Anti-fatigue medicine and preparation method and application thereof
An anti-fatigue and drug technology, applied in the field of biotechnology, medicinal and edible plants, can solve problems such as slow onset of action, achieve the effects of small dosage, increase liver glycogen reserves, and prolong weight-bearing swimming time
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Embodiment 1
[0058] 1 Materials and methods
[0059] 1.1 Sample:
[0060] 1.2 Experimental animals: five-month-old middle-aged male ICR mice; eight-month-old old male C57 mice.
[0061] 1.3 Instruments and reagents: swimming box, lead wire, full-wavelength microplate reader, constant temperature water bath, centrifuge, liver glycogen detection kit (Nanjing Jiancheng, product number A043), lactic acid detection kit (Nanjing Jiancheng, product number A019-1) , Urea nitrogen kit (Nanjing Jiancheng, article number C013-1), SOD detection kit (Nanjing Jiancheng, article number A001-1), MDA detection kit (Nanjing Jiancheng, article number A003-1).
[0062] 1.4 Experimental method:
[0063] A. The first batch: 60 male five-month-old middle-aged ICR mice were randomly divided into 4 groups, 15 in each group. The low-dose group received 200 mg / kg intraperitoneal injection, the medium-dose 400 mg / kg intraperitoneal injection, and the high-dose 600 mg / kg intraperitoneal injection. The control grou...
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