL falcon tubes until PCR analysis. These samples are described in Table 3 below.
[0135] Table 3 - Chewing time and cud weight
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[0138] For analysis, frozen gum cuds were removed from the freezer and allowed to warm to room temperature. TE buffer (3ml) and chloroform (5ml) were added to the tube containing the gum cuds. The samples were mixed for 30 minutes and after the gum cud had completely dissolved, the samples were centrifuged at 4500 rpm for 1...
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